Resources

Proteomics Databases



Metabolomics Databases

• • Therapeutic Target Identification

  Therapeutic target identification is an essential component of contemporary drug development and precision medicine research. It focuses on systematically identifying molecular targets that are intimately associated with disease pathogenesis and progression. These targets typically include specific proteins, genes, or other biomolecules that are pivotal in pathological processes. The primary aim of therapeutic target identification is to pinpoint these critical molecules and validate their potential t......

• • Protein Sequence Conservation Analysis

  Protein sequence conservation analysis is a bioinformatics approach used to investigate the evolutionary preservation of specific sequences within proteins. This analysis is instrumental in identifying crucial structural and functional characteristics of proteins, as conserved sequences are typically linked to essential biological functions and structural domains. The extent of sequence conservation often reflects the functional significance of a protein across different species and the evolutionary p......

• • Pull-Down Assay for Protein-Protein Interaction

  A pull-down assay for protein-protein interaction is a powerful tool used to investigate interactions between proteins. This technique is mainly employed to study direct interactions between proteins, as well as interactions between proteins and other biomolecules such as DNA, RNA, and lipids. Studying these interactions provides insights into molecular mechanisms underlying critical cellular processes like signal transduction, metabolic regulation, and gene expression control. The pull-down assay for......

• • Protein Quantitation Using Mass Spectrometry

  Protein quantitation using mass spectrometry represents a pivotal tool in modern proteomics. By leveraging mass spectrometers, this technique enables precise detection and quantification of proteins in biological samples, thus providing scientists with crucial insights into protein expression, interactions, and functional states. As the functional molecules within organisms, proteins undergo dynamic changes in quantity and function, playing vital roles in biological processes. The major advantage of t......

• • Protein Secondary Structure Analysis in Drug Development

  Protein secondary structure analysis techniques are pivotal for revealing the local structural characteristics within protein molecules. These secondary structures-comprising α-helices, β-sheets, and random coils-are stabilized by hydrogen bonds and form the foundation for the protein's tertiary structure and function. They directly influence the protein's activity, stability, and interactions within biological systems. By employing protein secondary structure analysis, researchers can unravel the fun......

• • Protein Identification by MALDI-TOF Mass Spectrometry

  Protein identification by MALDI-TOF mass spectrometry is an efficient technology for protein identification, widely utilized in proteomics research. This technique combines MALDI and TOF mass spectrometry to achieve soft ionization of protein molecules, facilitated by matrix molecules that absorb laser energy and transfer it to the proteins, generating charged ions. These ions, accelerated through an electric field into a flight tube, have different flight times based on their mass-to-charge ratios (m......

• • Protein Glycosylation Analysis

  Protein glycosylation analysis techniques are essential for investigating the glycosylation modifications on proteins. By examining the glycosylation states and glycan structures on protein molecules, researchers can gain insights into how this vital post-translational modification influences biological functions. Glycosylation is one of the most prevalent and intricate post-translational modifications and plays a role in numerous biological processes, such as cell-cell communication, protein folding,......

• • Protein Gel Analysis

  Protein gel analysis is a classical and widely utilized technique in protein research, instrumental in separating and analyzing protein components in biological samples. This method allows researchers to visually assess the molecular weight distribution, purity, and expression levels of proteins, providing a foundation for further functional studies and quantitative analyses. The core principle of protein gel analysis involves the use of electrophoresis to separate proteins based on molecular weight o......

• • Protein Identification by LC-MS

  Protein identification by LC-MS stands as a cornerstone technology in modern proteomics, combining the effective separation capability of liquid chromatography (LC) with the sensitive detection of mass spectrometry (MS). This technique enables high-throughput protein identification and characterization in complex samples by breaking down proteins into peptides, which are then individually separated and detected for precise molecular-level analysis. Initially, samples undergo protein extraction and enz......

• • Protein Expression Profiling

  Protein expression profiling is a scientific approach used to analyze the composition and abundance changes of proteins within cells, tissues, or whole organisms. This research area is crucial for understanding biological functions, as proteins are the direct functional molecules responsible for carrying out life processes. By implementing protein expression profiling, scientists can identify protein groups that exhibit differential expression under varying physiological or pathological conditions, wh......