Resources

Proteomics Databases



Metabolomics Databases

• • Protein Characterization Based on MS

  Proteins play crucial roles in life processes, and studying their structure and function is vital for understanding biological mechanisms. Mass spectrometry (MS) is a powerful analytical technique essential for protein characterization.

• • Protein Characterization Based on Nuclear Magnetic Resonance

  Proteins play a critical role in biological processes, and their structure is key to their function. Determining the three-dimensional structure of proteins is vital for understanding their biological roles. Nuclear Magnetic Resonance (NMR) is a powerful technique extensively used for protein structural characterization.

• • Identification of Protein Complexes via MS

  Protein complexes play crucial roles in cellular processes, including signal transduction, metabolic pathways, and gene expression regulation. Identifying and analyzing these complexes is essential for understanding cellular functions and disease mechanisms. Mass spectrometry (MS), a powerful analytical tool, has been widely employed in studying protein complexes.

• • Analysis of Protein Complexes using MALDI-TOF-MS and ESI-MS

  Mass spectrometry (MS) plays a crucial role in proteomics research, particularly in analyzing the composition and structure of protein complexes. Two commonly used MS techniques are Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF-MS) and Electrospray Ionization Mass Spectrometry (ESI-MS).

• • Application of Glycomic Profiling in Therapeutic Glycoprotein Production

  Glycomics is the study of the structure, function, and interactions of carbohydrates within biological systems. Glycoproteins, which play critical roles in immune regulation, intercellular signaling, and protein function modulation, are a key class of biomolecules. Therapeutic glycoproteins, including antibodies, hormones, and cytokines, are widely employed in modern biopharmaceuticals. However, the production of glycoproteins is challenging, particularly due to the complexity of glycosylation.

• • Workflow of Glycomic Profiling via Hydrophilic Chromatography

  Glycomics is the study of the types, structures, and functions of sugar molecules in biological systems. These molecules play a critical role in cell recognition, signal transduction, and immune responses. However, due to the heterogeneity and complexity of glycans, glycomics research faces numerous technical challenges. Hydrophilic Interaction Chromatography (HILIC) has recently emerged as an essential tool in glycomics due to its excellent separation capabilities and retention of polar molecules.

• • Advantages and Disadvantages of Glycomic Profiling Using MALDI-TOF-MS

  Glycomics, the study of glycans and their interactions, is critical for understanding biological processes and discovering disease biomarkers. Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS) is a versatile analytical technique that has gained prominence in glycomics due to its rapid and sensitive detection capabilities.

• • Principle of Glycomic Profiling via N-glycan Analysis

  Glycomics, the study of the structure and function of glycans across cells, tissues, and organisms, is a field of increasing importance in biological research. Glycans are among the most structurally complex biological macromolecules, appearing in proteins, lipids, and other biomolecules, and are involved in essential processes such as cell recognition, signal transduction, and immune responses.

• • Mechanism of Electron Transfer Dissociation in Top-Down Proteomics for PTMs

  Proteomics is an essential field for studying the composition and dynamics of proteins in cells, tissues, and organisms. In recent years, top-down proteomics has gained considerable attention because of its ability to retain complete protein information. Unlike bottom-up approaches, which digest proteins into smaller peptides, top-down proteomics analyzes intact proteins, providing crucial insights into their structures and post-translational modifications (PTMs).

• • Application of Top-Down Mass Spectrometry for PTMs Detection

  Post-translational modifications (PTMs) refer to the covalent modifications of proteins after translation, altering their structure and function. PTMs, including acetylation, phosphorylation, methylation, ubiquitination, and others, regulate various physiological functions such as cell signaling, protein degradation, and gene expression. However, due to the complexity and diversity of PTMs, detecting and analyzing these modifications effectively poses a significant challenge.