Resources

Proteomics Databases



Metabolomics Databases

• • Mechanism of Peptide Sequencing

  Peptide sequencing is a crucial technique in protein research, allowing scientists to understand the structure and function of proteins by determining the order of amino acids in a peptide chain.

• • Workflow of Peptide Sequencing

  Peptide sequencing is an essential method for studying the structure and function of proteins. By determining the sequence of amino acids in a peptide chain, it aids scientists in understanding the biological functions of proteins and their roles within organisms. The primary steps of peptide sequencing include sample preparation, peptide generation, peptide separation, mass spectrometry analysis, data processing, and result interpretation.

• • The Principle of Peptide Sequencing

  Peptide sequencing involves determining the primary structure of a protein by analyzing the amino acid sequence of peptide fragments. Understanding the amino acid sequence of proteins is crucial for studying their function, structure, and interactions. The development of peptide sequencing technology has advanced proteomics research, enabling scientists to gain deeper insights into the mechanisms of biomolecules.

• • Advantages and Disadvantages of Peptide Sequencing

  Peptide sequencing involves determining the sequence of amino acids in a peptide chain to elucidate the structure and function of proteins. As proteomics research advances, peptide sequencing plays a crucial role in biomedical research, drug development, and disease diagnosis. However, this technology also has certain limitations in its application.

• • Antibody Drug Glycosylation Analysis

  Antibody drug glycosylation analysis is a critical bio-pharmaceutical analysis method, specifically used to evaluate and monitor the glycan structures on antibody drugs. Glycosylation analysis is crucial for ensuring the safety, efficacy, and consistency of drugs, as the structure and composition of glycans directly influence the bioactivity and pharmacokinetic properties of antibody drugs.

• • Methods for Detecting Protein Phosphorylation

  Detecting protein phosphorylation is a critical task in biological and biochemical research, as phosphorylation is one of the key processes in cell signaling. Here are some commonly used methods for detecting protein phosphorylation.   1. Immunoblotting (Western Blotting) This is one of the most common methods for detecting protein phosphorylation. It involves extracting proteins from cells or tissues and then separating them by SDS-PAGE electrophoresis.

• • Phosphoprotein Sequencing

  Phosphoprotein sequencing is a technique used to analyze protein phosphorylation modifications. Phosphorylation is an important post-translational protein modification that affects the function, activity, stability, and interactions of proteins by adding phosphate groups to specific amino acid residues (usually serine, threonine, or tyrosine) on proteins.

• • Determination of Monosaccharide Content and Types in Recombinant Protein Vaccines

  Recombinant protein vaccine is a type of vaccine that does not contain complete pathogens and is prepared from specific protein antigens produced by heterologous expression systems. Due to their advantages of good safety, strong stability, and relatively low cost, recombinant protein vaccines have been widely favored by researchers in recent years.

• • Deacetylation Detection

  Deacetylation refers to the process of removing acetyl groups from specific lysine residues of proteins. This modification is crucial for regulating the function of proteins and intracellular signal transduction. This process is mainly catalyzed by histone deacetylases (HDACs) and has a significant impact on biological processes such as gene expression regulation, protein stability, and function.

• • How Can Protein Mass Spectrometry Be Used for Protein Sequencing?

  Proteins are vital components within living organisms, playing a key role in the structure and function of cells. Understanding the sequence of proteins is essential for comprehending their function and studying biological processes. Proteomics is a powerful technology that can be used to determine protein sequences.