Resources

Proteomics Databases



Metabolomics Databases

• • Peptide Analysis by Mass Spectrometry

  Peptide analysis by mass spectrometry is pivotal in proteomics research, serving as a technique to determine compounds' mass by measuring the mass-to-charge ratio of ions. This method combines mass spectrometry with protein digestion, where proteins are enzymatically broken down into peptides, which are then analyzed to obtain precise mass data. Its applications span basic biological research, clinical diagnostics, drug development, and sectors such as agriculture and environmental science.   In basic......

• • Peptide Identification by Mass Spectrometry

  Peptide Identification by Mass Spectrometry is a cornerstone technique in proteomics research, enabling the identification and quantification of protein peptides. Its primary applications include unraveling protein sequences, analyzing protein modifications, and studying protein-protein interactions. In proteomics, understanding the structure and function of proteins is paramount, and mass spectrometry provides a precise, rapid, and high-throughput approach to achieve this. Mass spectrometers ionize p......

• • 4D Proteomics for Advanced Protein Analysis

  4D proteomics analysis enhances traditional 3D proteomics-which considers retention time, mass-to-charge ratio, and ion intensity-by adding ion mobility as a fourth dimension. This advanced technique not only focuses on three-dimensional structural studies but also incorporates a temporal dimension, thus offering more comprehensive and dynamic insights into biological systems. Integrating mass spectrometry, time-resolved techniques, and sophisticated data analysis, 4D proteomics reveals the dynamic ch......

• • Palmitoylation Mass Spectrometry

  Palmitoylation mass spectrometry is a sophisticated analytical technique employed to detect and characterize lipid modifications in proteins, particularly palmitoylation. This modification involves the covalent attachment of palmitoyl groups to specific amino acid side chains via ester or thioester bonds, which is critical for modulating protein function, localization, and stability. Given its dynamic and reversible nature, palmitoylation plays a pivotal role in cellular signaling pathways, protein-pr......

• • 2-DE Proteomics

  2-DE proteomics is a comprehensive analytical technique that investigates the expression, structure, function, interactions, and dynamic changes of proteins within cells, tissues, or organisms. This technique integrates isoelectric focusing (IEF) and SDS-PAGE, enabling the high-resolution separation of proteins based on their isoelectric points and molecular weights. In the first dimension, IEF separates proteins by their isoelectric points (pI) within a pH gradient, concentrating them at their respec......

• • 2D-DIGE Analysis

  2D-DIGE Analysis (Two-Dimensional Difference Gel Electrophoresis) integrates traditional two-dimensional electrophoresis with fluorescent labeling technology, enabling the resolution and quantification of multiple protein samples on a single gel. Its core advantages are high resolution and sensitivity, essential for comparing protein expression profiles. Unlike conventional two-dimensional electrophoresis (2-DE), 2D-DIGE innovatively uses fluorescent dyes such as Cy2, Cy3, and Cy5 to label different p......

• • SILAC-MS

  SILAC-MS is a widely employed technique for investigating the dynamic changes and functions of intracellular proteins. This method involves the incorporation of stable isotopes of light and heavy amino acids into cell cultures, facilitating quantitative protein analysis. The fundamental principle of SILAC-MS is the replacement of natural amino acids with isotopically labeled ones, introducing distinguishable mass differences during protein synthesis. These differences are highly discernible in mass sp......

• • Single-Cell Phosphoproteomics

  Single-cell phosphoproteomics integrates single-cell analysis with phosphoproteomics, enabling high-throughput examination of protein phosphorylation modifications at the single-cell level. This approach offers new insights into intracellular signal transduction and the regulation of biological processes. Protein phosphorylation is a prevalent post-translational modification that plays a crucial role in regulating diverse cellular functions such as signal transduction, metabolic control, cell cycle pr......

• • Saliva Proteomics for Disease Diagnosis

  Saliva proteomics investigates the protein composition and functions present in saliva, primarily through the analysis of saliva protein expression profiles using advanced proteomic technologies. As a biological fluid that is easy to collect and non-invasive, saliva serves as an ideal sample source for disease diagnosis and monitoring. It contains a complex mixture of proteins, enzymes, antibodies, and microorganisms that can reflect the physiological and pathological states of the human body. Researc......

• • Antibody Sequencing Methods and Applications

  Antibody sequencing refers to the process of determining the genetic or amino acid sequence of an antibody (immunoglobulin). Antibodies are key proteins of the immune system that recognize and neutralize foreign substances such as bacteria and viruses. A comprehensive understanding of antibody sequences is crucial for developing novel vaccines, designing therapeutic antibody drugs, and gaining deeper insights into immune system mechanisms. The core of antibody sequencing lies in accurately analyzing a......