Resources
Proteomics Databases
Metabolomics Databases
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• How to Perform High-Throughput Peptidomic Analysis with LC-MS/MS?
Peptidomics is a scientific discipline focused on the study of endogenous peptides (typically <10 kDa) present in biological specimens. These peptides play critical roles in diverse physiological processes, including neurotransmission, immune response, hormonal signaling, and metabolic regulation, and hold substantial potential as functional molecules and biomarkers. Compared with proteomics, peptidomics provides a closer reflection of the actual states of protein cleavage, processing, and .......
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• How to Identify Active Enzyme Targets Using ABPP Technology?
Activity-Based Protein Profiling (ABPP) is a proteomic technique that enables the analysis of proteins based on their active sites. By covalently labeling functionally active proteins with specific small-molecule probes, ABPP allows for the efficient and accurate identification of catalytically active enzymes, providing a transformative tool for target discovery and elucidation of drug mechanisms. Fundamental Principles of ABPP Technology for Active Enzyme Targets Identification 1. Definition of Active.....
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• How to Choose the Right Peptide Sequencing Method? Edman Degradation vs. Mass Spectrometry
Peptide sequencing is an essential approach for elucidating the primary structure of proteins, and it has been widely applied in protein identification, validation of novel sequences, localization of modification sites, and antibody development. Currently, Edman degradation and mass spectrometry (MS) represent the two mainstream peptide sequencing methods, each offering distinct advantages tailored to different experimental requirements. This article provides a systematic comparison of these methods in.....
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• How to Simultaneously Determine Protein Purity and Concentration?
In life sciences research and biopharmaceutical manufacturing, protein purity and concentration are essential parameters for assessing sample quality and process stability. Protein concentration reflects the absolute amount of the target protein in a sample, whereas purity denotes the proportion of the target protein relative to impurities. Conventionally, these two indicators are determined using distinct analytical methods, which not only increase procedural complexity but also heighten the risk of ......
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• How to Analyze Protein Purity and Homogeneity Using SEC-HPLC?
In biopharmaceutical and life science research, protein purity and homogeneity are critical quality attributes (CQAs) that directly influence the safety, efficacy, and stability of biological products. Size-exclusion high-performance liquid chromatography (SEC-HPLC), owing to its high resolution, gentle separation conditions, and robust quantitative capability, has become an indispensable technique for assessing protein purity and homogeneity. This article outlines the principles of SEC-HPLC, key......
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• How Protein Primary Structure Data Facilitates Precision Drug Design?
In the domains of precision medicine and novel drug development, protein primary structure data (amino acid sequence information) represents not only a cornerstone of fundamental biological research but also a critical driver of precision drug design. Systematic analysis of protein sequences enables researchers to gain a fundamental understanding of the structural and functional properties of target proteins, thereby accelerating the transition from target identification to drug optimization. Amino Acid....
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• How to Choose the Right Method for Protein Structure Determination?
Proteins play essential roles in virtually all biological processes. Their functions, ranging from enzymatic catalysis and signal transduction to structural support within cells, are fundamentally dependent on their three-dimensional structures. However, amino acid sequences alone are insufficient to fully elucidate their biological functions. At the molecular level, protein structures not only reveal functional mechanisms but also provide atomic-level insights that enable applications such as drug target..
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In structural biology and protein research, Circular Dichroism (CD) spectroscopy represents a key technique widely employed for secondary structure determination, monitoring conformational transitions, and assessing thermal stability. CD spectroscopy provides a rapid and non-destructive approach to probing protein folding states. However, accurate interpretation of CD data critically depends on computational tools and algorithms. Among the most widely used platforms for CD data analysis are BeStSel and.....
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• A Comprehensive Analysis of CD Spectroscopy Methods for Studying the Protein Folding Process
Protein folding is among the most fundamental yet complex processes in biology. Correct folding ensures that proteins acquire specific three-dimensional structures and biological functions, whereas misfolding can result in a range of pathological conditions, including Alzheimer’s disease and prion-related disorders. In structural biology and drug discovery, real-time monitoring of protein folding states is a critical approach for elucidating functional mechanisms and optimizing molecular stability. Ci......
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Glycoproteins are widely distributed across various biological systems and play essential roles in processes such as cell recognition, signal transduction, and immune regulation. Their structural complexity primarily arises from the diversity of glycan moieties and the distribution of modification sites, which poses significant challenges for glycoprotein structure characterization. Among the available structural analysis techniques, Circular Dichroism (CD) spectroscopy has been extensively employed for....
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