Resources

Proteomics Databases



Metabolomics Databases

• • Requirements for Samples in Protein Immunoprecipitation-Mass Spectrometry

  Immunoprecipitation-Mass Spectrometry (IP-MS) is a robust technique for analyzing protein-protein interactions. Achieving reliable and reproducible results hinges on the quality and handling of the samples. The following outlines the sample requirements for IP-MS concerning "purity, integrity, quantity, buffer, and antibody selection." 1. Sample Purity: Samples need to be adequately pure to minimize nonspecific protein interactions. Prior to IP-MS, samples should be subjected to thorough washing and........

• • Conditions for Protein Mass Spectrometry Quantification

  Protein mass spectrometry quantification is a crucial analytical approach for investigating protein structures and functions. This method synthesizes theoretical knowledge, experimental techniques, instrumentation, data processing, and experimental design with result interpretation, thereby providing robust tools and methodologies for comprehensive protein research.

• • Can Mass Spectrometry Measure Large Proteins

  Mass spectrometry (MS) is a powerful analytical technique that enables precise mass measurement and structural characterization of biomolecules. In biomedical research, MS has become an indispensable tool for the identification and quantification of proteins and other macromolecules. Principles and Methods: MS operates based on the mass-to-charge ratio (m/z) of ionized molecules. The process involves ionizing the sample, accelerating the resulting ions in an electric or magnetic field, and measuring their..

• • Calculation of m/z for Polypeptides in Mass Spectrometry

  Calculation of m/z for Peptides in Mass Spectrometry: In mass spectrometric analysis, the mass-to-charge ratio (m/z) is a fundamental parameter for identifying ions in a spectrum. Each peak represents an ion, and its m/z value is derived by dividing the mass of the ion by its charge. Typically, the charge (z) is +1 or -1, as most ions are singly charged. However, larger molecules, such as proteins and peptides, may exhibit multiple charges, significantly affecting their ionization.

• • Protein Analysis Using Liquid/Gas Chromatography-Mass Spectrometry

  Mass spectrometry is a powerful analytical technique used to determine the mass, chemical structure, and purity of a sample. In biological research, it is widely employed for the identification of proteins and other macromolecules. By providing detailed structural and compositional information, mass spectrometry plays a crucial role in advancing our understanding of these complex biomolecules.

• • Naming of Gene Knockout Cell Lines

  In molecular biology, gene knockout is an experimental technique used to disable the function of a specific gene in an organism through genetic engineering. This method is valuable for investigating the roles of particular genes and their potential links to diseases. In cell culture, gene knockout cell lines are created by genetically engineering cells to inactivate specific genes. These cell lines serve as powerful tools for studying disease mechanisms, drug screening, and understanding gene functions.

• • Immunoprecipitation Mass Spectrometry Protocol

  Immunoprecipitation Mass Spectrometry (IP-MS) is a widely used technique for identifying protein-protein interactions, integrating immunoprecipitation with mass spectrometry-based proteomic analysis. 1. Sample Preparation: Protein extraction is performed from cells or tissues by lysing the samples with an appropriate lysis buffer, such as radioimmunoprecipitation assay (RIPA) buffer, followed by centrifugation to remove cell debris and isolate soluble proteins.

• • Olink Protein Detection

  Olink protein detection is an innovative, highly sensitive, and high-throughput technology developed by the Swedish company Olink, designed for the precise quantification of thousands of proteins in biological samples. This method is based on the Proximity Extension Assay (PEA), which utilizes DNA-conjugated antibodies to achieve exceptional specificity and sensitivity in protein detection.

• • Protein Extraction for TMT/iTRAQ

  In biomedical research, proteomics is a critical field that focuses on the large-scale study of proteins. Among the widely used protein quantification techniques, Tandem Mass Tag (TMT) and Isobaric Tags for Relative and Absolute Quantitation (iTRAQ) allow for precise protein quantification and large-scale protein sample analysis. Principles of TMT and iTRAQ: Both TMT and iTRAQ are isotope-labeling techniques for protein quantification. These methods involve the attachment of isotope tags to peptides........

• • ADA Antibody PK Analysis

  ADC (Antibody-Drug Conjugate) is an innovative targeted therapeutic agent that selectively eliminates tumor cells through the specific binding of antibodies and the potent cytotoxic effect of the conjugated drug. The pharmacokinetic (PK) analysis of ADC is crucial for elucidating its distribution, metabolism, and excretion within the body, thereby informing and optimizing its clinical application.