Resources

Proteomics Databases



Metabolomics Databases

• • GST Fusion Protein Pull-Down Assay

  GST fusion protein pull-down assay is a widely used biochemical technique for studying protein-protein interactions. GST (glutathione S-transferase) is an enzyme that specifically binds to glutathione (GSH). By fusing the target protein with GST, the interaction between GST and GSH can be utilized to purify and separate the protein. The basic principle of the GST fusion protein pull-down assay involves fusing GST with the target protein, expressing it, and immobilizing the fusion protein on GSH resin ......

• • Cross-Linking Protein Interaction Analysis

  Cross-linking protein interaction analysis is a technique used to investigate the physical interactions between proteins and their roles in biological systems. Protein interactions are integral to virtually all biological processes, such as signal transduction, metabolic pathways, and gene expression regulation. By decoding these interactions, scientists can unveil complex cellular networks, which are pivotal for understanding disease mechanisms, developing new drugs, and identifying biomarkers. For i......

• • Edman Degradation N-Terminal Sequencing

  Edman degradation N-terminal sequencing is a well-established method for determining the N-terminal amino acid sequence of protein chains. Developed by Pehr Edman in the 1950s, this technique quickly became a cornerstone in protein chemistry. Through a series of chemical reactions, Edman degradation sequentially removes and identifies N-terminal amino acids, allowing researchers to elucidate the primary structure of proteins over time. In proteomics, understanding a protein’s amino acid sequence is cr......

• • Co-Immunoprecipitation Pull-Down Assay

  Co-immunoprecipitation pull-down assay is an established technique for analyzing protein-protein interactions. This method captures protein complexes interacting with a target protein through specific antibodies binding to it. Widely employed in molecular biology, the assay enables the verification of protein complex existence and composition. For instance, examining interactions between transcription factors and DNA-binding proteins enhances our understanding of gene expression regulation mechanisms.......

• • Collagen Analysis by HPLC

  Collagen analysis by HPLC is a widely utilized technique in proteomics research. The principle of collagen analysis by HPLC lies in the high resolution and sensitivity offered by liquid chromatography. This method involves preparing the sample through dissolution, filtration, and injection into the HPLC system. The sample is then separated within the chromatographic column, where it interacts with the stationary and mobile phases. Each component is progressively eluted based on its partitioning behavi......

• • Co-Immunoprecipitation and Pull-Down Assays

  Co-immunoprecipitation and pull-down assays are pivotal techniques for investigating protein-protein interactions. These methods are fundamental in modern molecular biology and proteomics research, facilitating the exploration of intricate cellular protein networks. Co-IP is predominantly used to verify established protein interactions or to detect unknown interacting partners within the same biological pathway. Pull down assays employ a tagged "bait" protein to isolate "prey" proteins that bind to it......

• • Co-Immunoprecipitation Protein-Protein Interaction

  Co-immunoprecipitation protein-protein interaction is a widely used experimental technique in biochemical and molecular biology research. It involves capturing the target protein and its interacting partners using specific antibodies, enabling the identification and analysis of protein complexes. The typical Co-IP process includes three main steps: first, selecting an antibody that specifically binds to the target protein; second, using the antibody to capture the target protein, followed by precipita......

• • Amino Acid Analysis of Proteins

  Amino acid analysis of proteins is a technique for the qualitative and quantitative detection of amino acid composition and modifications within proteins. Amino acids are the basic building blocks of proteins, and their specific sequence determines the protein's structure and function. By performing amino acid analysis of proteins, researchers can uncover insights into protein structure, function, and changes associated with diseases or metabolic disorders. This technology is widely used in proteomics......

• • Amino Acid Sequence Analysis

  Amino acid sequence analysis is a technique used to determine the order of amino acids in protein molecules. The structure and function of proteins are directly determined by their amino acid sequence, with amino acids linked by peptide bonds in a specific order to form protein molecules with distinct three-dimensional structures. The key advantages of amino acid sequence analysis include high sensitivity, high resolution, and high throughput. Mass spectrometry, in particular, is well-suited for analy......

• • Acetylation Mass Spectrometry

  Acetylation mass spectrometry is a specialized mass spectrometry technique for analyzing the post-translational modifications (PTM) of proteins, focusing specifically on the detection and identification of acetylation sites and performing both qualitative and quantitative analyses. Acetylation is a crucial PTM, prevalent in both eukaryotic and prokaryotic proteins, where it regulates key biological processes including gene expression, metabolic pathways, the cell cycle, and signal transduction. The de......