Resources
Proteomics Databases
Metabolomics Databases
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• Identifying Protein-Protein Interactions
Identifying protein-protein interactions aims to recognize and confirm both physical and functional contacts between proteins. Proteins perform essential cellular functions and regulate biological processes by forming complex interaction networks. Protein-protein interactions (PPIs) are crucial in signal transduction, metabolic regulation, cell cycle control, immune responses, and more. Understanding PPIs aids not only in basic biological research but also in elucidating biological functions and disea......
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The antibody mapping is a dataset that uses high-throughput technologies to systematically analyze and document antibodies. It illustrates the distribution, characteristics, and functions of antibodies across various biological, tissue, cellular, and molecular contexts. Originating from the biological concept of an "atlas," it systematically presents the organizational and functional relationships of biological macromolecules. The construction and application of the antibody mapping significantly adva......
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• Antibody-Drug Conjugate Characterization
Antibody-drug conjugate characterization refers to the analytical techniques used to evaluate the structure, stability, purity, and functionality of ADCs. ADCs are targeted therapeutics composed of monoclonal antibodies, linkers, and small-molecule cytotoxic drugs. By leveraging the high specificity of antibodies, ADCs precisely deliver cytotoxic drugs to cancer cells, significantly improving the efficacy of anti-tumor treatments while minimizing off-target toxicity. Given the complex structure and di......
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Antibody CDR analysis is a vital technique in antibody engineering, vaccine development, and immunological research. The CDR refers to the variable regions of the antibody molecule, primarily responsible for the specificity of antigen binding. Each antibody consists of two heavy chains and two light chains, with three CDRs (CDR1, CDR2, and CDR3) in the variable regions of both chains. CDR3 is considered the critical region determining the antibody's affinity and specificity. Through antibody CDR analy......
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• Western Blot Protein Identification
Western blot protein identification is a widely utilized molecular biology technique essential for protein analysis. This method allows for the separation, transfer, and detection of proteins within complex biological samples by employing antibodies that specifically recognize target proteins, in combination with electrophoresis and membrane transfer methods. Western blot protein identification not only detects the presence of particular proteins but also provides valuable insights into their relative......
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• Western Blot Protein Analysis
Western Blot protein analysis is a classical technique for protein detection, integrating steps of protein electrophoresis separation, membrane transfer, specific antibody recognition, and signal detection. It is employed to assess the expression of specific proteins, encompassing their presence, relative abundance, molecular weight, and post-translational modifications like phosphorylation and acetylation. Due to its high specificity and sensitivity, Western Blot is a pivotal tool in studying protein......
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• Unknown Protein Identification in Proteomics Research
Unknown protein identification is a pivotal task in proteomics research, focusing on the detection of unidentified proteins in biological samples through advanced analytical technologies, and elucidating their roles in biological systems. This process predominantly leverages mass spectrometry in conjunction with bioinformatics to achieve efficient and precise protein identification. The scope of unknown protein identification is broad, spanning fundamental biological research, disease mechanism invest......
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Top-down protein analysis is a method that directly examines intact proteins using high-resolution mass spectrometry to determine molecular weight, post-translational modifications, and protein isoforms. This approach offers several advantages over traditional bottom-up methods. By avoiding enzymatic digestion, it prevents the loss of protein fragments, preserving the protein's original information. Furthermore, it can concurrently identify various post-translational modifications such as phosphorylat......
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TMT protein quantification is a mass spectrometry-based technique designed for high-throughput protein quantification using tandem mass tags for the relative quantification of multiple samples. Compared to traditional methods such as Western blotting and ELISA, TMT protein quantification offers superior sensitivity and precision, making it ideal for extensive protein expression analysis in complex biological samples. The technique centers on the chemical labeling of proteins with TMT tags and subseque......
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The TMT labeling mass spec is a cutting-edge protein quantitation method utilizing isotope labeling coupled with high-resolution mass spectrometry. The technique leverages isotopic characteristics of TMT tags to achieve multiplex quantitation. A TMT tag is composed of three critical components: a reactive group that covalently binds to peptides, a mass normalization region ensuring uniform physicochemical properties across different tags, and a reporter ion used for quantitation. During the mass spe......
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