Resources

Proteomics Databases



Metabolomics Databases

• • Challenges in Single-Cell Mass Spectrometry Analysis

  Mass spectrometry is a powerful analytical technique extensively utilized across diverse research domains, including biomedical research, pharmaceutical development, and environmental studies. As technological advancements have been made, mass spectrometry has progressed from assessing bulk tissues and cell populations to examining individual cells, termed single-cell mass spectrometry. This advancement, however, introduces new complexities rather than simplifying the analytical processes.

• • What Concentration of Exosomes Is Generally Used for Protein Detection

  Exosome detection is a crucial step in biological research, particularly in the study of exosomal proteins. However, determining the optimal concentration of exosomes for protein detection remains a critical challenge. This paper aims to address this issue and provide guidance for researchers in selecting the appropriate concentration for experimental applications. Introduction to Exosomes: Exosomes are nano-sized vesicles, typically measuring between 30 to 150 nanometers in diameter. They are secreted.....

• • Do Proteins Need to Go Through Liquid Chromatography for Mass Spectrometry

  Mass spectrometry (MS) is a robust analytical technique that provides detailed insights into protein composition and structure. Integrating liquid chromatography (LC) with MS has become essential for studying protein attributes. Liquid Chromatography Overview: Liquid chromatography is a laboratory technique designed to separate mixture components. It involves dissolving the sample in a liquid phase and passing it through a stationary phase, where interactions influence component migration rates, enabling...

• • Mass Spectrometry Negative Ion Mode Detection of Polypeptides

  Mass spectrometry (MS) is an essential analytical tool that enables a comprehensive understanding of protein composition and structure. In proteomic research, the combination of liquid chromatography (LC) and mass spectrometry has become a widely adopted strategy, significantly enhancing the accuracy and depth of protein characterization.

• • Preprocessing for Tandem Mass Spectrometry Protein Analysis

  Tandem mass spectrometry (MS/MS) is a powerful analytical technique widely used for protein identification and quantification. However, successful MS/MS analysis relies heavily on meticulous sample preparation, as the quality of preparation directly influences the reliability and accuracy of the results. 1. Protein Extraction: The first step involves extracting and purifying proteins from biological samples. Various extraction methods are available, including mechanical disruption, enzymatic digestion, and.

• • Protein Mass Spectrometry Sequencing Depth

  The depth of coverage in protein mass spectrometry is a fundamental parameter in proteomics research, as it directly influences the characterization of the protein composition within a sample. It is widely regarded as a key indicator of the quality and comprehensiveness of mass spectrometry-based proteome analyses. Definition and Importance of Sequencing Depth: The depth of coverage in protein mass spectrometry refers to the number of proteins or peptides detected in a single proteomics experiment. This....

• • Cellular IP Product Mass Spectrometry Detection

  Immunoprecipitation (IP) combined with mass spectrometry (MS) is a widely used approach for investigating protein-protein interactions and quantifying protein expression levels. The integration of these techniques enables molecular-level analysis of biological processes, providing detailed insights into protein function and cellular mechanisms.

• • Protein Mass Spectrometry Analysis of Antigen Types

  The immune response is the physiological reaction of an organism to foreign antigens, initiated by antigen-antibody interactions. Antigens, typically proteins or polysaccharides, provoke an immune response. Accurate identification and classification of antigens are vital for understanding and manipulating immune responses. Recently, protein mass spectrometry analysis has been employed to identify and classify antigens.

• • Proteomics Can Analyze Specific Functional Proteins

  Proteomics is a scientific discipline dedicated to investigating protein expression, localization, interactions, and functions within cellular systems. It provides critical insights into the complexity and dynamic nature of biological processes. This paper explores the application of proteomics in the analysis of proteins with defined functional roles. Introduction to Proteomics: Proteins act as crucial functional components across all biological levels, from molecular systems to entire ecosystems. Unlike..

• • Exosome Peptide Detection

  Exosomes are extracellular vesicles secreted by cells throughout their lifecycle. They typically measure between 30 and 150 nanometers in diameter and are enclosed by a phospholipid bilayer, allowing them to exist freely in various body fluids. The cargo of exosomes includes proteins, lipids, RNA, and DNA, rendering them ideal for indicating cellular status and function.