Resources

Proteomics Databases



Metabolomics Databases

• • Analysis of Protein Molecular Weight by Light Scattering

  Determining the molecular weight of proteins is fundamental in biochemistry and molecular biology, contributing to our understanding of protein structure, function, and interactions. Light scattering, a powerful and non-invasive analytical technique, offers precise measurements of protein molecular weight.

• • Analysis of Protein Molecular Weight by SDS-PAGE

  Protein molecular weight determination is fundamental in proteomics, providing insights into protein structure, function, and interactions. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) is a widely used method for analyzing protein molecular weight.

• • Detection and Analysis of Protein Interactions Based on MS

  Proteins are crucial for almost all biological processes, acting as the primary molecules that drive various life activities. Understanding protein-protein interactions is essential for uncovering the mechanisms underlying these biological processes and the pathologies of diseases. In recent years, mass spectrometry (MS) has emerged as a vital tool for studying protein-protein interactions due to its high sensitivity, throughput, and specificity.

• • Procedure for Protein Identification Using Tandem MS

  Proteins are fundamental molecular units of life, and understanding their structure and function is crucial for biological research. Mass spectrometry (MS) is an efficient analytical tool widely used in proteomics. Specifically, tandem mass spectrometry (MS/MS) provides detailed information about proteins and their peptides, significantly advancing the process of protein identification. This article details the procedure of protein identification using tandem mass spectrometry.

• • Mechanism of N-Glycan Relative Quantification via 2-AA Labeling

  N-glycosylation is a fundamental post-translational modification in eukaryotes and some prokaryotes, involved in key biological processes such as protein folding, stability, cell signaling, and immune response. To better understand the variation of N-glycans under different conditions, relative quantification techniques are essential. 2-Aminobenzoic acid (2-AA) labeling is a widely used method for this purpose.

• • Protein Characterization Based on WB

  Proteins are among the most crucial biomolecules in living organisms, responsible for executing various key functions. To understand protein functions, structures, and their roles in biological processes, researchers have developed various techniques for protein characterization. Among these, Western Blotting is a widely used and powerful technique.

• • Application of MALDI-TOF/LC-MS in N-Glycan Type Detection

  Glycosylation, a widespread post-translational modification (PTM) in eukaryotic proteins, plays a crucial role in protein function, stability, and cell communication. Among the various types of glycosylation, N-glycosylation is particularly important. Studying N-glycan structures and types is essential for understanding diseases such as cancer and autoimmune disorders, as well as for developing biomarkers.

• • Workflow of N-Glycan Profiling by PNGase F Digestion and 2-AA Labeling

  N-glycan profiling is a widely used technique in biological research and clinical applications to elucidate glycosylation patterns of glycoproteins. This method is crucial for understanding cellular processes, disease mechanisms, and the discovery of biomarkers. By digesting glycoproteins with PNGase F (Peptide-N-Glycosidase F) to release N-glycans and labeling them with 2-AA (2-Aminobenzoic acid), researchers can effectively analyze glycan structures.

• • Advantages and Disadvantages of Glycan Profiling Using MALDI-TOF/LC-MS

  Glycan profiling is a crucial step in understanding the structure and function of glycans. In recent years, mass spectrometry-based analytical techniques such as MALDI-TOF (Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry) and LC-MS (Liquid Chromatography-Mass Spectrometry) have played a pivotal role in glycan analysis. These technologies provide high sensitivity and throughput for glycan analysis, but each has its own advantages and limitations.

• • Mechanism of Glycan Release in Glycosylation Site Detection Using 18O Labeling

  Glycosylation is a crucial form of post-translational modification in proteins, widely present in cell surface and secreted proteins. Understanding glycosylation is vital for elucidating protein function, cellular signaling, and disease progression. One of the primary focuses of glycosylation research is the site-specific identification of glycan-modified locations.