Resources

Proteomics Databases



Metabolomics Databases

• • Proteomics Analysis of Exosome Samples Based on LC-MS/MS

  Exosomes are nanoscale vesicles secreted by cells, containing proteins, lipids, nucleic acids, and other biological molecules. They play a crucial role in intercellular communication, immune regulation, tumor metastasis, and various physiological and pathological processes, making them a hotspot in biomedical research.

• • Quantitative Analysis of Proteomics in Subcellular Fractions

  Quantitative analysis of proteomics in subcellular fractions is a critical technique for elucidating the distribution and functional activities of proteins in different regions of a cell. Cells are complex structures, with each subcellular component, such as the nucleus, mitochondria, endoplasmic reticulum, and plasma membrane, exhibiting distinct forms and functions.

• • Structural Characterization of N-Glycan Chains Using Permethylation and Mass Spectrometry

  N-glycan chains, as part of protein post-translational modifications (PTMs), play crucial roles in cell recognition, signal transduction, and immune responses. Understanding the structure of N-glycans is vital for uncovering their biological functions. However, due to the complexity of N-glycans, structural characterization has been a significant challenge in glycomics research.

• • Mechanism of Peptide Structure Determination

  Peptide structure determination is fundamental to understanding protein functions and biological processes. An accurate determination of peptide structures is essential for studying protein functions, molecular interactions, and their role in diseases. The process involves determining the peptide’s primary structure, resolving spatial conformation, identifying molecular interactions, and characterizing post-translational modifications (PTMs).

• • Workflow of Peptide Structure Determination

  Determining peptide structure is essential for elucidating the amino acid sequence and spatial conformation, which is critical for biological research and drug development. This process includes several steps, from sample preparation to final data analysis, with each step impacting the experiment's accuracy and success.

• • Application of Peptide Structure Determination

  Peptides are molecules formed by the linkage of amino acids through peptide bonds, playing numerous vital biological roles within organisms. Peptides not only constitute hormones, neurotransmitters, enzyme inhibitors, and more, but they also function as signaling molecules, antibiotics, and immune modulators. Therefore, determining peptide structure is essential for understanding their biological functions, developing new drugs, and uncovering biochemical mechanisms.

• • Principle of Peptide Structure Determination

  Peptides are linear molecules composed of amino acids linked by peptide bonds, playing vital roles in numerous biological functions. Determining the structure of peptides is crucial for understanding their functions, as the primary structure (amino acid sequence), secondary structure (local folding), tertiary structure (overall three-dimensional conformation), and quaternary structure (complexes of multiple peptide chains) significantly influence their biological activity.

• • Mechanism of Peptide Identification

  Peptide identification is one of the key steps in modern proteomics research, widely applied in biomedical, drug development, and basic biological studies. At its core, peptide identification relies on mass spectrometry (MS) techniques to accurately identify and quantify peptides, which allows for the deduction of corresponding protein information.

• • Application of Peptide Identification

  Peptide identification is a core technology in proteomics, widely used in protein sequence analysis, structural and functional studies of proteins, and the understanding of various biological processes. With advancements in mass spectrometry, the accuracy and sensitivity of peptide identification have significantly improved, providing an effective means to analyze proteins in complex biological samples.

• • Workflow of Peptide Identification

  Peptide identification is a vital procedure in proteomics research, widely used for protein identification and quantification in biological studies. The workflow encompasses key steps such as sample preparation, enzymatic digestion, peptide separation, mass spectrometry (MS) analysis, and data processing. Each of these steps is essential for ensuring accurate and reproducible results. Below is a refined and more coherent explanation of the peptide identification workflow.