Resources

Proteomics Databases



Metabolomics Databases

• • Edman Protein Sequencing

  Edman protein sequencing is a precise method for determining the amino acid sequence of proteins by sequentially identifying the N-terminal residues of a polypeptide chain, thereby establishing the protein's primary structure. Introduced by Pehr Edman in 1950, it has since become a cornerstone in biochemical and molecular biology research. The technique relies on the Edman degradation reaction, where the N-terminal amino acid of the protein or polypeptide reacts with phenylisothiocyanate (PITC) to for......

• • Size-Exclusion Chromatography Protein Purification

  Size-exclusion chromatography protein purification is a technique used to separate and purify proteins based on their differences in molecular size. It utilizes porous materials packed within a column, functioning as a sieve that allows components of a mixture to pass through at varying rates, depending on their size, leading to separation. The core principle of this technique is the volume exclusion effect, where larger molecules cannot enter the pores or can only enter larger pores, causing them to ......

• • Size Exclusion Chromatography for Protein Purification

  Size exclusion chromatography for protein purification is a widely utilized method in biochemical and proteomics research, allowing the separation of molecules based on differences in size and shape. This technique is essential for ensuring the accuracy and reliability of subsequent analyses, particularly in proteomics. Also known as gel filtration chromatography, the method uses columns packed with porous beads to separate proteins in a mixture. Larger molecules are excluded from the bead pores and e......

• • Antibody Profiling

  Antibody profiling refers to the detailed study and characterization of the structure, function, and properties of antibodies. As essential components of the immune system, antibodies specifically recognize and bind to antigens, thereby contributing to immune defense. In biomedicine, antibodies are used not only as diagnostic tools but also for the treatment of various diseases, including cancer, autoimmune disorders, and infections. Consequently, antibody profiling plays a pivotal role in drug develo......

• • Protein Sequence Analysis Overview

  Protein sequence analysis involves determining the amino acid sequence and chemical modifications of proteins using advanced methodologies. Proteins are essential macromolecules that adopt complex three-dimensional structures derived from their linear amino acid sequences, enabling them to perform diverse cellular functions. The primary objective of protein sequence analysis is to elucidate the primary structure of proteins, providing critical insights into their function, structure, and interactions.......

• • Protein Sequencing by Mass Spectrometry

  Protein sequencing by mass spectrometry is a powerful method for determining the amino acid sequence of proteins. Proteins play central roles in biological processes, with their structure and function directly influencing an organism's growth, development, and metabolism. Understanding the amino acid sequence of proteins provides crucial insights into their functional mechanisms. Traditional methods such as Edman degradation, while accurate, have been largely supplanted by mass spectrometry-based tech......

• • Protein Characterization Techniques

  Protein characterization techniques comprise a diverse array of methodologies that facilitate in-depth understanding of protein structure, function, and interactions. Key techniques include mass spectrometry, nuclear magnetic resonance (NMR) spectroscopy, X-ray crystallography, circular dichroism (CD), and cryo-electron microscopy (Cryo-EM). Mass spectrometry is indispensable in the identification and quantification of proteins, particularly within proteomics, enabling the rapid and precise analysis o......

• • Protein Purification Size Exclusion Chromatography

  Protein purification size exclusion chromatography (SEC), also known as gel filtration chromatography, is a technique widely used to separate proteins and other biological macromolecules based on their size and shape. In proteomics, precise separation and purification of target proteins are essential for elucidating their structure and function. SEC relies on a porous medium packed within a chromatography column, allowing molecules to traverse different pathways based on their size. Smaller molecules ......

• • Peptide Content Determination

  Peptide content determination refers to the precise measurement of peptide levels in a sample using scientific methods. This process allows researchers to accurately assess the concentration and distribution of peptides in a sample, providing vital data for product quality control, clinical treatment planning, and new drug development. As fragments of proteins, peptides possess unique biological activities and are commonly used in early disease diagnosis, therapeutic drug development, and functional i......

• • Protein Characterization Methods

  Protein characterization methods encompass a range of techniques, including mass spectrometry, electrophoresis, X-ray crystallography, and nuclear magnetic resonance (NMR) spectroscopy. These methods are essential for understanding protein structure and function in various biological contexts. Mass spectrometry enables precise determination of protein molecular weight and structural features, and is widely utilized in proteomics research. Electrophoresis, on the other hand, is a common method for prot......