Resources

Proteomics Databases



Metabolomics Databases

• • LC-MS/MS Peptide Mass Spectrometry Analysis of Ubiquitination Sites

  Ubiquitination is a prevalent post-translational modification instrumental in regulating numerous cellular processes, including protein degradation, signal transduction, and protein subcellular localization. Consequently, precise identification and localization of ubiquitination sites are of great biological significance. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is a pivotal analytical technique used in the identification of ubiquitination sites.

• • Visualization of Glycosylation Sites Detection

  Glycosylation is a crucial post-translational modification that occurs widely in living organisms. This process affects a diverse range of proteins and lipids, enabling various physiological functions. However, the precise detection and localization of glycosylation sites remain a significant challenge in molecular and cellular biology due to technical limitations. This paper reviews recent advancements in glycosylation site visualization techniques.

• • Phosphorylation Site and Abundance Detection

  Phosphorylation is a critical post-translational modification (PTM) of proteins, integral to several biological processes such as signal transduction, cell division, and metabolism. This process involves the addition of a phosphate group to specific amino acid residues, typically serine (S), threonine (T), and tyrosine (Y).

• • Detection of Protein Carboxylation Modification

  Protein carboxylation is a prevalent post-translational modification that involves the addition of a carboxyl (-COOH) group to specific amino acid residues in proteins. This modification alters the protein’s charge distribution, which in turn affects its structural conformation and functional properties. Given its critical role in protein stability and function, the accurate detection of carboxylation is essential for studying protein function, elucidating disease mechanisms, and facilitating drug..........

• • Chemical Proteomics Protocol

  Chemical proteomics is a scientific methodology designed to investigate interactions between proteins and small molecules within biological systems. By employing chemical techniques, researchers can perform both qualitative and quantitative analyses of specific proteins, thereby elucidating their functions and regulatory mechanisms. The following sections outline the fundamental steps involved in chemical proteomics analysis.

• • Differential Proteomics Analysis of Mice

  Mouse models are instrumental in predicting and elucidating the pathophysiological mechanisms of human diseases. Proteomics, which involves the large-scale analysis of proteins to understand their structure, function, and interactions, is a pivotal approach in contemporary biomedical research. Differential proteomics analysis, a core component of proteomics, seeks to identify changes in protein abundance across different samples or experimental conditions.

• • Differential Expression Peptide Screening in Proteomics

  Proteomics is a scientific field focused on comprehensively studying the diverse dimensions of proteins, including their expression levels, interactions, modifications, functions, and roles in cellular processes. Advances in proteomics methodologies and applications have positioned it as a crucial tool in biomedical research. Nonetheless, the precise and effective identification of differentially expressed protein peptides remains a significant challenge.

• • PRM LC-MS Targeted Proteomics

  Proteomics is an essential approach in biological research for exploring protein functions and structures. Recently, PRM (Parallel Reaction Monitoring) coupled with LCMS (Liquid Chromatography-Mass Spectrometry) has emerged as a highly precise method for targeted protein analysis, enabling quantitative measurements of specific proteins within the proteome.

• • Differential Protein Analysis and Annotation in Proteomics

  Proteomics is the study of the large-scale expression, structure, and function of proteins. One of its crucial steps is differential protein analysis, which involves collecting protein samples, separating and identifying proteins, and performing quantitative and qualitative analyses. Finally, protein annotation is conducted to gather functional insights.

• • Targeted Quantitative Proteomics SRM

  Selected Reaction Monitoring (SRM) is a widely applied technique in quantitative proteomics. Owing to its exceptional sensitivity and quantitative accuracy, SRM is indispensable in various research domains. It is primarily utilized for the quantitative measurement and validation of specific proteins. The operating principle of SRM is based on detecting and quantifying target analytes via predefined target ions.