Resources

Proteomics Databases



Metabolomics Databases

• • PhIP-Seq Analysis: Normalization, Background Control, and Hit Calling Decisions

  Technical guide for PhIP-Seq analysis normalization, background control, and hit calling decisions.

• • How to Interpret PhIP-Seq Results for Autoantibody Discovery and Candidate Validation

  Decision-focused guide to phip seq data analysis for autoantibody discovery, candidate triage, and orthogonal validation planning.

• • PhIP-Seq Data Analysis Workflow: From Read Counts to Enriched Peptide Hits

  Technical guide for PhIP-Seq Data Analysis Workflow: From Read Counts to Enriched Peptide Hits.

• • What Is PhIP-Seq? A Practical Guide to When Research Teams Choose It

  Learn what PhIP-Seq measures, how Phage ImmunoPrecipitation Sequencing works, and when research teams choose it over ELISA or other targeted assays for exploratory antibody profiling.

• • Crosstalk between Lactylation and Other PTMs

  Lactate was once regarded as a metabolic by-product of glycolysis, but recent studies have fundamentally changed this understanding. In 2019, Zhang et al. first reported histone lactylation in Nature, demonstrating that lactate can participate in epigenetic regulation by covalently modifying lysine residues on histones. This discovery opened a new direction in the study of post-translational modifications (PTMs) and prompted extensive investigation into the synergistic and competitive mechanisms betwe......

• • What Is the Role of SIRT5 in Histone Malonylation?

  With the continued advancement of epigenetic regulation research, the study of histone post-translational modifications (PTMs) has expanded beyond classical acetylation and methylation to include a range of emerging lysine acylation modifications. Malonylation is one such modification that has attracted increasing attention in recent years and is closely associated with cellular metabolic status. As a predominantly mitochondria-localized deacylase, SIRT5 plays a critical role in regulating protein mal......

• • PhIP-Seq for Cohort Studies: Designing Case-Control Comparisons and Antibody Signature Discovery

  Learn when PhIP-Seq is the right choice for cohort-based antibody discovery, how to structure case-control comparisons, and how to define candidate antibody signatures with defensible analysis and validation planning.

• • How to Choose a Peptide Library for PhIP-Seq: Proteome-Wide, Pathogen-Focused, and Custom Designs

  Choose the right PhIP-Seq peptide library by matching study goals to proteome-wide, pathogen-focused, or custom designs.

• • Common PhIP-Seq Troubleshooting Questions: Weak Enrichment, Background Signals, and Library Coverage

  Learn how to troubleshoot weak enrichment, background signal, and uneven library coverage in PhIP-Seq data by separating assay setup issues from library performance, sequencing allocation, and analysis threshold problems.

• • Interpreting PhIP-Seq Results: Enriched Peptides, Cross-Reactivity, Controls, and Biological Relevance

  Technical guide for interpreting enriched peptides, controls, cross-reactivity, and validation readiness in phip seq analysis.