Resources

Proteomics Databases



Metabolomics Databases

• • Total Amino Acid Analysis

  Total amino acid analysis involves the comprehensive quantitative and qualitative assessment of all amino acids present in biological or chemical samples. By comparing retention times of sample amino acids to those of standards, the identity of amino acids can be established. A match in retention times indicates the presence of a specific amino acid in the sample. Quantification is achieved through standard curve methodology, where standard curves for each amino acid are constructed. By measuring the ......

• • Antibody De Novo Sequencing: Advances in Mass Spectrometry

  Antibody de novo sequencing is the process of determining the amino acid sequence of an antibody purely through the analysis of the antibody protein itself, without relying on known genetic sequence templates. Mass spectrometry is central to this process. Advances in mass spectrometry, such as the ethcd fragmentation mode in eclipse mass spectrometry, enable the differentiation of leucine (Leu) and isoleucine (Ile) through neutral losses of 43Da and 29Da from their side chains, respectively. This deve......

• • Biomarker Discovery and Validation

  Biomarker discovery and validation represent key research directions in modern medicine and biology. Biomarkers are indicators detectable through biological techniques, reflecting the presence or change of biological or pathological states. These indicators can include a range of biomolecules such as genes, proteins, and metabolites, as well as physiological and biochemical parameters. The discovery and validation of biomarkers are crucial for early disease diagnosis, therapeutic monitoring, and the d......

• • Protein Mass Spectrometry Service: Precision Protein Analysis

  Protein mass spectrometry services are specialized offerings that utilize mass spectrometry technology for the analysis of proteins. This technology, known for its high sensitivity and resolution, enables the precise identification and quantification of proteins. These services are primarily employed to investigate the structure, function, and interactions of proteins. The fundamental principle involves converting protein molecules into charged ions, which are then analyzed based on their mass-to-char......

• • Amino Acids HPLC

  Amino acids HPLC analysis is a rapid, efficient, and sensitive technique for analyzing the amino acid composition of complex samples. Due to the wide variations in polarity and molecular weight among amino acids, traditional analytical methods face challenges in effectively separating them. By selecting appropriate stationary and mobile phases, HPLC enables the precise separation and detection of different amino acids. High-performance liquid chromatography (HPLC) analysis of amino acids relies on dif......

• • iTRAQ/TMT: Isotope Labeling and Tandem Mass Spectrometry Quantification

  iTRAQ/TMT (isotope labeling and tandem mass spectrometry quantification analysis) rely on the principle of using isotope tags to label proteins, enabling the simultaneous comparison of multiple samples in mass spectrometry analysis. iTRAQ (Isobaric Tags for Relative and Absolute Quantitation) and TMT (Tandem Mass Tag) are widely used isotope labeling and quantification techniques in tandem mass spectrometry. These methods allow researchers to conduct both relative and absolute quantification of protei......

• • Amino Acid Profile Analysis

  Amino acid profile analysis is a technique used to assess the types, concentrations, and proportions of various amino acids in biological samples, such as blood, tissues, and cell extracts, as well as in food samples. As fundamental building blocks of proteins, amino acids directly influence protein structure and function. This analysis enables researchers to determine both the relative and absolute quantities of amino acids present in a sample, unveiling potential biological functions and application......

• • Co-IP Protein Interaction

  Co-immunoprecipitation (Co-IP) is a classical biochemical technique used to study Co-IP protein interaction by isolating and detecting protein complexes from cell extracts. Using specific antibodies to target proteins, Co-IP enables researchers to analyze protein interactions and their roles in biological processes. This technique is widely applied in areas such as cell signaling, gene expression regulation, and cell cycle control, as it helps uncover complex protein networks and signaling pathways.  ......

• • Applications and Prospects of C-Terminal Sequencing in Proteomics

  C-terminal sequencing, a proteomics analytical method, specifically targets the identification of protein C-terminal sequences. The protein C-terminus is critical for its function, stability, degradation, and interactions. Therefore, obtaining an in-depth understanding of protein C-terminal sequences holds significant value in biological research. Recently, with continuous advancements in mass spectrometry (MS), the technology has made substantial progress and is widely applied in various proteomics f......

• • The Evolution of C-Terminal Sequencing: From Traditional to Modern Techniques

  C-terminal sequencing, the methodology for determining the sequences at protein C-termini (carboxyl termini), is a critical technique in proteomics. Compared to N-terminal sequencing, the analysis of C-terminal sequences has encountered numerous challenges, making its development a reflection of both technological advancements and the evolution of methods in protein chemistry research. From traditional chemical degradation to modern high-resolution mass spectrometry, this technology has undergone sign......