Resources

Proteomics Databases



Metabolomics Databases

• • X-ray Structural Analysis

  X-ray structural analysis (X-ray crystallography) is a classical physical method that exploits the interaction between X-rays and crystalline materials to determine the three-dimensional structures of molecules. It is a cornerstone technique in structural biology, medicinal chemistry, and materials science. By obtaining high-quality crystals of target molecules and irradiating them with X-rays to collect diffraction patterns, researchers can reconstruct electron density maps and accurately derive atomic....

• • High Throughput Chemical Screening

  High throughput chemical screening refers to a technology that enables the rapid evaluation of the biological activity or chemical properties of large numbers of compounds within a short period. By leveraging automation, miniaturization, and a variety of technical approaches, this method allows researchers to efficiently identify compounds with potential biological relevance. In drug discovery, HTS is extensively utilized during the initial screening phase for targeted therapeutics. With the support of.....

• • Inductively Coupled Plasma

  Inductively coupled plasma (ICP) is a high-temperature plasma generated by exciting an inert gas (usually argon) under a high-frequency electromagnetic field. It is widely utilized in analytical chemistry for elemental excitation, ionization, and detection. Inductively coupled plasma reaches extremely high temperatures (typically between 6000–10000 K), sufficient to excite or ionize most chemical elements, thereby serving as an efficient excitation source for subsequent spectroscopic or mass spectrometric..

• • Single-Cell Bisulfite Sequencing

  Single-cell bisulfite sequencing is a high-throughput sequencing technique designed to assess DNA methylation at single-cell resolution. By integrating bisulfite conversion, single-cell isolation, and high-throughput sequencing, this technology can comprehensively map the genome-wide distribution of 5-methylcytosine (5mC) within individual cells. DNA methylation, a crucial epigenetic regulatory mechanism, plays significant roles in cell fate determination, embryonic development, gene expression regulation..

• • Cell Supernatant Proteomics

  Scope Cell supernatant proteomics enables the systematic identification of secreted proteins present in the extracellular environment. These proteins often play critical roles in intercellular communication, immune responses, and disease progression. Identifying secreted proteins in the cell supernatant provides valuable insights into extracellular signaling pathways and cellular responses under various physiological and pathological conditions.   Quantitative analysis of proteins allows researchers t......

• • Comparison of Peptide Content Determination Methods

  Peptide content determination methods play a critical role in fields such as biomedical research, drug development, and protein engineering. Accurate quantification of peptide content facilitates a deeper understanding of their biological functions, supports dose-response evaluations in pharmacology, and ensures quality control throughout production processes. Currently, several primary peptide content determination methods are widely employed, each with distinct principles, advantages, and limitation......

• • How to Analyze Quantitative Proteomics Data

  To analyze quantitative proteomics data is to engage in a complex, multi-step process that spans from raw data preprocessing to statistical evaluation and biological interpretation. The following outlines a typical analytical workflow:   1. Data Preprocessing Mass spectrometry data processing begins with software tools such as MaxQuant or Proteome Discoverer, which perform critical steps including peak detection, charge state deconvolution, and mass calibration.   Protein identification is conducted b......

• • O-Glycosylation Detection

  O-glycosylation detection is essential for understanding one of the most prevalent forms of post-translational modification (PTM) found in proteins and peptides. O-glycosylation involves the covalent attachment of a sugar moiety—commonly N-acetylgalactosamine (GalNAc), galactose, or xylose—to the hydroxyl group of serine (Ser) or threonine (Thr) residues via an O-glycosidic bond. This modification plays a critical role in regulating protein function, stability, and intercellular communication. Aberran......

• • Methods for Protein Purity Determination

  Methods for protein purity determination must be carefully selected and accurately applied to obtain reliable analytical data. The accuracy of purity assessment has a direct impact on the reproducibility and interpretability of subsequent experiments. Several widely used methods for protein purity determination are outlined below.   SDS-Polyacrylamide Gel Electrophoresis (SDS-PAGE) 1. Principle SDS-PAGE separates proteins in a mixture based on their molecular weight. Proteins are denatured and uniform......

• • Advances in Protein Research: Recombinant Expression, Purification, and Identification

  In the field of biopharmaceuticals, proteins play a critical role in drug development processes. Through protein research, scientists can better understand protein structures and functions, and assess whether a given protein meets specific therapeutic or experimental requirements. Recent studies have reported significant advances in the areas of recombinant protein expression, purification, and identification. This article provides a detailed overview of these developments, with a particular focus on ......