Resources

Proteomics Databases



Metabolomics Databases

• • Antibody Peptide Mapping by LC-MS/MS: Workflow, Applications, and Quality Attributes

  A practical guide to antibody peptide mapping by LC-MS/MS, including digestion, peptide separation, sequence coverage, PTM identification, degradation monitoring, biosimilar comparability, and QC considerations.

• • What Is Label-Free Analysis? LFQ Principles, Workflow, Advantages, and Limitations

  A practical guide to label-free analysis and label-free quantification (LFQ), including MS1 intensity, spectral counting, LC-MS/MS workflow, normalization, missing values, batch effects, and comparison with labeling approaches.

• • Label Transfer in Protein Interaction Studies: Principle, Workflow, and Method Selection

  A practical guide to label transfer for protein interaction studies, including bait-prey labeling, activation, label transfer, MS detection, applications, controls, limitations, and method selection.

• • Top-Down Protein Sequencing Principle: Intact Protein MS, Fragmentation, and Proteoform Analysis

  A practical guide to top-down protein sequencing, including intact protein preparation, high-resolution MS, fragmentation, proteoform analysis, PTM preservation, applications, and limitations.

• • How Post-Translational Modifications Affect Protein Function?

  A practical guide to how post-translational modifications regulate protein activity, stability, localization, interactions, signaling, metabolism, disease mechanisms, and MS-based PTM analysis.

• • Glycan Profiling Methods vs Phosphoproteomics: Key Differences in Targets, Workflows, and Data

  A practical comparison of glycan profiling methods and phosphoproteomics, including analytical targets, sample preparation, LC-MS workflows, enrichment strategies, applications, limitations, and method selection.

• • Protein Analysis Mechanisms: How Extraction, Separation, Identification, and Quantification Work

  A practical guide to protein analysis mechanisms, including sample preparation, separation, LC-MS/MS identification, quantification, PTM analysis, structural methods, limitations, and method selection.

• • 4D Label-Free Quantitative Proteomics Workflow: From Sample Prep to Data Interpretation

  A practical guide to the 4D label-free quantitative proteomics workflow, including sample preparation, ion mobility, PASEF acquisition, data analysis, QC, and method limitations.

• • Protein Interaction Network Analysis for Disease Research and Target Discovery

  A practical guide to protein interaction network analysis, including disease mechanism research, drug target discovery, omics integration, method selection, and evidence-quality limits.

• • SPR vs. BLI vs. ELISA: Comparing the Advantages and Limitations of Three Antibody Assay Techniques

  The investigation of antibody-antigen interactions represents a central component of life sciences, drug development, and immunodiagnostics. The selection of an appropriate analytical technique is critical for ensuring the accuracy of experimental results and the reliability of data interpretation. Surface Plasmon Resonance (SPR), Bio-Layer Interferometry (BLI), and Enzyme-Linked Immunosorbent Assay (ELISA) are currently among the most widely employed antibody detection methods. Each technique has dis......