Resources

Proteomics Databases



Metabolomics Databases

• • Residual Protein Analysis

  Residual protein analysis is a technique employed to detect and quantify trace proteins in biological samples and processed products, significantly impacting the biopharmaceutical and food processing sectors. In the biopharmaceutical industry, ensuring that drug product impurities remain within safe limits is crucial for guaranteeing safety and efficacy. Similarly, the food processing industry must prevent foreign proteins introduced during production from posing allergenic or health risks to consumer......

• • Secretome Proteomics

  Secretome proteomics is a branch of modern biotechnology research dedicated to investigating the composition and function of proteins secreted by cells. By examining these proteins in the extracellular environment, it elucidates the interactions between cells and their surroundings. Secreted proteins are vital in processes such as development, immune responses, signal transduction, and disease progression. For instance, cytokines, hormones, and growth factors, as secreted proteins, are integral to imm......

• • Tandem Mass Spectrometry Protein Identification

  Tandem mass spectrometry protein identification is an essential technique in modern proteomics research, leveraging the strengths of mass spectrometry and protein chemistry to identify and quantify proteins within complex biological samples. Mass spectrometry analyzes the mass-to-charge ratio (m/z) of molecular ions to gather molecular information, while tandem mass spectrometry enhances this by providing detailed molecular structure insights through multi-stage analysis. In protein identification, ta......

• • Qualitative Analysis of Amino Acids and Proteins

  Qualitative analysis of amino acids and proteins is extensively applied in proteomics, metabolomics, and biomedical research. As fundamental building blocks of proteins, amino acids play crucial roles, with proteins serving as primary agents in virtually all biological processes. Proteins are indispensable in contexts ranging from cellular architecture and molecular signal transduction to enzymatic activity and immune response. The primary objective of qualitative analysis is to identify and character......

• • Shotgun Protein Identification

  Shotgun protein identification is a proteomics technique fundamentally reliant on mass spectrometry. It provides profound biological insights by thoroughly identifying and analyzing complex protein mixtures. Unlike traditional protein identification techniques, Shotgun does not depend on the prior separation and purification of proteins. Instead, it directly examines peptide mixtures generated from enzymatic digestion of protein samples. The methodology is characterized by its high throughput and sens......

• • SILAC-Based Mass Spectrometry

  SILAC-based mass spectrometry is a powerful tool for quantitative proteomics. SILAC involves the endogenous labeling of proteins by introducing stable isotope-labeled amino acids, such as ^13C or ^15N labeled lysine and arginine, into cell cultures. This results in proteins that exhibit specific isotope labels, which can be detected as mass differences in mass spectrometry analysis. SILAC-based mass spectrometry is primarily used for the relative quantification of proteins, playing a crucial role in i......

• • Quantitative Analysis of Newly Synthesized Proteins

  Quantitative analysis of newly synthesized proteins is a powerful approach for measuring and evaluating the expression dynamics of nascent proteins within cells. This technique is essential in proteomics research, offering critical insights into protein synthesis rates, turnover mechanisms, and cellular regulatory pathways. By quantifying newly synthesized proteins, researchers can dissect the processes of protein production and degradation, elucidating cellular responses under various physiological a......

• • Quantitative and Qualitative Analysis of Proteins

  Quantitative and qualitative analysis of proteins is fundamental to understanding biological functions and mechanisms. As key regulators of cellular processes, proteins undergo changes in abundance and structural modifications that directly influence physiological and pathological states. Quantitative analysis primarily focuses on determining protein concentration, enabling researchers to investigate expression variations under different conditions. For instance, comparing protein concentrations betwe......

• • Qualitative Mass Spectrometry

  Qualitative mass spectrometry is an advanced analytical method used for the qualitative characterization of proteins within samples by employing mass spectrometry. As a robust tool, mass spectrometry allows for the identification and characterization of proteins present in complex mixtures. By measuring the mass-to-charge ratio (m/z) of protein molecules and integrating bioinformatics analysis, this approach can elucidate both the protein composition and structural details within a sample. The scope o......

• • PTM Mass Spec

  PTM mass spec is an advanced technique used to examine the modifications proteins undergo after translation. PTMs are chemical changes occurring on specific amino acids during or after protein synthesis, through enzymatic or non-enzymatic processes. These modifications are vital for regulating protein function, stability, and cell signaling. PTMs include phosphorylation, acetylation, methylation, glycosylation, ubiquitination, and carboxylation, among others. By enhancing chemical diversity, PTMs modu......