Resources

Proteomics Databases



Metabolomics Databases

• • SDS-PAGE Protein Identification

  SDS-PAGE protein identification is a widely utilized technique in biochemical and molecular biology research, primarily for the analysis and identification of complex protein mixtures. The acronym SDS-PAGE refers to Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis, a method that employs SDS, an anionic detergent, to denature proteins, thereby disrupting their native conformations and facilitating separation based on molecular weight. The principal function of SDS-PAGE protein identification i......

• • N-Terminal Sequence Analysis of Proteins and Peptides

  In the linear structure of proteins, the N-terminus (amino terminus) serves as both the starting point for polypeptide chain synthesis and a crucial determinant of protein function. From post-translational modifications and subcellular localization to enzymatic activity regulation and molecular interactions, even minor alterations in the N-terminal sequence can have profound effects on protein stability and functionality. As such, N-terminal sequence analysis of proteins and peptides is not only ........

• • SDS-PAGE Analysis of Purified Protein

  SDS-PAGE analysis of purified protein is a widely used method for examining and identifying polypeptide chains in protein samples. This technique enables the separation of proteins based on molecular weight by denaturing them and imparting a uniform negative charge, allowing for electrophoretic migration in a gel matrix. SDS-PAGE analysis of purified protein plays a crucial role in biochemistry and molecular biology, facilitating the separation and identification of proteins in research. It allows for......

• • De Novo Protein Sequencing: How to Improve Accuracy and Data Analysis Efficiency

  De novo protein sequencing is a mass spectrometry-based approach for determining protein amino acid sequences without relying on a reference genome or known protein database. Compared to database-dependent protein identification methods, this technique enables the characterization of novel proteins, peptide modification variants, and protein sequences from non-model organisms. However, due to the complexity of mass spectrometric data, the diversity of fragment ions, and the presence of background noise.....

• • Steps and Common Pitfalls in De Novo Protein Sequencing

  De novo protein sequencing is a mass spectrometry-based approach for determining protein amino acid sequences without relying on database references. This technique is widely employed in novel protein identification, antibody sequencing, and post-translational modification (PTM) analysis. While it has significant potential in studies of non-model organisms and protein engineering, challenges persist regarding data quality, algorithmic interpretation, and experimental execution. This review provides a ......

• • Overview of Protein De Novo Sequencing: Break Through Traditional Analysis Limitations

  In proteomics and biopharmaceutical research, determining the amino acid sequence (primary structure) of proteins is essential for elucidating their functions and biological significance. However, conventional protein sequencing methods primarily rely on database searches, making them inadequate for characterizing unknown proteins, novel variants, or unannotated species. As research advances, addressing this limitation has become increasingly critical. Protein de novo sequencing, a technique that dedu......

• • N-Terminal Sequencing: Unveiling the Basics of Edman Degradation and Its Key Applications

  N-terminal sequencing is a technique used to determine the amino acid sequence at the N-terminus of proteins, with extensive applications in proteomics, structural biology, and biopharmaceutical research. Among the available sequencing approaches, Edman degradation remains a classical and widely used method. This technique employs stepwise chemical degradation to sequentially cleave and identify N-terminal residues, enabling direct sequence determination. As a result, it provides valuable insights into.....

• • What Makes De Novo Protein Sequencing So Powerful? A Detailed Exploration

  De novo protein sequencing is a powerful technique for directly determining the amino acid sequences of proteins. It has become an indispensable tool in various fields, including the study of non-model organisms, antibody drug development, and the identification of protein mutations and post-translational modifications. The success of this approach depends on the seamless integration of multiple steps, including sample preparation, mass spectrometry analysis, data interpretation, and algorithm optimization.

• • MS-Based N-Terminal Sequencing: Sample Preparation and Data Analysis

  N-terminal sequencing is a critical approach in proteomics for determining the N-terminal amino acid sequence of proteins and peptides. It plays an essential role in investigating post-translational modifications, protein maturation, degradation pathways, and functional mechanisms. Compared to the traditional Edman degradation method, MS-based N-terminal sequencing has emerged as a powerful analytical tool due to its high sensitivity, high throughput, and compatibility with complex biological samples.......

• • Protein De Novo Sequencing: 10 Key Challenges and How to Overcome Them

  Protein de novo sequencing has shown distinct value in determining unknown amino acid sequences. However, the process still faces several significant challenges, spanning experimental design, data analysis, and biological complexity. These challenges require technological innovations and interdisciplinary approaches to overcome. This paper explores solutions for 5 key issues, integrating the latest advancements in technology.