Resources

Proteomics Databases



Metabolomics Databases

• • Advantages and Disadvantages of Label Transfer in Protein Interaction

  In protein interaction studies, the label transfer method, combined with crosslinking techniques, provides an effective means of marking proteins that interact with target proteins. This technique not only facilitates the discovery of new interactions but also validates proteins identified by other methods and analyzes protein complexes.

• • Principle of Label Transfer in Protein Interaction Analysis

  The core of the label transfer method involves transferring a label from one protein (typically referred to as the "bait") to another interacting protein (referred to as the "prey"). This method leverages the photosensitive properties of crosslinkers, which form covalent bonds between two interacting proteins when exposed to light at a specific wavelength.

• • Mechanism of SILAC and Dimethyl Labeling in Quantitative Proteomics

  The advancement of quantitative proteomics has significantly enhanced our understanding of protein expression, modification, and interaction within biological systems. Among various quantitative proteomic techniques, SILAC (Stable Isotope Labeling by Amino acids in Cell culture) and dimethyl labeling have become widely adopted due to their high sensitivity and quantitative accuracy.

• • Application of SILAC and Dimethyl Labeling in Quantitative Proteomics

  Stable Isotope Labeling by Amino acids in Cell culture (SILAC) and Dimethyl Labeling are two pivotal techniques in quantitative proteomics. As proteomics research progresses, scientists increasingly depend on these techniques for the precise quantification of protein expression changes.

• • Workflow of SILAC and Dimethyl Labeling in Quantitative Proteomics

  Quantitative proteomics often relies on methods such as SILAC (Stable Isotope Labeling by Amino acids in Cell culture) and dimethyl labeling for comparing the relative abundance of proteins across different samples. Each method has distinct advantages, making them suitable for various experimental conditions.

• • Advantages and Disadvantages of SILAC and Dimethyl Labeling in Quantitative Proteomics

  Quantitative proteomics is a critical technology for studying changes in protein expression under various biological conditions. Among the numerous quantitative proteomics techniques, SILAC (Stable Isotope Labeling by Amino acids in Cell culture) and dimethyl labeling are two widely adopted methods.

• • Principle of SILAC and Dimethyl Labeling in Quantitative Proteomics

  Proteomics is the study of the composition, structure, function, and dynamic changes of proteins within cells or tissues. In quantitative proteomics, researchers employ various labeling techniques to measure the relative or absolute abundance of proteins, with stable isotope labeling being highly regarded for its precision and reliability.

• • How to Calculate the Isoelectric Point of Amino Acids?

  The isoelectric point (pI) of an amino acid refers to the pH value at which the net charge of the amino acid molecule is zero. The basic method for calculating the pI of an amino acid involves considering the pKa values (negative logarithm of acid dissociation constants) of the functional groups in the amino acid molecule, and using these values to find the pH value at which the net charge of the amino acid molecule is zero.

• • Chiral Circular Dichroism of Amino Acids

  Due to the presence of a chiral center on the α-carbon, amino acids exhibit optical activity. The two stereoisomers of chiral amino acids are referred to as L and D forms. The majority of naturally occurring amino acids in proteins are of the L form. As these amino acids are optically active, they interact with plane-polarized light, resulting in the rotation of the light.

• • Peptide Drug Elemental Analysis

  Peptide drugs are biologically active molecules composed of multiple amino acids linked by peptide bonds. Typically, they consist of 10-100 amino acids and have a molecular weight below 10,000. Peptide drugs are mostly derived from endogenous or natural peptides, therefore they have little or no side effects on the human body.