Resources
Proteomics Databases
Metabolomics Databases
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• Overview of Phosphoproteomics Analysis
Phosphoproteomics analysis is a pivotal technique for investigating protein phosphorylation and its regulatory roles in biological systems. As one of the most prevalent post-translational modifications, phosphorylation governs essential cellular processes, including signal transduction, metabolism, cell cycle progression, gene expression, and apoptosis. By systematically profiling phosphorylated proteins, researchers gain insights into cellular responses to external stimuli, regulatory mechanisms unde......
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TMT quantitative proteomics is a highly efficient protein quantification technique widely utilized in biological and medical research. By precisely quantifying protein abundance across samples, it reveals molecular changes within complex biological systems. This approach employs chemical labeling reagents to tag peptides from different samples with distinct isobaric mass tags, enabling simultaneous identification and quantification via mass spectrometry (MS). Compared to conventional protein quantific......
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• 2D-DIGE in Proteomics Analysis
Two-dimensional difference gel electrophoresis (2D-DIGE) is a widely employed technique in proteomics, designed to efficiently and accurately compare protein profiles across different samples. By combining two electrophoretic principles—Isoelectric Focusing (IEF) and Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE)—this method allows simultaneous analysis of multiple protein samples on the same gel, enabling high-resolution separation and quantitative analysis. 2D-DIGE is particula......
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Olink analysis is a high-throughput immunoassay platform technology designed for rapid and precise protein quantification. Leveraging Olink's unique Proximity Extension Assay (PEA) technology, researchers can simultaneously detect hundreds to thousands of protein biomarkers, significantly accelerating biomarker discovery and validation. PEA employs two antibodies that bind to the target protein, one carrying an extension probe. Upon binding, the probes form a complete DNA sequence, which is then detec......
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• Glycan Analysis of Monoclonal Antibody
The glycan analysis of monoclonal antibody is a crucial research area in biopharmaceuticals and biotechnology. Monoclonal antibodies are highly specific therapeutic proteins widely used to treat diseases such as cancer, immune disorders, and infectious diseases. This analysis focuses on detailed characterization of glycosylation patterns, a type of post-translational modification present in both the constant and variable regions of antibodies. Glycosylation profoundly influences antibody structure, st......
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Label-Free Quantification is a widely used technique in proteomics that directly measures the relative abundance of proteins in a sample using mass spectrometry, eliminating the need for chemical or isotope labels. This approach relies on detecting and comparing signal intensities across different conditions to infer protein abundance. Its simplicity and versatility have made it an essential tool across biomedical research, drug development, disease mechanism studies, and agricultural science. In bi......
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To identify protein is to accurately detect and characterize specific proteins within biological samples using advanced technical methodologies. The ability to identify protein is fundamental to understanding their biological functions, as protein activity and function are intrinsically tied to their structure and molecular interactions. This process is not merely about confirming the presence of proteins but also serves as the basis for comprehending their biological roles within cellular contexts. A......
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• Edman Degradation Sequencing
Edman degradation sequencing is a classic method used to determine the primary structure of proteins. This technique chemically modifies the N-terminal amino acid of a protein, enabling its stepwise removal from the N-terminus. This process generates a series of identifiable derivatives, facilitating the analysis of protein sequences. Specifically, the method involves the reaction of an isothiocyanate acyl derivative with the N-terminal amino acid of the polypeptide chain, producing a phenylthiohydant......
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Protein Quality Assessment is a critical technique in modern biological and medical research, designed to evaluate the purity, integrity, functionality, and biocompatibility of protein samples. This process is essential for ensuring that protein components in biologics, drug development, and the food industry meet the required standards. Protein quality assessment is not limited to biopharmaceutical applications but is also widely used in food safety testing, nutritional research, environmental monito......
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Top-down mass spectrometry is a technique used in proteomics to analyze intact proteins directly, without the need to cleave them into peptides, as in the traditional "bottom-up" approach. This method significantly enhances the resolution and accuracy of proteomic studies, especially in the investigation of post-translational modifications, polymorphisms, and isoforms of proteins. Top-down mass spectrometry provides detailed structural information about proteins, including the precise locations and ty......
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