Resources

Proteomics Databases



Metabolomics Databases

• • Protein Immunoblotting and Electrotransfer

  Protein immunoblotting, commonly known as Western Blotting, is a pivotal technique in molecular biology, biochemistry, and immunogenetics. This method is essential for detecting and analyzing specific proteins within a sample, providing valuable qualitative and semi-quantitative data.

• • Two Dimensional Gel Electrophoresis Service

  Two-dimensional gel electrophoresis (2-DE) is a crucial technique in proteomics, enabling the separation and analysis of complex protein mixtures from biological samples. This method separates proteins in two stages: isoelectric focusing (IEF), which separates proteins based on their isoelectric point (pI), and SDS-polyacrylamide gel electrophoresis (SDS-PAGE), which separates proteins by their molecular weight (Mr).

• • Protein Hydrolysis and Its Scientific Significance

  Protein hydrolysis is a significant topic in biochemistry, involving enzymatic reactions that break down proteins into peptides or amino acids. This process plays a crucial role in understanding protein structure and function, developing novel biomaterials, and advancing genetic engineering and pharmaceutical research. This article will introduce the basic principles of protein hydrolysis, the classification and applications of enzymes, and exemplify its importance in biomedical research.

• • 1D SDS-PAGE and IEF

  Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) is a fundamental technique extensively used in molecular biology and biochemistry for the separation and analysis of proteins. This method is invaluable in protein expression studies, providing detailed electrophoretic patterns of protein samples.

• • Advances in Proteomics

  Proteins are the direct executors of physiological functions, and studying their structure and function helps elucidate the mechanisms of changes under physiological or pathological conditions. Research on protein post-translational modifications, interactions, and conformations relies on direct protein studies. Therefore, a comprehensive understanding of life's complex activities necessitates studying proteins at a holistic, dynamic, and network level.

• • Gel Electrophoresis and Image Analysis

  Gel electrophoresis is a crucial technique for separating and analyzing biological macromolecules, widely used in proteomics and molecular biology research. This paper aims to introduce the basic principles, experimental procedures, and image analysis methods of gel electrophoresis, focusing on optimizing experimental conditions and improving data accuracy. This provides a comprehensive solution for researchers.

• • Detection of Differentially Expressed Proteins Using Statistical Methods

  Detecting differentially expressed proteins (DEPs) is a fundamental task in modern proteomics research, particularly in the investigation of disease mechanisms and the discovery of biomarkers. To extract biologically meaningful DEPs from complex proteomic data, scientists employ various statistical methods to ensure the reliability and accuracy of their results.   Basic Workflow of Detecting Differentially Expressed Proteins The detection of DEPs typically involves comparing protein expression levels ......

• • Sample Preparation and Proteomics Solutions

  Proteomics is the study of the overall structure and function of proteomes in living organisms. Sample preparation is a critical step in proteomics research, directly impacting the results of subsequent mass spectrometry (MS) analysis. This paper introduces the basic workflow and related solutions for proteomics sample preparation, aiming to provide effective methods and strategies for researchers.

• • De Novo Peptide Sequencing by Deep Learning

  Deep learning, characterized by its ability to automatically extract meaningful features from complex data via deep neural network architectures, has been extensively applied in domains such as image recognition, natural language processing, and bioinformatics. In recent years, it has also been increasingly employed in peptide sequence analysis. Applications range from de novo sequencing and peptide function prediction to antigen epitope identification and MHC binding affinity estimation, consistently .....

• • Error Analysis in Proteomics Data Assessment

  With the continued advancement of technologies, acquiring proteomics data has become more efficient and accessible. However, due to the inherent complexity of proteomics data and the limitations of current technologies, errors can arise during data evaluation. These errors may affect the accuracy and reliability of experimental outcomes.   Sources of Error in Proteomics Data Evaluation 1. Sample Preparation Sample preparation is a critical foundational step in proteomics research, as it directly......