Resources
Proteomics Databases
Metabolomics Databases
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• Workflow of AQUA in Quantitative Proteomics
AQUA (Absolute Quantification) is an isotope-labeled mass spectrometry-based technique used for the precise absolute quantification of specific peptides from target proteins. By incorporating known amounts of synthetic peptides as internal standards, AQUA provides absolute quantification of target peptides in a sample. The workflow of AQUA involves multiple critical steps, ranging from sample preparation to data processing and analysis, which ensures accuracy and reproducibility.
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• Advantages and Disadvantages of AQUA Technique
Absolute quantification analysis (AQUA) is a mass spectrometry-based technique used for the absolute quantification of proteins. It has become a vital tool in proteomics research. By using isotopically labeled peptides as internal standards, AQUA allows for precise quantification of proteins and peptides. Due to its high sensitivity, specificity, and accuracy, AQUA technology has been widely adopted in fields such as biomedicine, drug discovery, and basic life sciences.
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• Principle of Absolute Quantitative Analysis
In proteomics research, quantitative analysis is essential for understanding the changes in protein expression levels under different biological conditions. Absolute Quantification (AQUA) is a precise and reliable method widely used in proteomics for accurate quantification. The AQUA technique relies on mass spectrometry (MS) and isotopically labeled standards to measure the amount of target proteins or peptides in an absolute manner.
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• Detection of N-Glycan Types in Glycosylated Proteins Based on LC-MS
Glycosylation is a significant post-translational modification process in living organisms, critically influencing the function, stability, and intercellular interactions of proteins. N-glycosylation, a specific form of glycosylation, typically occurs on the asparagine residues of proteins. The types and structures of N-glycans can vary among different organisms and cellular states, impacting biological functions and pathological conditions.
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• Steps of Glycosylation Site Analysis Using LC-MS/MS
Glycosylation is a crucial post-translational modification that profoundly impacts the structure and function of proteins. Identifying glycosylation sites is essential for understanding the roles of biomolecules within cells and their potential mechanisms in diseases. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) has emerged as an efficient analytical tool widely used in glycosylation site research.
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• Detection of N- and O-Glycosylation Sites Based on LC-MS/MS
Glycosylation is an essential modification of biomolecules, particularly proteins, significantly impacting their structure and function. N-glycosylation and O-glycosylation are the two primary forms of glycosylation, involving nitrogen and oxygen atoms of amino acid residues, respectively.
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• Quantitative and Qualitative Glycoprotein Analysis via Mass Spectrometry
Glycoproteins are important biomolecules widely found in living organisms, participating in various biological processes such as cell recognition, signal transduction, and immune response. The complexity of glycoprotein structure and function makes it a significant area of biological research. Recent advancements in mass spectrometry (MS) technology have provided a powerful tool for the quantitative and qualitative analysis of glycoproteins.
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• Steps of N-glycan Profiling Using Enzyme Digestion and MALDI-TOF-MS
N-glycomics research is a crucial field for exploring the role of N-glycosylation in the functions and interactions of molecules within biological systems. N-glycosylation plays a key role in various biological processes, and its abnormalities are often associated with diseases. The use of enzymatic digestion and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF-MS) allows for efficient analysis of the structure and composition of N-glycans.
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• N-glycan Profiling Based on MALDI-TOF-MS Detection
N-glycosylation is an important post-translational modification process in biological systems, significantly affecting protein function and stability. N-glycomics analysis is a vital tool for studying N-glycosylation, and the method based on MALDI-TOF-MS (Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry) has gained widespread attention for its high throughput and sensitivity.
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• Quantitative Glycomic Analysis Based on HILIC-UHPLC
Glycomics is the study of carbohydrate biomolecules, particularly their synthesis, metabolism, and functions in living organisms. With the growing understanding of the role of carbohydrates in cell function and diseases, quantitative analysis in glycomics has become increasingly important. HILIC-UHPLC (Hydrophilic Interaction Liquid Chromatography-Ultra High Performance Liquid Chromatography) is widely used in quantitative glycomics analysis due to its high separation efficiency and sensitivity.
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