Resources

Proteomics Databases



Metabolomics Databases

• • SWATH Quantitative Proteomics of Plant Root Systems

  Plant root system is not only an organ for absorbing water and nutrients, but also a highly complex and dynamic biological system. The root system plays a crucial role in plant life processes, from germination, growth, response to environmental stress, to interaction with microorganisms in the soil, with each step involving specific proteins. SWATH quantitative proteomics enables us to accurately quantify and analyze these proteins.

• • Quantitative Proteomics Analysis Using iTRAQ/TMT

  Quantitative proteomics is an essential technology in biomedical research, providing detailed information on protein expression levels within cells. iTRAQ (isobaric tags for relative and absolute quantitation) and TMT (Tandem Mass Tag) are two widely used tagging techniques that enable relative and absolute quantification of multiple samples.

• • Procedure of Label Transfer in Protein Interaction Analysis

  Protein interactions are of great significance in cell biology and biomedical research. Understanding these interactions can reveal cellular signaling pathways and provide new targets for drug development. Label transfer technology is one effective method for analyzing protein interactions, enabling the tracking of proteins' dynamic changes within cells by introducing specific labels or probes.

• • Quantitative Analysis of Disulfide Bonds in Proteins Based on LC-MS/MS

  Proteins play a vital role in the normal functioning of biological systems, and the formation of disulfide bonds is a key aspect of protein structural stability. Disulfide bonds, typically found in extracellular environments, link two cysteine residues within a protein. Their presence is crucial for protein folding, stability, and regulatory activities. However, under conditions of protein dysfunction, disease, or environmental changes, disulfide bond formation and dissociation may be regulated.

• • Application of Multiple Reaction Monitoring

  Multiple Reaction Monitoring (MRM) is a highly sensitive mass spectrometry technique widely used in proteomics, metabolomics, and clinical diagnostics. Its core principle lies in the selection of specific parent and product ion pairs for quantitative analysis of target molecules, allowing MRM to accurately detect low-abundance biomarkers in complex samples.

• • Mechanism of Multiple Reaction Monitoring

  Multiple Reaction Monitoring (MRM) is a powerful mass spectrometry technique widely employed in fields such as proteomics, metabolomics, and drug development. Its primary advantage lies in its ability to quantitatively analyze specific target molecules in complex samples, thereby providing high sensitivity and selectivity in data acquisition.

• • Workflow of Multiple Reaction Monitoring

  Multiple Reaction Monitoring (MRM) is an important method in mass spectrometry, widely applied in biomedical research and drug development. MRM enables high sensitivity and specificity analysis in complex biological samples, making it a core tool in quantitative proteomics and metabolomics.

• • Advantages and Disadvantages of Multiple Reaction Monitoring

  Multiple Reaction Monitoring (MRM) is a widely used technique in mass spectrometry, primarily for quantitative analysis in targeted proteomics. By selecting specific precursor and product ion pairs, MRM enables sensitive monitoring of target analytes. Although MRM shows significant advantages in various fields, it also has limitations.

• • Mechanism of Parallel Reaction Monitoring

  The parallel reaction monitoring mechanism is an important concept in modern biological research, playing a crucial role in fields such as drug development, metabolic engineering, and biosensors. By monitoring the simultaneous occurrence of multiple reactions, researchers can gain deep insights into the behavior of biological systems and provide important data support for optimizing experimental conditions and improving yields.

• • Principle of Multiple Reaction Monitoring

  Multiple Reaction Monitoring (MRM) is a sensitive analytical method based on mass spectrometry, widely used for the detection and quantification of biomarkers in fields such as proteomics, drug metabolism, and environmental monitoring.