Resources
Proteomics Databases
Metabolomics Databases
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Hybridoma sequencing involves the genomic sequencing of cells generated through hybridoma technology, which is crucial for exploring the genomic composition of hybridoma cells. These cells are produced in laboratories by fusing antibody-producing B lymphocytes with immortalized myeloma cells, resulting in cell lines capable of both indefinite proliferation and antibody secretion. This technique underpins the production of monoclonal antibodies, essential for creating cells that retain the specificity ......
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• Amino Acid Analysis LC-MS Plasma
Amino acid analysis LC-MS plasma utilizes the combined strengths of liquid chromatography and mass spectrometry for precise detection and quantification of plasma amino acids. This technique enables accurate characterization of amino acid composition, concentration, and potential modifications. As essential metabolic components, plasma amino acids play critical roles in protein synthesis, energy metabolism, and neurotransmission. Measuring plasma amino acid levels with high accuracy is crucial for ass......
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Lipidomics pathway analysis is a method for studying the intricate processes of lipid synthesis, degradation, and transformation in living organisms. Lipids, a diverse group of organic compounds including fats, phospholipids, and sterols, are ubiquitous in biological systems. This analysis focuses on lipid molecules and their metabolic pathways, aiming to elucidate their roles and mechanisms in biological functions. By employing lipidomics pathway analysis, researchers can gain deeper insights into di......
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Glycopeptide mapping is an analytical technique used to separate, identify, and analyze glycopeptides derived from glycoproteins following enzymatic or chemical cleavage. This method provides detailed information about glycopeptide structures, glycosylation sites, and glycan composition. Glycopeptides are macromolecules formed when sugar chains covalently bond to protein amino acid residues. Glycosylation plays a critical role in various biological processes, including cellular signal transduction, im......
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Mass spec phosphorylation analysis refers to the study of protein phosphorylation using advanced mass spectrometry techniques. The process begins with protein extraction from biological samples, such as cells or tissues. Due to the low relative abundance of phosphorylated proteins and the instability of phosphate groups, specialized enrichment methods, such as immunoprecipitation and chemical affinity chromatography, are commonly employed to isolate phosphorylated proteins or peptides. Once enriched......
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• Absolute Protein Quantification
Absolute protein quantification involves determining the precise amount, either in mass or molarity, of proteins within a biological sample. Unlike relative protein quantification, which compares protein levels across different samples, absolute protein quantification provides specific values for protein concentrations. This process typically combines mass spectrometry and isotope labeling techniques to facilitate precise quantification by comparing standards with samples. For instance, in cancer rese......
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The protein-protein interactome represents the intricate network of interactions formed by proteins both inside and outside cells. Proteins are key players in biological processes, forming the core of many life functions through their interactions. These interactions specifically influence processes such as signal transduction, metabolic pathways, cell cycle regulation, gene expression, and immune responses. Research on the protein-protein interactome not only aids in understanding the fundamental mec......
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Oligosaccharide profiling is aimed at thoroughly analyzing the complex structures of oligosaccharides to elucidate their biological functions and roles. Oligosaccharides, composed of a limited number of monosaccharides linked by glycosidic bonds, are prevalent on the surfaces of animal and plant cells and are involved in various biological processes such as cell recognition, immune response, and signal transduction. By comprehensively examining their structures, compositions, and modifications, oligos......
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• Protein Sequencing Technology
Protein sequencing technology is fundamental to determining the amino acid sequences of proteins, providing critical insights into their structure-function relationships. This technology is widely applied in medicine, drug discovery, and biotechnology. In disease research, protein sequencing aids in identifying disease-associated protein variants, supporting early diagnosis and personalized treatment. Furthermore, sequencing specific proteins enables scientists to design engineered proteins with tailo......
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• C-Terminal Protein Sequencing in Biopharmaceuticals
C-Terminal protein sequencing analysis is a technique that precisely analyzes the carboxyl-terminal sequence of proteins or polypeptides. This technique is crucial for identifying C-terminal amino acid sequences, detecting modifications at the C-terminal, and confirming the completeness of protein processing. The C-terminal sequences play significant roles in various biological processes, including the regulation of protein stability, functional activity, interactions, and signal transduction. Moreove......
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