Resources

Proteomics Databases



Metabolomics Databases

• • Plant Mass Spectrometry Detects Protein Interactions

  Using mass spectrometry to study protein-protein interactions in plants represents a significant direction in contemporary plant proteomics research. By analyzing protein interaction networks, scientists gain deeper insights into physiological processes such as plant growth and development, environmental adaptation, and disease defense. This approach not only identifies direct interactions between specific proteins but also elucidates the composition of protein complexes and their physiological roles in....

• • Mass Spectrometry Combined with Protein

  Mass spectrometry combined with proteomics represents a powerful analytical tool for the identification and quantification of proteins within cells. This process can generally be divided into the following steps:

• • Analysis of Sample Volume Required for Exosome Protein Identification

  Exosomes are vesicles released by cells into the external environment that contain proteins, RNA, and other molecules, which reflect the biological state of their source cells. Identifying exosomal proteins through mass spectrometry typically requires only small sample amounts, though the precise quantity depends on several factors, such as the sensitivity of the mass spectrometer, the purity of the sample, and the desired data depth and coverage.

• • Peptide Detection in Serum

  The detection of peptides in serum is widely used in disease diagnosis, therapeutic monitoring, and biomedical research. Peptides, as a class of small protein fragments with specific biological activities, play crucial roles in many physiological and pathological processes.

• • Target Protein Mass Spectrometry

  Mass spectrometry (MS) is a powerful analytical technique widely used for the identification and quantification of proteins in various samples. This technology plays a critical role in biological research, biomedical studies, and drug development, particularly within the field of proteomics. MS can provide precise molecular weights, structural details, and information on post-translational modifications, such as phosphorylation, glycosylation, or ubiquitination, of proteins. The typical workflow for mass...

• • Immunoprecipitation Mass Spectrometry Analysis

  Immunoprecipitation-Mass Spectrometry (IP-MS) is a method that combines immunoprecipitation (IP) techniques with mass spectrometry (MS) technology to study protein interactions and the composition of protein complexes. This technique can identify proteins that interact with specific proteins or protein complexes, thus providing insights into protein functions and intracellular signaling pathways.

• • Detect Protein Ubiquitination by Immunoprecipitation

  Immunoprecipitation (IP) is a valuable molecular biology technique employed to isolate proteins or protein complexes from complex cellular lysates through the use of specific antibodies. This method is extensively utilized to investigate protein-protein interactions, protein modification states (such as phosphorylation and ubiquitination), and the composition of protein complexes. Ubiquitination, an essential post-translational modification, involves the covalent attachment of ubiquitin, a small regulatory

• • In Gel Digestion Mass Spectrometry

  In gel digestion mass spectrometry combines polyacrylamide gel electrophoresis (PAGE) with mass spectrometry analysis, enabling in-depth characterization of complex protein samples. Gel electrophoresis allows the separation of complex protein mixtures into individual bands, which are then digested in the gel. The resulting peptides are analyzed by mass spectrometry, facilitating both protein identification and quantification. Since its development in the late 20th century, in gel digestion mass spectr......

• • Host Cell Protein Quantification

  Host cell protein quantification (HCP quantification) is a critical method used to detect and measure residual proteins originating from host cells during the biopharmaceutical manufacturing process. These host cell proteins are non-target proteins expressed and released by production host cells, other than the target therapeutic protein, and they are typically considered unavoidable impurities in drug production. The presence of such proteins may adversely affect the safety, purity, and stability of ......

• • Quantification of Antibodies

  Quantification of Antibodies refers to the process of measuring the concentration of antibodies using various biochemical and molecular biology techniques. Antibodies are crucial components of the immune system, tasked with identifying and neutralizing foreign substances such as bacteria and viruses. In research, clinical diagnostics, and therapeutic settings, the quantification of antibodies has diverse applications. For example, in biopharmaceutical research, this technique is used to evaluate vacci......