Resources
Proteomics Databases
Metabolomics Databases
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Biotin Proximity Labeling is a highly effective technique extensively employed in proteomics research to map protein-protein interactions, spatial organization, and dynamic protein behavior. At the core of this approach is the utilization of specialized enzymes, such as biotin ligase BirA or its enhanced variant TurboID, which covalently attach biotin molecules to lysine residues on proteins within their immediate proximity. These enzymes are typically genetically fused with target proteins and direct......
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HCP Characterization is a critical quality control process in biopharmaceutical manufacturing. HCPs refer to residual proteins unintentionally retained from host cell systems, including microbial hosts (e.g., bacteria, yeast) and mammalian cell lines, during the production of therapeutic proteins and biologics. The presence of HCPs can pose immunogenicity and toxicity risks, potentially compromising drug safety and therapeutic efficacy. Therefore, HCP characterization is an essential step throughout d......
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• Hybridoma Antibody Sequencing
Hybridoma antibody sequencing is an advanced analytical technique used to determine the amino acid sequences of antibodies produced by hybridoma cell lines. Hybridoma cells, formed by fusing B lymphocytes with myeloma cells, are capable of continuously producing monoclonal antibodies. In antibody drug development, hybridoma antibody sequencing is widely applied to optimize antibodies for improved stability, efficacy, and safety. By accurately determining the antibody sequence, researchers can gain a......
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• Protein Quantification Mass Spectrometry
Protein quantification mass spectrometry is a powerful analytical technique designed to measure protein levels in complex biological samples with high precision and accuracy. By integrating the sensitivity and specificity of mass spectrometry, it has become an essential tool in modern proteomics research. This technology has extensive applications, including biomarker discovery, disease mechanism elucidation, drug target identification, and the dynamic monitoring of biological processes. In protein ......
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• Edman Sequencing Service in Protein Analysis
Edman sequencing service is a specialized analytical technique used for determining the N-terminal amino acid sequence of proteins with high precision. Named after its inventor, Pehr Edman, this method employs Edman degradation to sequentially cleave amino acids from the N-terminus of a peptide, enabling their identification and quantification. This approach remains one of the most reliable tools for analyzing short peptide sequences, offering valuable insights into protein structure and functional ch......
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mRNA profiling is a high-throughput technique used to comprehensively analyze the expression patterns of all mRNA molecules in cells or tissues. By extracting, quantifying, and analyzing mRNA, this technique reveals gene expression dynamics and regulatory mechanisms under specific physiological or pathological conditions. It serves as a cornerstone in transcriptomics research, offering critical insights into gene regulation, disease pathology, drug target discovery, and precision medicine. Beyond id......
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Blood proteomics is an advanced analytical approach focused on investigating the types, structures, functions, and interactions of proteins in the blood. By integrating proteomics and mass spectrometry analysis, this high-throughput technique aims to map the intricate protein networks within biological systems. Blood, as a highly complex biological sample, serves as a reservoir of physiological and pathological information from multiple organ systems, positioning it as a critical focus in proteomics r......
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• De Novo Protein Sequencing Applications and Challenges
De novo protein sequencing is a method used to determine the primary structure of proteins directly from experimental data without relying on genomic or protein database references. In proteomics research, de novo protein sequencing plays a crucial role, especially in identifying novel or post-translationally modified proteins from non-model organisms or unknown protein sources, offering indispensable insights. Unlike traditional protein identification methods, which depend on pre-existing protein dat......
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mRNA characterization involves the systematic analysis of the structure, sequence, modifications, and functional properties of messenger RNA (mRNA) using advanced analytical technologies. This process typically encompasses mRNA extraction, quantification, qualitative analysis, and translation efficiency evaluation. As a central intermediary in gene expression, mRNA serves to translate genetic instructions from DNA into proteins, playing a critical role in cellular regulation, organismal development, a......
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Mass spec protein analysis is a powerful method for protein identification, quantification, and structural characterization using mass spectrometry. This technique separates and detects molecules in a sample based on their mass-to-charge ratio (m/z). The typical workflow of mass spec protein analysis begins with sample preprocessing, including protein extraction and enzymatic digestion. The resulting peptides are then separated using techniques such as liquid chromatography (LC) or electrophoresis bef......
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