Resources
Proteomics Databases
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Metabolomics Databases
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• Protein-Protein Interaction Detection Methods and Analysis
Protein-Protein Interaction Detection Methods and Analysis are crucial components of life sciences research, designed to uncover the mechanisms of interaction between proteins and other molecules, such as nucleic acids, small molecules, and ions, and to understand their functional roles. Protein-protein interactions are essential for intracellular processes such as signal transduction, metabolic regulation, and the maintenance of physiological functions. Investigating these interactions provides valua......
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Protein profiling in plants is a high-throughput mass spectrometry-based technique used to study the composition, expression levels, post-translational modifications, and functional properties of plant proteins. As a critical component of proteomics research, protein profiling in plants provides essential data to uncover mechanisms underlying plant growth, development, metabolism, and environmental responses. Unlike traditional methods, this approach comprehensively addresses the complexity of plant p......
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• Protein Profiling by SDS-PAGE
Protein profiling by SDS-PAGE is a widely utilized experimental technique grounded in polyacrylamide gel electrophoresis, essential for protein research. SDS (sodium dodecyl sulfate) is an anionic surfactant that denatures proteins by disrupting their native structures and imparts uniform negative charges, ensuring that protein separation during electrophoresis is determined solely by molecular weight. PAGE (polyacrylamide gel electrophoresis) provides a polymeric gel matrix optimized for high-resolut......
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• Protein and Nucleotide Sequence Analysis
Protein and nucleotide sequence analysis is a foundational technology in life sciences, enabling researchers to unravel how genetic information encodes proteins and dictates their functions. Nucleotide sequences govern the arrangement of amino acids in proteins, which in turn determine their three-dimensional structures and functional properties. Protein and nucleotide sequence analysis not only provides a basis for decoding the genetic code but also serves as an indispensable tool in understanding di......
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N-terminal analysis, the determination of the N-terminal amino acid sequence, is employed to identify and characterize the sequence of proteins or polypeptides. The N-terminus, the end of a protein or polypeptide with a free amino group, plays a pivotal role in protein biosynthesis and functionality. This technique has wide-ranging applications in protein identification, post-translational modification studies, and biomarker discovery. In the development of biopharmaceuticals, N-terminal analysis help......
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• N-Glycan Analysis of Monoclonal Antibodies
N-glycan analysis of monoclonal antibodies is a critical technique in proteomics research. Monoclonal antibodies, produced by a single B-cell clone, exhibit exceptional specificity and uniformity, making them indispensable in the treatment of cancer, autoimmune diseases, and infectious diseases. N-glycans are sugar chains covalently attached to the nitrogen atom of amino acid residues within proteins, significantly influencing protein structure and function. By analyzing the types and structures of N-......
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• N-Terminal Amino Acid Sequencing
N-terminal amino acid sequencing is a technique used to determine the N-terminal amino acid sequence of proteins or polypeptide chains. Proteins play a critical role in various biological processes, with their functions, structures, and stabilities often closely tied to their amino acid sequences. This method provides researchers with essential preliminary sequence data, offering insights into the biological functions of proteins. Primarily applied in proteomics research, N-terminal amino acid sequenc......
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• LC-MS/MS Protein Sequencing in Proteomics
LC-MS/MS protein sequencing is a powerful analytical technique for identifying and quantifying proteins in biological samples. By integrating the high-resolution separation capabilities of liquid chromatography (LC) with the sensitivity and specificity of tandem mass spectrometry (MS/MS), this method enables efficient separation, precise identification, and accurate quantification of proteins in complex biological mixtures. LC separates complex protein samples into smaller components, facilitating sub......
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• N and C Terminal Amino Acid Determination
N and C terminal amino acid determination refers to the analytical process of identifying and characterizing the specific amino acid sequences at the N-terminal (amino terminus) and C-terminal (carboxyl terminus) of proteins using biochemical and mass spectrometry techniques. The N-terminal is the first amino acid in a polypeptide chain, while the C-terminal represents the last. By determining the sequences at these terminals, researchers can uncover critical information about protein function, evolut......
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• LC-MS/MS Analysis of Proteins
LC-MS/MS analysis of proteins is a widely applied analytical technique in proteomics, integrating the high-resolution separation capability of liquid chromatography (LC) with the high sensitivity and specificity of tandem mass spectrometry (MS/MS). This approach enables comprehensive and detailed analysis of proteins in complex biological samples. The key advantage of LC-MS/MS analysis of proteins lies in its ability to simultaneously identify and quantify thousands of proteins in complex mixtures, pr......
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