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Proteomics Databases



Metabolomics Databases

• • Methods and Applications of Protein Circular Dichroism (CD) Spectroscopy

  Circular Dichroism (CD) spectroscopy is a chiroptical technique that detects the differential absorption of left- and right-circularly polarized light by chiral molecules. It has been widely employed to investigate protein secondary structure, conformational dynamics, thermal stability, and folding pathways. Owing to its non-destructive nature, high sensitivity, and ease of use, CD spectroscopy has become an essential tool for protein structural characterization. In the fields of life sciences and ......

• • How to Interpret High-Dimensional Data in Single Cell Proteomics

  Single cell proteomics (SCP) is rapidly emerging as a powerful tool for dissecting cellular heterogeneity and the dynamic nature of biological processes. By profiling protein expression at the resolution of individual cells, researchers can gain critical insights into the molecular underpinnings of tissue homeostasis, disease progression, immune responses, and more. However, SCP data are inherently high-dimensional, typically involving the quantification of hundreds to thousands of proteins per cell........

• • Integrating Single Cell Proteomics with scRNA-seq: Methods and Tools

  The rapid advancement of single-cell sequencing technologies has enabled researchers to dissect cellular heterogeneity at single-cell resolution. Among various approaches, single-cell RNA sequencing (scRNA-seq) primarily captures the transcriptomic landscape by profiling mRNA expression, whereas single cell proteomics (scProteomics) offers insights into protein-level expression, which more directly reflects functional cellular output. These two modalities represent distinct yet complementary dimension......

• • Using CD Spectroscopy to Evaluate Protein Thermal Stability

  Protein thermal stability is a key indicator of structural integrity and functional retention, and it is widely applied in basic research, protein engineering, and biopharmaceutical development. Circular Dichroism (CD) spectroscopy is a sensitive, rapid, and low–sample-volume technique commonly used to monitor conformational changes in protein secondary structure. It is particularly suitable for evaluating thermal denaturation behavior and thermal stability parameters such as the melting temperature .......

• • How to Analyze CD Spectra Using Deconvolution Algorithms

  Circular Dichroism (CD) spectroscopy is an important technique for investigating the secondary structures of proteins. However, a CD spectrum essentially represents the superposition of multiple structural components (such as α-helices, β-sheets, and random coils), and thus cannot directly reveal the proportion of each constituent. In such cases, the deconvolution algorithm serves as a crucial analytical approach. This study systematically presents the principles, workflow, and considerations for appl......

• • Circular Dichroism Analysis of Antibody Drugs

  In antibody drug development, structural characterization is a critical step to ensure both safety and therapeutic efficacy. Circular dichroism (CD) is an optical technique that relies on the differential absorption of left- and right-circularly polarized light by chiral molecules. Owing to its operational simplicity, high sensitivity, and minimal sample requirement, Circular Dichroism analysis has become a widely employed method for investigating protein secondary structures.   Introduction to Circul......

• • DIA-MS Analysis Solutions for Diverse Sample Types: Plasma, Tissue, and More

  In proteomics research, achieving high reproducibility across biologically diverse and complex sample types remains a persistent challenge. A central focus of mass spectrometry development is how to enable high-throughput, low-bias quantitative analysis for a wide range of biological specimens, including plasma, tissue, cells, and body fluids. Data-Independent Acquisition Mass Spectrometry (DIA-MS), with its high proteome coverage, superior reproducibility, and minimal missing values, is emerging as a .....

• • Far UV Circular Dichroism (CD) Spectroscopy Analysis

  In structural biology, the precise determination of protein secondary structures is fundamental to elucidating their functions. Far-UV Circular Dichroism (CD) spectroscopy, a highly sensitive and non-destructive technique, is widely employed for investigating protein conformations, assessing structural stability, and analyzing drug–protein interactions across diverse research fields. What is Far-UV Circular Dichroism (CD) Spectroscopy? Circular Dichroism (CD) refers to the differential absorption of l......

• • CD Spectroscopy vs FTIR: Which Technique is Better for Analyzing Protein Secondary Structure?

  The structural organization of a protein dictates its biological function, with secondary structure representing a pivotal level in this hierarchy. Alterations in α-helices, β-sheets, and random coils are frequently associated with functional transitions of proteins, including partial or complete inactivation. Consequently, accurate characterization of protein secondary structure is essential not only for fundamental research but also for applications such as drug development, evaluation of biopharmac......

• • How to Use CD Spectroscopy for Protein Secondary Structure Analysis: A Complete Guide

  Circular Dichroism (CD) spectroscopy is a technique that measures the differential absorption of circularly polarized light by chiral molecules, and it is widely employed for rapid qualitative and quantitative assessment of protein secondary structures. In contrast to high-resolution approaches such as X-ray crystallography or nuclear magnetic resonance (NMR), CD spectroscopy offers distinct advantages, including rapid data acquisition, no requirement for crystallization, and minimal sample consumptio......