Resources
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• Protein Interaction Network Analysis and Interpretation Based on Pull-Down Mass Spectrometry
Pull-Down Mass Spectrometry (PDMS) technology serves as an effective method for analyzing protein interactions, enabling researchers to uncover protein interaction networks. This provides crucial insights for understanding signaling pathways and elucidating disease mechanisms. Principles of Pull-Down Mass Spectrometry Pull-Down Mass Spectrometry is a protein interaction analysis technique based on affinity chromatography. The fundamental principle involves using specific antibodies or affinity agent......
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• Exploring the Application Potential of Pull-Down Assays for Disease-Related Protein Identification
Pull-down assays, a widely used method for analyzing protein-protein interactions, have shown significant potential in disease-related research. Principle and Procedure of Pull-Down Assays Pull-down assays are experimental techniques designed to identify and characterize protein-protein interactions. These assays typically involve the following steps: 1. Antibody Immobilization Specific antibodies are covalently bound to a solid-phase matrix, such as agarose or magnetic beads. 2. Sample Incubati......
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• Protein Pull-Down: A Powerful Tool for Investigating Protein-Protein Interactions
Protein Pull-Down is a highly effective method for capturing target proteins and identifying their interacting partners using specific affinity agents. This technique leverages the specificity of protein-protein interactions to co-capture target proteins alongside their interacting partners through well-defined binding reactions. Technical Principles and Workflow of the Protein Pull-Down Technique The Protein Pull-Down technique comprises four key steps: sample preparation, affinity agent binding, w......
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Peptide fragmentation is a technique that involves breaking down large macromolecular proteins into smaller peptide fragments to facilitate subsequent analysis using mass spectrometry (MS), protein identification, sequence analysis, and functional studies. Protein structure and function are pivotal in influencing the health and disease states of organisms. Thus, precise identification and analysis of protein structural features are crucial. However, due to their complex three-dimensional structures an......
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SILAC mass spectrometry is a high-throughput quantitative analysis method based on mass spectrometry, which uses stable isotope-labeled amino acids to precisely compare protein expression levels across different samples. With the development of mass spectrometry technology, SILAC mass spectrometry has become a core technique in proteomics research, widely applied in various fields such as cancer research, drug screening, cell signaling pathway analysis, and disease mechanism studies. Through this tech......
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• Activity Based Protein Profiling ABPP
Activity based protein profiling ABPP is a powerful technique aimed at understanding protein functions and dynamic cellular processes by specifically labeling and analyzing active proteins in biological samples. This method has broad applications in biological research, drug screening, and exploring disease mechanisms, with significant potential for identifying drug targets, revealing enzyme activities, and elucidating their roles in various diseases. ABPP enables real-time monitoring of protein activ......
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Antibody characterization refers to the in-depth analysis of the biological properties, structural features, affinity, stability, and antigen-binding capabilities of antibodies. As key immune molecules, antibodies can specifically recognize and bind to antigens, thereby performing essential immune functions such as neutralization and pathogen clearance. With the increasing application of antibody drugs and immunotherapies, the demand for antibody characterization technologies has grown significantly, ......
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• Mass Spectrometry Based Proteomics
Mass spectrometry based proteomics enables the efficient and precise analysis of the structure, function, and interactions of thousands of proteins within biological organisms. As the most essential biomolecules in living systems, proteins execute nearly all biological functions both inside and outside the cell. The overarching goal of proteomics is to comprehensively catalog the types, quantities, and dynamic variations of all proteins present in a specific organism, cell, or tissue. By employing mas......
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Protein characterization is a crucial step in the study of protein structure, function, and its roles in biological systems. Through comprehensive protein characterization, scientists can determine important features such as amino acid sequences, three-dimensional structures, functional domains, and post-translational modifications. These insights are essential for understanding fundamental biological processes and their implications in health and disease. In fields such as biomedicine, drug developme......
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SDS PAGE Gel Analysis is a widely used protein analysis technique based on the principle of separation using an electric field and polyacrylamide gel. This technique involves separating proteins in polyacrylamide gel via electrophoresis, utilizing differences in protein molecular sizes for the analysis. Sodium dodecyl sulfate (SDS), an anionic surfactant, binds to protein molecules, denaturing them by disrupting their three-dimensional structure and converting them into linear forms, while imparting a......
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