Resources
Proteomics Databases

Metabolomics Databases

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• Protein Glycosylation Analysis
Protein glycosylation analysis techniques are essential for investigating the glycosylation modifications on proteins. By examining the glycosylation states and glycan structures on protein molecules, researchers can gain insights into how this vital post-translational modification influences biological functions. Glycosylation is one of the most prevalent and intricate post-translational modifications and plays a role in numerous biological processes, such as cell-cell communication, protein folding,......
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Protein gel analysis is a classical and widely utilized technique in protein research, instrumental in separating and analyzing protein components in biological samples. This method allows researchers to visually assess the molecular weight distribution, purity, and expression levels of proteins, providing a foundation for further functional studies and quantitative analyses. The core principle of protein gel analysis involves the use of electrophoresis to separate proteins based on molecular weight o......
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• Protein Identification by LC-MS
Protein identification by LC-MS stands as a cornerstone technology in modern proteomics, combining the effective separation capability of liquid chromatography (LC) with the sensitive detection of mass spectrometry (MS). This technique enables high-throughput protein identification and characterization in complex samples by breaking down proteins into peptides, which are then individually separated and detected for precise molecular-level analysis. Initially, samples undergo protein extraction and enz......
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• Protein Expression Profiling
Protein expression profiling is a scientific approach used to analyze the composition and abundance changes of proteins within cells, tissues, or whole organisms. This research area is crucial for understanding biological functions, as proteins are the direct functional molecules responsible for carrying out life processes. By implementing protein expression profiling, scientists can identify protein groups that exhibit differential expression under varying physiological or pathological conditions, wh......
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• Protein Expression Proteomics
Protein expression proteomics is a field dedicated to exploring the expression levels and dynamic changes of proteins within organisms. At its core, it employs advanced experimental techniques and bioinformatics to elucidate the expression patterns and functional significance of proteins under various biological conditions. In living systems, proteins, which are products of gene expression, serve as crucial molecules that execute diverse biological functions. The expression levels of these proteins ar......
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• Protein Crosslinking Mass Spectrometry
Protein crosslinking mass spectrometry (PCMS) is a powerful technique for investigating protein-protein interactions and their three-dimensional structures. This method employs chemical crosslinkers to form covalent bonds between amino acid residues of adjacent proteins or within specific sites of a protein. Such crosslinking stabilizes protein complexes, allowing researchers to analyze their structure and function. Mass spectrometry then examines these crosslinked molecules, revealing precise interac......
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• Protein Correlation Profiling
Protein correlation profiling is a method used to investigate the interactions and functional relationships between proteins, aimed at understanding how proteins collaborate to carry out complex biological functions. In cellular environments, proteins engage in complex interaction networks, forming functional modules rather than acting in isolation. These modules are essential in processes such as signal transduction, gene regulation, and metabolic control. Protein correlation profiling can dissect th......
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• Protein Coverage Mass Spectrometry
Protein coverage mass spectrometry is a high-resolution analytical method utilized to assess the coverage of protein amino acid sequences, extensively used in proteomics research and biopharmaceutical development. This technique enables the precise analysis of protein peptide fragments via mass spectrometry, producing coverage maps to verify whether a protein's complete or partial sequence is detected. The extent of protein coverage is indicative of the analysis's depth and precision, crucial for eluc......
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• Protein Concentration Analysis
Protein concentration analysis is a technique aimed at determining the protein content in biological samples, with widespread applications in life sciences research and biopharmaceutical development. Proteins, as fundamental components of biomolecules, are central to cellular functions, signal transduction, and metabolic regulation. Accurate protein concentration measurement is essential not only for basic biological experiments but also for applications such as disease diagnosis, drug development, an......
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• Advances in Protein Content Analysis Methods
Protein content analysis is a critical technique for quantitatively measuring the total amount of protein in a sample, and it is fundamental to life sciences research and industrial applications. As key mediators of biological functions, proteins maintain cellular processes, regulate physiological activities, and enable adaptation to environmental changes. Analyzing protein content allows researchers to obtain essential biological information about a sample, supporting subsequent functional studies, d......
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