Resources
Proteomics Databases
Metabolomics Databases
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Yeast 2 hybrid analysis is a molecular biology technique used to study protein-protein interactions. First developed by Fields and Song in 1989, the method exploits the transcription activation mechanism in yeast cells to detect whether two proteins are capable of interacting with each other. The basic principle of yeast 2 hybrid analysis involves fusing the proteins of interest to the activation domain and DNA binding domain of a transcription factor. When expressed in yeast cells, if the two protein......
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• FTIR Analysis of Protein Structure
FTIR analysis of protein structure is an analytical technique used to study the secondary structure and conformational changes of proteins. By performing FTIR analysis, vibrational information about various chemical bonds in protein molecules can be obtained, allowing the structural features of the protein to be inferred. This method is particularly suitable for investigating conformational changes in proteins under different environmental conditions, such as variations in temperature, pH, or solvent ......
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GC-MS amino acid analysis is a method employing gas chromatography-mass spectrometry (GC-MS) technology for the qualitative and quantitative analysis of amino acids. This technique is particularly suitable for analyzing amino acids in complex biological samples. Amino acids are the fundamental building blocks of proteins and also serve as key molecules in many metabolic pathways within organisms. Accurate analysis of the types and concentrations of amino acids is of significant importance in life scie......
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Signal peptide analysis is mainly applied to investigate the role of signal peptides in protein synthesis and secretion. A signal peptide is a short amino acid sequence, typically located at the N-terminus of newly synthesized proteins, that directs the cellular localization and secretion pathways of these proteins. The primary function of the signal peptide is to guide proteins across the cell membrane into organelles such as the endoplasmic reticulum, mitochondria, or chloroplasts, or to facilitate ......
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• Drug Target Identification Methods
Drug target identification methods play an essential role in modern medical research. These techniques not only help uncover the molecular mechanisms of diseases but also provide the core foundation for the development of new drugs. Drug target identification methods primarily include various techniques, such as computational biology, proteomics, chemical biology, and systems biology. Computational biology predicts potential drug targets by analyzing large-scale data and applying algorithmic models. I......
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Shotgun proteomic analysis is a highly efficient and comprehensive method for proteomic research. This approach employs mass spectrometry to examine complex protein mixtures, with a fundamental strategy involving enzymatic digestion of proteins, followed by mass spectrometric analysis and identification of the resulting peptides. Shotgun proteomic analysis is particularly effective for investigating intricate biological samples, including tissue extracts, cell lysates, and plasma. It enables the chara......
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• Drug Target Identification and Validation
Drug target identification and validation are integral steps in the drug development process. A drug target refers to a biological molecule or a group of molecules that play a crucial role in the onset and progression of a disease. Drugs achieve therapeutic effects by interacting with these targets. Validation confirms the function and biological significance of the identified targets, ensuring they can produce the anticipated therapeutic effects in both in vitro and in vivo experiments. The importanc......
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• Proteomic Analysis Mass Spectrometry
Proteomic analysis mass spectrometry is a powerful technique used to study the types, structures, functions, and interactions of proteins within cells, tissues, or organisms. As a central tool in proteomics research, mass spectrometry is widely applied across multiple fields, including biomedicine, drug development, disease diagnosis, and agricultural sciences. By employing high-precision mass measurements and advanced data processing techniques, proteomic analysis mass spectrometry can identify and q......
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PTM identification is a critical technique in modern proteomics. Post-translational modifications (PTMs) refer to the chemical modifications that proteins undergo after translation through enzymatic reactions, which can profoundly alter their properties and functions. PTMs are a key source of protein functional diversity in living organisms, regulating a range of biological processes, such as signal transduction, the cell cycle, metabolic control, and gene expression, by modifying protein structure, s......
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Proteomics quantification is a technique designed to measure the quantity of proteins in biological samples, with the primary aim of elucidating changes in protein roles across various biological processes. By assessing protein abundance quantitatively, either relatively or absolutely, this technique facilitates insights into biological function under different experimental or natural conditions. Proteomics quantification has a broad range of applications, including the identification of disease bioma......
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