Resources

Proteomics Databases



Metabolomics Databases

• • LC-MS/MS Based C-Terminal Sequencing: Technical Advances and Challenges

  One of the central objectives of proteomics is to elucidate the complete amino acid sequences of proteins. In this context, C-terminal sequencing has attracted increasing attention in recent years due to its critical role in understanding protein modifications, degradation pathways, and biological functions. The rapid advancement of liquid chromatography–tandem mass spectrometry (LC-MS/MS) has offered powerful analytical capabilities for C-terminal sequencing. However, challenges remain due to the lim......

• • How to Improve C-Terminal Sequencing Sensitivity and Accuracy with LC-MS/MS?

  In C-terminal sequencing with LC-MS/MS, improving sensitivity and accuracy requires a systematic and integrated approach involving sample preparation, optimization of instrumental settings, and data analysis strategies. The following are major strategies for enhancement:   Selection of Appropriate Proteases and Sample Preparation Strategies The key to successful C-terminal sequencing lies in achieving high specificity and efficiency in generating C-terminal peptides. While conventional proteases such ......

• • BiFC Analysis

  BiFC analysis is a technique used to investigate protein-protein interactions. This method leverages the reconstitution of fluorescent protein fragments, where a fluorescent protein (e.g., yellow fluorescent protein (YFP) or enhanced green fluorescent protein (EGFP)) is divided into two non-fluorescent fragments, each fused to a different protein of interest. When these proteins interact within cells or in vitro, the protein fragments come into proximity and reassemble, restoring the fluorescence sign......

• • Antibody Screening and Identification

  Antibody screening and identification are critical steps in life sciences research and the development of biopharmaceuticals. Antibodies play a vital role in the immune system's ability to recognize and neutralize pathogens, and therefore have broad applications in disease diagnosis, treatment, and research. This process involves selecting antibodies from a pool of candidates that possess specific target-binding capabilities and the required characteristics. Typically, this process encompasses the con......

• • Human Protein Interactome

  The human protein interactome is a vital area of contemporary life sciences research, representing the complex network formed by protein interactions both inside and outside of cells. As essential molecular participants in nearly all biological processes-such as signal transduction, metabolic regulation, cell cycle control, and immune response-proteins typically do not function in isolation. Instead, they form intricate networks through interactions, enabling specific biological functions. Therefore, ......

• • End-Group Analysis of Peptides

  End-group analysis of peptides is a technique in proteomics research used to study and identify the start and end positions of amino acid sequences in proteins or peptide chains. This analysis not only aids scientists in understanding protein structure and function but also reveals the metabolic pathways and mechanisms of action within organisms. The peptide end groups-typically referring to the N-terminus (amino terminus) and the C-terminus (carboxyl terminus)-are critical for protein bioactivity and......

• • End-Group Analysis of Protein

  End-group analysis of protein is a technique in proteomics research primarily used to determine the amino acid sequences at both termini of protein molecules. Typically, protein ends include the N-terminus (amino terminus) and the C-terminus (carboxyl terminus). This analysis not only provides structural information but also elucidates various biological functions. For example, the N-terminus is closely linked to signal peptide recognition, protein localization, and cellular half-life, while the C-ter......

• • Detecting Phosphorylated Proteins

  Detecting phosphorylated proteins refers to the process of identifying and analyzing the presence and localization of phosphate groups on protein molecules using specific experimental techniques and methods. Phosphorylation, the most common type of post-translational modification (PTM) in proteins, regulates protein activity, localization, and interactions by adding phosphate groups to serine, threonine, or tyrosine residues. It plays a critical role in biological processes such as cell signaling, met......

• • Capillary Electrophoresis Protein Analysis

  Capillary electrophoresis protein analysis is a highly effective separation technique that leverages narrow-diameter capillaries and high-voltage electric fields to facilitate the movement of protein molecules in an electrolyte solution, leading to efficient separation and analysis. This method is extensively employed in biochemical and molecular biology research for the purposes of protein separation, purification, characterization, and quantitative analysis. The principle underlying this technique d......

• • Characterization of Biologics and Biosimilars

  Characterization of biologics and biosimilars is a critical step in evaluating their quality, safety, and efficacy. Biologics refer to therapeutic proteins, antibodies, vaccines, cell therapy products, and similar agents produced by living cells, and are widely used in the treatment of cancer, autoimmune disorders, and rare diseases. Owing to the complex structure of biologics, their characterization involves a variety of analytical techniques and rigorous quality control methods to ensure drug safety......