Resources

Proteomics Databases



Metabolomics Databases

• • Amino Acid Analysis Mass Spectrometry

  Amino acid analysis mass spectrometry is an efficient technique for separating, quantifying, and identifying amino acids. Amino acids are the fundamental building blocks of life, involved not only in protein synthesis but also in cellular signal transduction and energy metabolism. Using high-resolution mass spectrometry, this technique can accurately measure the types, concentrations, and distributions of amino acids in a sample, revealing complex metabolic networks and physiological states in organis......

• • ABPP Assay

  The ABPP Assay is a powerful chemical proteomics technique used for the specific labeling, separation, and identification of active enzymes or proteins in complex biological systems. Unlike traditional proteomics methods, the ABPP assay emphasizes not only protein expression levels but also the dynamic detection of protein activity. This technique utilizes small-molecule probes that covalently bind to the active sites of target proteins, enabling highly specific identification of their functional stat......

• • Absolute Quantification Mass Spectrometry

  Absolute quantification mass spectrometry is a method used for the precise measurement of the absolute levels of specific proteins in biological samples, leveraging mass spectrometry technology. Unlike traditional relative quantification techniques such as iTRAQ, TMT, or label-free quantification, this approach provides precise quantification of target proteins by employing standards or isotopically labeled internal standards. This method circumvents potential errors introduced by sample variability, ......

• • LC-MS Protein Identification

  LC-MS protein identification is a technique for the identification and quantification of proteins using liquid chromatography-mass spectrometry (LC-MS). In proteomics research, LC-MS protein identification is invaluable. LC-MS technology integrates the separation ability of liquid chromatography with the sensitivity of mass spectrometry, allowing for the rapid and efficient identification of proteins in complex biological samples. Through LC-MS protein identification, researchers can determine protein......

• • Label-Free Quantification Mass Spectrometry

  Label-free quantification mass spectrometry (LFQ-MS) is an advanced technique for protein quantification that operates without the use of isotopic or chemical labels. Traditional methods of protein quantification often require the addition of labels during sample preparation to distinguish between proteins from different sources during analysis. Label-free quantification mass spectrometry simplifies this by directly analyzing the mass spectrometric data of protein peptides. By comparing the ion intens......

• • Label-Free Quantitative Proteomics in Protein Interactions

  Label-free quantitative proteomics is an effective technique for analyzing and comparing protein expression levels in biological samples without using isotopes or chemical labels. Utilizing high-resolution mass spectrometry, this approach enables the direct acquisition of comprehensive data on thousands of proteins from complex biological samples. Its primary advantage lies in its label-independent sensitivity and accuracy, making it ideal for various biomedical research applications.   In practice, l......

• • Olink Proteomics Panel Selection

  The Olink proteomics panel is a high-throughput, high-sensitivity biomolecular analysis tool that enables the simultaneous analysis of hundreds of proteins in a single run. Selecting the most suitable panel can facilitate efficient data acquisition and interpretation for researchers. This guide provides detailed assistance to help you identify and select the optimal Olink proteomics panel for your research needs.

• • Ion Exchange Chromatography Protein

  Ion Exchange Chromatography Protein Analysis is a commonly employed technique in protein separation and purification. This method capitalizes on differences in the surface charge of protein molecules to separate them using ion exchange chromatographic media. The fundamental principle involves utilizing the electrostatic attraction between charged stationary phases and protein molecules with opposite charges. Renowned for its efficiency and selectivity, this method finds widespread application in bioch......

• • Exosome Proteomics Sample Preparation

  1. Sample Collection: First, samples containing exosomes must be collected. This typically involves gathering from sources such as cell culture media, blood, urine, and cerebrospinal fluid, depending on the research objectives. For instance, tumor cell culture media is often collected for cancer studies. 2. Centrifugation and Ultracentrifugation: Once the samples are collected, exosomes are isolated through a series of centrifugation and ultracentrifugation steps. Initially, low-speed centrifugation........

• • How Many Proteins Can Be Detected by TMT in Proteomics

  Tandem Mass Tag (TMT) is a widely used high-throughput protein quantification technique in proteomics, designed to investigate protein expression differences across various samples. TMT enables the quantitative comparison of corresponding proteins between samples by labeling peptides with specific tags following protein hydrolysis.