Resources

Proteomics Databases



Metabolomics Databases

• • Protein Gel Identification: Selection and Comparison of Co-IP and CO-IP-MS Techniques

  Protein-protein interactions are crucial for understanding biological processes and disease mechanisms. Co-immunoprecipitation (Co-IP) followed by mass spectrometry (MS) is a commonly used method to study protein interactions.

• • Mechanism of Ion Mobility in 4D Proteomics

  Advancements in proteomics have opened new avenues for understanding the intricate workings of biological systems. However, conventional proteomics methods encounter significant obstacles when it comes to detecting low-abundance proteins due to inherent technical limitations. Detecting and quantifying these proteins is crucial because they play pivotal roles in various biological processes.

• • Application of 4D Proteomics in Low-Abundance Protein Detection

  Proteomics research seeks to comprehensively understand the structure, function, and interactions of proteins within biological systems. However, traditional proteomics techniques face significant challenges in detecting low-abundance proteins, which often play crucial roles in biological processes. The emergence of 4D proteomics has provided a groundbreaking solution to these challenges.

• • Workflow of 4D Proteomics for Proteome-Wide Protein Quantification

  As proteomics research advances, global proteome quantification techniques have become essential tools in life sciences. Traditional proteomics techniques primarily rely on two-dimensional liquid chromatography (2D-LC) coupled with mass spectrometry (MS). However, in recent years, 4D proteomics has gained significant attention for its enhanced resolution and sensitivity.

• • Principle of 4D Proteomics

  With the rapid advancement of biotechnology, proteomics has become a crucial tool for understanding the functions of biological systems. Proteomics aims to comprehensively analyze the complete set of proteins within cells, tissues, or organisms. However, the inherent complexity and dynamic nature of proteins pose significant challenges for traditional proteomics methods.

• • Advantages and Disadvantages of 4D Proteomics in Low-Abundance Protein Detection

  4D proteomics is an advanced method that combines multidimensional separation techniques with high-resolution mass spectrometry analysis. Compared to traditional proteomics, 4D proteomics offers enhanced capabilities in capturing and analyzing proteins, especially in the detection of low-abundance proteins. Despite its many strengths, the application of 4D proteomics to low-abundance protein detection presents certain challenges.

• • Mechanism of Top-Down Proteomics in PTM Characterization

  Proteomics is a scientific discipline dedicated to the study of proteins' structure, function, and interactions within a biological system. In recent years, Top-Down Degradation has emerged as a crucial method for studying protein structure and function, garnering widespread attention. Unlike traditional bottom-up approaches, Top-Down Degradation directly analyzes intact proteins, providing more detailed structural information.

• • Application of Top-Down Proteomics in PTM Detection

  Proteomics, the comprehensive study of proteins within a biological system, is a critical branch of the post-genomic era, offering essential tools to uncover the essence of life processes. N-terminal degradation, a method of protein modification and degradation, holds significant promise within proteomics.

• • Workflow of Top-Down Proteomics for Protein Analysis

  Top-Down Proteomics is a strategy in proteomics that involves the direct analysis of intact proteins. Unlike traditional Bottom-Up Proteomics, which requires the digestion of proteins into peptides, Top-Down Proteomics preserves the protein's original form, making it particularly suitable for studying post-translational modifications, protein isoforms, and protein complexes.

• • Advantages and Disadvantages of Top-Down Proteomics

  Top-down proteomics, as an advanced methodology, has carved out a niche for itself in the field of proteomics by offering insights that other techniques may miss. However, the complexity and limitations associated with this approach warrant a deeper understanding of its practical applications and potential areas for improvement.