Resources

Proteomics Databases



Metabolomics Databases

• • Amino Acid Analysis Using HPLC

  Amino acid analysis using HPLC is widely employed for the qualitative and quantitative assessment of amino acids in protein hydrolysates and raw materials. High-Performance Liquid Chromatography (HPLC) is a well-established separation technique in biochemistry and analytical chemistry, enabling the resolution of complex mixtures by exploiting the interactions between the stationary and mobile phases within a chromatographic column.   Amino acids, as fundamental building blocks of proteins, play crucia......

• • Proteomics Biomarker Discovery

  Proteomics biomarker discovery entails identifying proteins associated with specific diseases or biological states through the analysis of proteomes in cells, tissues, or body fluids. Beyond merely identifying proteins, this process aims to elucidate their functional roles and interactions in disease progression. The significance of proteomics biomarker discovery lies in its potential to enable early disease diagnosis and prognosis assessment. Given that many diseases-particularly cancer, cardiovascul......

• • Metabolomics Mass Spectrometry

  Metabolomics mass spectrometry is a technique used to study changes in metabolites within organisms. As an important branch of systems biology, metabolomics seeks to reveal metabolic pathways and physiological states by analyzing metabolites in cells, tissues, or whole organisms. These metabolites include amino acids, fatty acids, carbohydrates, nucleotides, and others, which directly participate in and regulate the biological processes of living organisms. Mass spectrometry, a highly sensitive analyt......

• • BioID&TurboID

  BioID&TurboID are biochemical techniques widely used to investigate intracellular protein interaction networks. These methods enable researchers to map complex protein interaction networks by labeling proteins that are in close proximity to or interact with specific target proteins. BioID, short for Biotin Identification, utilizes a mutant form of biotin ligase (BirA*) to attach biotin tags to nearby proteins. These tagged proteins can then be isolated and identified through affinity capture technique......

• • LC-MS Metabolomics

  LC-MS metabolomics is a powerful tool used for analyzing metabolites in complex biological samples. Metabolomics is the study of the composition, concentrations, dynamic fluctuations, and functional roles of small molecule metabolites in both physiological and pathological processes. By leveraging the separation capabilities of liquid chromatography and the identification power of mass spectrometry, detailed information about the metabolites in a sample can be obtained, including their types, concentr......

• • Tandem Mass Tag Labeling

  Tandem mass tag labeling is a widely applied chemical labeling technique in proteomics research. Its primary purpose is to allow researchers to simultaneously detect and compare the relative abundance of proteins from different samples within a single mass spectrometry analysis. This technique involves attaching isotopic tags to proteins or peptides, enabling the differentiation and quantification of identical molecules from different samples during mass spectrometry analysis. Tandem mass tag labeling......

• • Qualitative Test of Amino Acid

  The qualitative test of amino acid is an essential tool in biochemistry and molecular biology for identifying and analyzing the amino acid composition of proteins. Amino acids are the fundamental building blocks of proteins, and understanding their composition is crucial for elucidating the structure and function of proteins. In proteomics research, this test helps scientists determine the amino acid sequences of specific proteins or peptides. The technique is widely applicable across various fields, ......

• • Histone Modification Detection

  Comprehensive analysis of protein modifications represents a crucial method for investigating disease mechanisms and facilitating the development of novel therapeutics. This approach primarily employs mass spectrometry to perform extensive and precise assessments of protein modifications.

• • Detection of Post-Translational Modifications in Protein Drugs

  Post-Translational Modifications (PTMs) are chemical modifications that occur on proteins after their synthesis by mRNA translation, mediated by enzymatic activity on the protein's backbone or side chains. Types of PTMs: PTMs encompass various chemical modifications such as phosphorylation, acetylation, glycosylation, ubiquitination, and arginine methylation. These modifications alter the protein's chemical properties and charge distribution, consequently impacting protein structure and function. They can..

• • Western Blot Detection of Protein Phosphorylation Levels

  Western blotting is an immunoblotting technique extensively utilized for the detection of specific proteins within a sample. This method involves initially separating proteins via polyacrylamide gel electrophoresis (PAGE), followed by their transfer onto a membrane, and subsequent detection using specific antibodies. For assessing protein phosphorylation levels, specific antibodies targeting phosphorylated epitopes are employed. The following outlines the fundamental steps for using Western blotting to.....