Resources

Proteomics Databases



Metabolomics Databases

• • How to Determine Protein Full-Length Sequence

  Determining the protein full-length sequence is a foundational task in protein research, particularly crucial in studies involving novel protein functions, structural analysis, antibody development, and recombinant expression. But how can researchers obtain the complete amino acid sequence of a protein? This article provides a technical overview and practical strategies based on current mainstream approaches and recent advances. What Is a Protein Full-Length Sequence? A protein full-length sequence refers..

• • How Protein Full-Length Sequencing Accelerates Drug Development | MtoZ Biolabs

  What Is Protein Full-Length Sequencing? Protein full-length sequencing is defined as the determination of the complete amino acid sequence of a protein, spanning from the N-terminus to the C-terminus, using mass spectrometry (MS). This process also enables the identification of potential post-translational modifications (PTMs), isoforms, and contaminant proteins. Typically, it involves: 1. Multi-enzyme digestion followed by reconstruction of overlapping coverage regions 2. Integration of database search....

• • Full-Length Sequencing Strategies for Protein Isoform Characterization

  From subtle structural differences to functional assessment, how can protein full-length sequencing uncover the “hidden information” embedded within isoforms? In the development of macromolecular biopharmaceuticals—such as protein-based therapeutics, recombinant enzymes, and engineered antibodies—the presence of protein isoforms often represents a double-edged sword: On one hand, naturally occurring isoforms introduce structural diversity and serve as a foundation for functional regulation; On the other....

• • Ultimate Guide to Protein Full-Length Sequencing: Techniques, Applications, and Why It Matters

  The relationship between gene expression levels and protein function is not always directly proportional, making the direct measurement and analysis of proteins critically important. The advent of protein full-length sequencing has equipped scientists with a powerful means to decode the primary structure of proteins, significantly advancing fields such as biomedical research, precision medicine, and drug development. Over recent decades, protein sequencing technologies have evolved from classical Edman.....

• • Indrop Sequencing

  Indrop Sequencing is a high-throughput single-cell transcriptomic technology that enables the capture and profiling of gene expression at the individual cell level. Its core mechanism involves the use of microfluidic chips to encapsulate single cells along with barcoded hydrogel beads into individual oil droplets, allowing for the isolation of cellular RNA, reverse transcription, and library construction. As a representative method of droplet-based single-cell sequencing, Indrop Sequencing offers distinct..

• • Liposome Cryo-EM

  Liposome Cryo-EM refers to a technique for conducting in situ, high-resolution imaging of synthetically engineered liposomes using cryo-electron microscopy. This method combines the high precision of advanced electron microscopy with the biological relevance of liposome-based model systems, enabling structural and physicochemical characterization of liposomes under near-native conditions. These analyses include their ultrastructure, physical properties, and interactions with therapeutic molecules such as...

• • Anti Drug Antibody Analysis

  Anti Drug Antibody Analysis is a critical component in the development and clinical use of biopharmaceuticals. It is primarily conducted to assess whether patients undergoing biologic treatment elicit immune responses against the therapeutic agent itself. These immune responses typically involve the formation of antibodies targeting therapeutic protein drugs, such as monoclonal antibodies, fusion proteins, and enzyme replacement therapies. These antibodies are collectively referred to as Anti-Drug .........

• • De Novo Protein Sequencing Analysis Service

  De novo protein sequencing analysis service is an analytical method that determines the primary structure of proteins—namely, their amino acid sequence—directly through mass spectrometry, without the need for any pre-existing reference sequence. Unlike conventional protein identification approaches that depend on database matching, de novo protein sequencing is particularly well-suited for scenarios involving novel proteins, unknown post-translational modifications, antibody variable regions, or non-model..

• • Mass Cytometry Antibodies

  Mass cytometry antibodies are essential reagents employed in mass cytometry for labeling cell surface or intracellular targets. These antibodies are monoclonal in nature and are conjugated to specific metal isotopes. As a fundamental component of multiparameter cellular analysis, mass cytometry antibodies exhibit high specificity, minimal background interference, and broad compatibility. In conventional fluorescence-based flow cytometry, the number of measurable parameters is constrained by spectral........

• • Antibody Repertoire Analysis

  Antibody repertoire analysis aims to identify and isolate specific antibody molecules through in-depth characterization of diverse antibody repertoires. These antibodies play a crucial role in the immune system by specifically recognizing and neutralizing foreign antigens such as viruses and bacteria. Their function is indispensable in both the prevention and treatment of diseases. Antibody repertoire analysis has found broad application in drug development, diagnostic reagent discovery, and fundamental....