Resources

Proteomics Databases



Metabolomics Databases

• • What Are the Methods for Label-Free Quantitative Proteomics Analysis

  Label-Free Quantitative Proteomics (LFQ) is a technique used for determining relative protein quantities without the necessity for labeling or isotope tags. This method quantifies proteins by directly comparing the mass spectrometry signals across different samples. The following are several prevalent LFQ methods: Peak Area or Intensity-Based Methods: This approach quantifies protein abundance by comparing the mass spectrometry peak areas or intensities of identical peptide fragments across samples.

• • Quantitative Phosphoproteomics Requires the Amount of Peptides

  In quantitative phosphoproteomics analysis, peptide abundance is a critical factor, as it directly impacts the sensitivity, reproducibility, and quantitative accuracy of the experiments. The following are key considerations and suggestions: Sample Preparation: 1. Protein Extraction: Extract total proteins from cell or tissue samples, typically requiring several milligrams. 2. Protein Digestion: Using proteolytic enzymes like trypsin, digest the proteins into peptides. Generally, 1-5 mg of protein can yield

• • Proteomics Clustering Analysis

  Proteomics clustering analysis is a widely utilized statistical method for examining protein expression patterns and functions. By categorizing proteins with similar attributes, researchers can gain deeper insights into their roles and interactions. Steps in Proteomics Clustering Analysis: 1. Data Collection: Researchers initially gather protein expression data, often through techniques such as mass spectrometry or two-dimensional electrophoresis. 2. Data Preprocessing: This step involves preprocessing the

• • Is Labeling Required for 4D-DIA Quantitative Proteomics

  4D-DIA (Four-Dimensional Data-Independent Acquisition) is an innovative proteomics technique designed for comprehensive quantitative protein analysis. This method synergizes four dimensions of information: time, ion mobility, mass, and intensity, thereby enhancing the accuracy and efficiency of protein identification and quantification. In the context of 4D-DIA proteomics, conventional chemical labeling methods such as iTRAQ or TMT are generally not necessary for quantification. The critical steps and......

• • Liquid Chromatography-Circular Dichroism

  LC-CD (Liquid Chromatography-Circular Dichroism) is a powerful analytical method that integrates liquid chromatography (LC) with circular dichroism (CD) to analyze and identify biomolecules, particularly chiral compounds. This technique combines the separation power of LC with the structural information provided by CD, making it highly applicable in fields such as drug discovery, biochemistry, and environmental sciences.

• • Acetylation 4D-Label-Free Quantitative Proteomics

  Acetylation 4D-label-free quantitative proteomics is an advanced approach for investigating protein acetylation modifications and quantifying protein expression levels. By integrating the advantages of 4D proteomics with label-free quantification, this technique offers highly sensitive and high-resolution data on acetylated proteins. Herein, we provide an in-depth description of this methodology:

• • Magnetic Circular Dichroism Introduction

  Magnetic Circular Dichroism (MCD) is a spectroscopic technique used to investigate the magnetic properties of materials. It is based on the interaction between circularly polarized light and the magnetic characteristics of a material, enabling the analysis of its electronic structure under varying magnetic fields and optical conditions. Fundamental Principle: Magnetic Circular Dichroism (MCD) is based on the interaction between the optical activity of polarized light and the magnetic properties of materials

• • Quantitative Analysis of Metals

  Quantitative analysis of metals is a crucial aspect of chemical analysis, focusing on determining the elemental composition of metal samples through various chemical and physical methodologies. This type of analysis is vital across numerous industrial sectors, including metallurgy, environmental protection, and pharmaceuticals. Analysis Methods: 1. Titration: This common technique involves the addition of a reagent with known concentration to a sample until the reaction is complete, offering high accuracy..

• • Epitope Analysis of Antibody Binding

  Epitope analysis of antibody binding are specific regions within antigen molecules responsible for recognizing and binding specific antibodies. Analyzing these epitopes provides insight into the intricate interactions within antibody-antigen complexes and aids in understanding the specificity and cross-reactivity of antibodies or cell receptors. Technical Approaches: 1. Linear Epitope Analysis: Linear epitopes are characterized by continuous amino acid residues that serve as the binding site for antibodies

• • Methods for Determining Protein Molecular Weight

  Determining protein molecular weight is a fundamental task in the fields of biochemistry and molecular biology. A variety of techniques are available for this purpose, each characterized by its specific range of applications and level of accuracy. Below are several commonly employed methods for determining protein molecular weight: 1. SDS-Polyacrylamide Gel Electrophoresis (SDS-PAGE) SDS-PAGE is a widely used protein separation technique that enables the estimation of protein molecular weight.