Resources

Proteomics Databases



Metabolomics Databases

• • Principle of Glycan Profile Analysis Based on MALDI-TOF/LC-MS

  Glycan profiling is a crucial bioanalytical technique extensively used in biomedical research, particularly in the study of protein glycosylation. Glycosylation, an essential post-translational modification, significantly impacts protein structure, function, and biological behavior.

• • Application of Glycosylation Site Analysis in Protein Function Studies

  Glycosylation is one of the most common and complex forms of post-translational modifications (PTMs) and is widely found in mammals, plants, and microorganisms. By attaching sugar moieties to specific amino acid side chains, glycosylation modulates protein functions such as stability, activity, localization, and interactions with other molecules.

• • Workflow of Glycosylation Site Identification via Enzymatic Digestion and LC-MS/MS

  Glycosylation is a specific type of post-translational modification of proteins, playing a crucial role in numerous biological processes such as cell signaling, protein folding, and immune responses. Identifying the exact glycosylation sites on proteins is critical for advancing our understanding of these processes. Enzymatic digestion combined with LC-MS/MS (liquid chromatography-tandem mass spectrometry) is currently one of the most widely used methods for glycosylation site identification.

• • Advantages and Disadvantages of Glycosylation Site Analysis Using Enzyme Digestion and 18O Labeling

  Glycosylation is a crucial post-translational modification of proteins, affecting protein stability, function, and cell interactions. Accurate analysis of glycosylation sites is essential for understanding their role in biological processes. Among the various glycosylation analysis methods, enzymatic digestion and 18O labeling have been widely used for glycosylation site analysis due to their high accuracy and sensitivity.

• • Principle of Glycosylation Site Analysis Based on LC-MS/MS

  Glycosylation is a common and significant post-translational modification (PTM) of proteins, playing a critical role in cellular communication, immune response, protein folding, and disease progression. The study of glycosylation is crucial for understanding the pathological mechanisms of various disorders, such as cancer, metabolic dysfunction, and neurodegenerative diseases.

• • Mechanism of Glycoprotein Structural Analysis via Mass Spectrometry

  Glycoproteins are proteins covalently attached to one or more carbohydrate chains. They are widely present on the cell surface and in secreted proteins, playing vital roles in cell recognition, signal transduction, and immune response. Due to the complexity and diversity of glycan structures, elucidating glycoprotein structures is crucial for understanding their biological functions.

• • Application of Mass Spectrometry in Glycoprotein Analysis

  Glycoproteins are essential biomolecules involved in various biological processes, including cell communication, immune responses, protein folding, and molecular recognition. Glycosylation, a key post-translational modification, significantly influences the function and stability of glycoproteins. Hence, precise and sensitive analysis of glycosylation is crucial to understanding the biological roles of glycoproteins.

• • Workflow of Glycoprotein Detection Using LC-MS

  Glycoproteins play a critical role in various biological processes, including cell recognition, signal transduction, and immune responses, primarily due to their glycosylation modifications. Studying these glycosylation patterns can help elucidate molecular mechanisms behind diseases such as cancer and immune disorders. LC-MS (Liquid Chromatography-Mass Spectrometry) is a widely used technique for detecting and characterizing glycoproteins' glycosylation modifications.

• • Advantages and Disadvantages of Glycoprotein Analysis Methods

  Glycoproteins are a class of proteins that are widely present in living organisms and play essential roles in biological processes such as protein regulation, cell communication, and immune responses through glycosylation. Changes in glycosylation are often associated with various diseases, including cancer and metabolic disorders.

• • Principle of Glycoprotein Analysis Based on Mass Spectrometry

  Glycoproteins are biomolecules where proteins are covalently bonded with carbohydrate chains, playing vital roles in cellular recognition, signal transduction, immune regulation, and other biological processes. Glycosylation, one of the most complex post-translational modifications, significantly affects the structure, stability, and function of proteins. Hence, accurate analysis of glycoproteins is critical for understanding biological processes and developing disease biomarkers.