Resources

Proteomics Databases



Metabolomics Databases

• • Overview: N-terminus and C-terminus

  Proteins execute their functions based on highly defined three-dimensional structures, the formation of which originates from the linear sequence of amino acids. Within this polypeptide chain, the two termini—the amino terminus (N-terminus) and the carboxyl terminus (C-terminus)—represent not only the start and end of protein biosynthesis, but also serve as critical regulatory sites influencing protein localization, stability, and degradation. A comprehensive understanding of the structural and functional..

• • How to Know C and N Terminus in a Primary Sequence

  The primary sequence of a protein is a linear chain of amino acids covalently linked by peptide bonds in a defined order. At the two ends of this polypeptide chain lie the N-terminus (amino terminus, N-terminal) and the C-terminus (carboxyl terminus, C-terminal), respectively. Accurate identification of the N-terminus and C-terminus is essential for protein characterization, functional analysis, studies of post-translational modifications (PTMs), and quality control in biopharmaceutical development.

• • What Is Edman Sequencing? A Key Technology in Protein Sequencing Explained

  Proteins play essential roles in virtually all biological processes, and their primary structure—the linear sequence of amino acids—determines their function. To investigate protein structure and function, it is essential to first understand their amino acid composition and sequence. As such, protein sequencing represents a critical step in modern life science research and biopharmaceutical development. Among various sequencing methods, Edman sequencing is widely recognized as a classical approach for......

• • What Is the Difference between Targeted and Untargeted Proteomics

  Targeted proteomics and untargeted proteomics represent two widely used but fundamentally different strategies in proteomics research. They differ significantly in research objectives, methodological approaches, data output, and appropriate application scenarios. A clear understanding of their distinctions is crucial for researchers to design experiments effectively, interpret results accurately, and select suitable analytical platforms. 1. Different Research Objectives: Quantitative Accuracy........

• • Targeted Proteomics Workflow/How Does Targeted Proteomics Work?

  In contemporary proteomics research, targeted proteomics has emerged as a pivotal analytical methodology in areas such as biomarker validation, investigation of drug mechanisms of action, and clinical companion diagnostics, owing to its superior quantification precision and high reproducibility. Unlike discovery proteomics, which emphasizes broad-scale exploration, targeted proteomics focuses on deep validation and precise quantification, thereby imposing more stringent requirements on both workflow .......

• • Optimizing PRM Quantitative Proteomics for High-Sensitivity Protein Detection

  Accurate detection of low-abundance proteins remains a fundamental challenge in proteomics, particularly in areas such as early disease screening, biomarker validation, and drug target discovery. Parallel Reaction Monitoring (PRM) has emerged as a leading approach in targeted proteomics due to its superior quantitative accuracy and analytical specificity. Through systematic optimization of PRM strategies, researchers are steadily advancing the sensitivity of detection, enabling reliable quantification of...

• • 5 Strategies to Address Challenges in Targeted Proteomics

  Targeted proteomics has become increasingly prominent in recent years in the fields of biomarker validation, investigation of disease mechanisms, and translational medicine. In contrast to discovery-based approaches, targeted methods prioritize quantitative accuracy, detection sensitivity, and reproducibility. However, a range of technical obstacles persist in practical applications, including the detection of low-abundance proteins, lack of standardized methodologies, and variability in data ........

• • What is Targeted Quantitative Proteomics

  Targeted Quantitative Proteomics is an analytical approach in proteomics characterized by high sensitivity and specificity. It is widely applied in biomarker validation, elucidation of disease mechanisms, pharmacodynamic assessment, and quality control. Compared with discovery-based proteomics, targeted quantification emphasizes precise and highly reproducible quantitative analysis of predefined target proteins. This article systematically outlines the fundamental principles, mainstream technical ..........

• • How to Determine Protein Full-Length Sequence

  Determining the protein full-length sequence is a foundational task in protein research, particularly crucial in studies involving novel protein functions, structural analysis, antibody development, and recombinant expression. But how can researchers obtain the complete amino acid sequence of a protein? This article provides a technical overview and practical strategies based on current mainstream approaches and recent advances. What Is a Protein Full-Length Sequence? A protein full-length sequence refers..

• • Full-Length Sequencing Strategies for Protein Isoform Characterization

  From subtle structural differences to functional assessment, how can protein full-length sequencing uncover the “hidden information” embedded within isoforms? In the development of macromolecular biopharmaceuticals—such as protein-based therapeutics, recombinant enzymes, and engineered antibodies—the presence of protein isoforms often represents a double-edged sword: On one hand, naturally occurring isoforms introduce structural diversity and serve as a foundation for functional regulation; On the other....