Resources

Proteomics Databases



Metabolomics Databases

• • Protein Complex Analysis

  Protein complex analysis is a technique used to study the interactions between proteins and their compositions. By revealing the structure, function, and dynamic changes of protein complexes, it helps scientists understand the complex biological processes occurring within organisms. A protein complex refers to a functional unit formed by two or more proteins through specific physical and chemical interactions. These complexes perform a variety of biological functions within the cell, such as signal tr......

• • Protein Composition Analysis

  Protein composition analysis is a crucial methodology for investigating the variety, abundance, and dynamic alterations of proteins in biological samples. By elucidating the complex network and functional mechanisms of proteins, it drives forward both fundamental research and applied developments in life sciences. As essential components of living organisms, proteins participate in nearly all biological processes. This analysis assists scientists in understanding cellular molecular changes under diffe......

• • Plant Protein Complete Amino Acid Profile

  Plant protein complete amino acid profile is a key research area for assessing the nutritional value and functional characteristics of plant proteins. With advantages such as abundant sources and environmental sustainability, plant proteins have become a popular choice for human dietary protein and industrial applications. Amino acids are the basic building blocks of proteins, and their types and proportions directly determine the quality, functionality, and potential applications of plant proteins. C......

• • Peptide Fragment Fingerprinting

  Peptide fragment fingerprinting (PFF) is a crucial tool for analyzing protein structures and identifying proteomes. Utilizing mass spectrometry, this technique analyzes peptides derived from protein hydrolysis to produce distinctive mass spectra. Each protein generates a unique peptide fragment fingerprint during mass spectrometry analysis, akin to a personal fingerprint. This distinctiveness makes PFF an essential method for protein identification and quantification in proteomic research. PFF plays a......

• • Western Blot Band Quantification

  Western blot band quantification is one of the key techniques in protein research, primarily used to detect the expression levels of specific proteins and to perform relative or absolute quantification based on the grayscale intensity of bands. Western blot (WB) technology is based on protein electrophoretic separation, antibody-specific recognition, and chemical or fluorescent signal detection, enabling the precise identification of target proteins within complex samples. Compared to traditional West......

• • N-Terminal Sequencing in Protein Analysis

  N-terminal sequencing, the process of determining an amino acid sequence starting from the N-terminal (amino-terminal) of a protein, uses chemical or enzymatic degradation methods to identify the sequence of amino acids in a protein or polypeptide chain. This technique is widely used in biological research. In the discovery and identification of novel proteins, N-terminal sequencing provides direct information on amino acid sequences, which assists in predicting protein function and structure. Underst......

• • Parameters Required to Characterize a Protein

  Proteins are fundamental to biological processes in living organisms and serve as the primary components of biological organs and tissues. Protein characterization, which involves obtaining specific structural and functional information, is a critical step in biological research. A comprehensive characterization of a protein typically requires the determination of the following parameters: sequence information, molecular weight, secondary structure, tertiary structure, quaternary structure, hydrophobicity..

• • Deglycosylation in Protein Mass Spectrometry

  Protein mass spectrometry is an essential technique in biotechnology for analyzing protein composition, structure, and properties. In protein mass spectrometry, deglycosylation is commonly used to remove glycan moieties from proteins, allowing determination of their intact molecular weight. Mass spectrometry analysis following deglycosylation provides key insights, including the intact molecular weight of the protein, the glycosylation sites, and structural information.

• • What Purity Is Required for the Identification of Polypeptide Secondary Structure

  Peptide secondary structure identification relies on sample purity as a key parameter, as the degree of purity directly influences the accuracy and reliability of experimental outcomes. Purity Requirements: To achieve accurate and reproducible results, peptide samples should have a minimum purity of 90%. The presence of significant impurities in a sample can interfere with experimental outcomes, leading to deviations in identification accuracy. In certain cases, particularly for structurally complex.......

• • Preparation of Single Cell Suspensions for Mass Cytometry

  Materials and Instruments: 1. Experimental cell lines 2. Phosphate-buffered saline (PBS) 3. 0.25% Trypsin-EDTA 4. Centrifuge 5. Flow cytometer Preparation Procedure: 1. Cell Washing and Harvesting: Begin by washing the cells with PBS. Following this, treat the cells with 0.25% Trypsin-EDTA to facilitate cell detachment and harvesting.