Resources
Proteomics Databases
Metabolomics Databases
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• Single-Cell Protein Sequencing
Single-cell protein sequencing, or single-cell proteomics sequencing, is a technique for analyzing the proteome at the level of individual cells. Unlike conventional proteomics, which typically investigates large cell populations, this approach allows for the examination of protein expression and variation on a per-cell basis, thereby revealing cellular heterogeneity. The applications of single-cell protein sequencing are extensive, spanning from fundamental research into biological processes like cel......
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• Mass Spectrometry Used for Protein Identification
Mass spectrometry used for protein identification is an analytical technique used for the identification and characterization of proteins. Utilizing a mass spectrometer, researchers are able to measure the mass of protein molecules, analyzing them based on their mass-to-charge ratio (m/z). This technique is extensively applied in proteomics for its critical role in aiding scientists to gain profound insights into the functions and dynamic changes within organisms. The applications of mass spectrometry......
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• MS/MS Protein Identification
MS/MS protein identification is a technique that utilizes tandem mass spectrometry to analyze and identify proteins. Through this method, scientists can perform an in-depth analysis of the protein composition and structure within a sample. MS/MS protein identification operates by measuring the mass-to-charge ratio of peptides and decoding these features for identification by matching against databases, thereby determining the protein identities. This technique finds extensive application in basic rese......
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• Protein Purification and Characterization Techniques
Protein purification and characterization techniques are essential steps in biochemical and molecular biology research. These techniques aim to isolate, purify, and determine the structural and functional characteristics of proteins, facilitating a deeper understanding of their biological roles and potential applications. Proteins, as fundamental components of life, perform diverse functions in organisms, including catalysis of chemical reactions, signal transmission, molecular transport, and cellular......
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• Pull-Down Protein Interaction
Pull-down protein interaction is an experimental technique used to detect physical interactions between proteins. In life sciences, proteins are the primary molecules responsible for executing virtually all cellular functions. Protein-protein interactions are critical in numerous biological processes, including signal transduction, metabolic regulation, and gene expression control. Through pull-down protein interaction analysis, scientists can identify and verify contacts and functional connections be......
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MS/MS protein sequencing, or tandem mass spectrometry protein sequencing, is used to determine protein sequences. This technique supports proteomics research by identifying and quantifying proteins in complex biological samples, aiding in the understanding of biological system functions and mechanisms. The core principle involves ionizing proteins with a mass spectrometer and analyzing their mass-to-charge ratio in a vacuum. Using this method, researchers can resolve the primary structure of proteins ......
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Mass spec sequencing is a method used to determine the sequences of biological macromolecules, such as proteins and nucleic acids, by measuring their molecular mass. This technique enhances our understanding of biomolecular functions. The core principle involves ionizing the sample before analysis with a mass spectrometer, where ions are examined based on their mass-to-charge ratio (m/z). In proteomics, mass spec sequencing facilitates the identification of protein amino acid sequences, which aids in ......
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• Mascot Protein Identification
Mascot protein identification is a widely used bioinformatics method in the field of proteomics, combining mass spectrometry technology with database searches to identify proteins by analyzing the mass data of peptides. As a commercial software tool, Mascot plays a crucial role in proteomics research, enabling researchers to quickly and accurately identify proteins within complex biological samples. In proteomics research, protein identification is a key step in elucidating the protein composition of ......
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• How to Determine Protein Molecular Weight Effectively?
How to determine protein molecular weight effectively? Protein molecular weight determination not only helps to define the basic physicochemical properties of proteins but also plays a key role in verifying protein structure, assessing sample purity, and studying protein complex assembly states. In fields such as biopharmaceuticals, protein engineering, genetic engineering, and disease research, precise protein molecular weight determination is crucial for both scientific research and industrial appli......
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LC-ESI-MS/MS proteomics is extensively employed to investigate the protein composition, structure, and function in biological samples. This technique integrates the benefits of liquid chromatography and mass spectrometry, facilitating the efficient separation, detection, and identification of diverse proteins. Liquid chromatography effectively separates mixture components, enhancing the precision of subsequent mass spectrometric analysis. Electrospray ionization, a method for ionizing proteins for mas......
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