Resources

Proteomics Databases



Metabolomics Databases

• • Post-Translational Modifications: Key to Regulating Bioactivity

  Post-translational modification (PTM) of proteins refers to the chemical modifications that occur on proteins after they are translated from mRNA. These modifications involve chemical changes to the protein structure through a series of biochemical reactions, regulating its function, stability, location, and interactions. PTMs can occur on amino acid residues or on the overall protein structure.

• • Identification and Methods of O-Glycosylation

  O-glycosylation is an important form of glycosylation, where sugars are linked to proteins or peptides through O-glycosidic bonds involving oxygen (instead of nitrogen). The identification of O-glycans shares many similarities with the identification of N-glycans, but there are also some key differences.

• • LC-MS Histone Modification Detection

  Liquid chromatography-mass spectrometry (LC-MS) is a powerful technique that is commonly used for the detection and quantification of biomolecules, particularly protein and other biomolecular modifications. Protein modifications, such as acetylation, methylation, phosphorylation, ubiquitination, and other types of modifications, are important epigenetic phenomena that occur on amino acid residues of histones.

• • Mycoplasma Detection in Recombinant Protein Vaccines

  Recombinant protein vaccines are vaccines prepared using genetic engineering techniques. The preparation process typically involves selecting one or more protein fragments from the target pathogen and inserting their genes into another organism, such as bacteria or yeast, for large-scale expression of these proteins. These recombinant proteins can then be purified and used as the main components of the vaccine.

• • Circular Dichroism Detection: A New Perspective on Proteins

  Proteins are important molecules in organisms, and their structure and function play a crucial role in life science. Circular Dichroism Spectroscopy (CD) serves as a highly specialized analytical tool, providing us with a fresh perspective to study and understand the structure, conformation, and interactions of proteins.

• • Interpreting of Circular Dichroism in Protein Structure Analysis

  Circular dichroism spectroscopy (CD) is a commonly used spectroscopic technique, primarily used for studying the secondary structure of proteins and other large molecules. CD spectroscopy detects the difference in absorption between left-handed circularly polarized light and right-handed circularly polarized light as they pass through the protein sample, which is caused by the sample's chirality. The CD spectrum of a protein is typically recorded in the wavelength range of 190-250 nm.

• • In-Depth Guide to CD: Techniques, Steps, and Data Analysis

  Circular dichroism spectroscopy (CD) is a spectroscopic technique commonly used to study the secondary structure of proteins and other biomolecules, such as α-helices, β-sheets, and random coils. CD spectra provide important information about molecular conformation and changes, particularly under varying external conditions like temperature, pH, and solvent environment.

• • Amino Acid Sequence Analysis Guide: Basics, Methods, and Practice

  The amino acid sequence refers to the arrangement of amino acids in a protein. This sequence is determined by genetic information, specifically through the process of transcription and translation of genes in DNA. Understanding amino acid sequences is important in biological research and fields like medicine, as they determine the structure and function of proteins.

• • Mechanisms of SEC and RPLC in Protein Purity Analysis

  In contemporary biochemistry and molecular biology research, protein purity analysis is indispensable. To guarantee the accuracy of subsequent experiments, such as structural determination, functional assays, or drug development, it is imperative to assess the purity of protein samples comprehensively.

• • Mechanism of HPLC-Based Protein Purity Detection

  Proteins are central molecules in life science research, and their purity directly impacts the reliability and reproducibility of experimental results. To ensure the purity of protein samples used in experiments, scientists have developed various analytical methods. Among these, High-Performance Liquid Chromatography (HPLC) has emerged as a crucial tool for protein purity detection, owing to its high resolution, sensitivity, and rapid analysis capabilities.