Resources
Proteomics Databases
Metabolomics Databases
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• Proteomics Bottom-up & Top-down
Proteomics bottom-up & top-down approaches are two fundamental strategies in modern life sciences, primarily used to investigate the composition and functional properties of proteins in biological samples. Bottom-up proteomics involves enzymatic digestion of proteins into peptides, followed by mass spectrometry (MS) analysis to reconstruct protein-level information. In contrast, top-down proteomics directly analyzes intact proteins via MS without enzymatic digestion, thereby preserving post-translatio......
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• Proteome Analysis by Mass Spectrometry
Proteome analysis by mass spectrometry is a powerful technique for investigating the composition, structure, function, and interactions of proteins in biological samples. As essential biomolecules, proteins participate in nearly all biological processes, including cellular architecture, signal transduction, metabolic regulation, and immune responses. By fractionating complex protein mixtures and performing qualitative and quantitative analyses, this approach enables a comprehensive understanding of ce......
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Protein stability analysis is a scientific approach used to evaluate a protein’s ability to retain its structural integrity and functionality under varying environmental conditions. As essential biomolecules, proteins not only provide structural support to cells and tissues but also function as enzymes, signaling molecules, and transport carriers in biochemical processes. Protein stability analysis has broad applications across drug development, industrial enzyme engineering, food science, and fundame......
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MS/MS Proteomics, or tandem mass spectrometry proteomics, is an advanced analytical technique used to explore the protein composition and structure of biological samples in depth. By fragmenting proteins into peptide sequences, mass spectrometry enables the identification and quantification of proteins. This method is indispensable in biological and medical research because proteins are integral to cellular functions. Understanding how protein expression changes under various biological states and con......
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• Flow Cytometry Protein Quantification
Flow cytometry protein quantification is a high-throughput analytical technique grounded in flow cytometry, enabling precise measurement of target protein expression at the single-cell level. This method uses fluorescently labeled antibodies that bind specifically to target proteins, with a flow cytometer detecting the intensity of the fluorescence signals to quantify protein expression within a cell population. Unlike traditional protein quantification methods such as Western blot or ELISA, flow cyto......
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Global proteomic analysis is a proteomics approach that systematically investigates all proteins within an organism, focusing on their composition and changes in expression. Unlike traditional methods that analyze one or a few proteins, global proteomics provides a comprehensive view of proteomic features under varying physiological or pathological conditions. This approach aids researchers in understanding complex cellular functions, signaling pathways, and protein interactions. The central aspect of......
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ESI proteomics is a method in proteomics research that leverages ESI technology. This technique involves using a high-voltage electric field to transform protein solutions into charged microdroplets. These droplets convert into gas-phase ions upon desolvation, facilitating efficient ionization and mass spectrometric analysis of proteins and peptides. As a "soft ionization" technology, ESI excels in preserving the structural integrity of macromolecules like proteins, while offering high sensitivity in ......
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Environmental proteomics is an emerging interdisciplinary field that employs high-throughput techniques, such as mass spectrometry, to study the composition, function, and dynamic changes of proteins in environmental samples. This field aims to elucidate the biomolecular response mechanisms within environmental systems and their ecological and health implications. Unlike traditional genomic or metabolomic studies, environmental proteomics directly focuses on proteins, which are the functional expressi......
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Gel-based proteomics is a key method in proteomics research that employs gel electrophoresis for protein separation and analysis. This technique separates proteins based on molecular weight, isoelectric point, or charge, and integrates methods such as staining and mass spectrometry for the qualitative and quantitative analysis of proteins. One of the strengths of gel-based proteomics is its ability to visually represent the complexity of proteomes and highlight differences in protein expression throug......
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DIGE analysis is a powerful technique for studying variations in protein expression, based on the principles of traditional two-dimensional gel electrophoresis (2-DE). Unlike traditional methods, DIGE enhances the accuracy and efficiency of quantitative and differential protein analysis by using fluorescent dyes to label samples. This approach labels samples with distinct colors, allowing for the simultaneous separation and comparison of multiple samples on a single gel, thereby minimizing data bias f......
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