Resources

Proteomics Databases



Metabolomics Databases

• • MALDI Protein Analysis

  MALDI protein analysis is an advanced method based on mass spectrometry that employs a laser as an energy source to ionize proteins from solid to gaseous states via matrix molecule absorption and transfer. This technique is highly valued in proteomics for its minimal sample requirements, rapid analysis, and high sensitivity, making it indispensable for determining protein molecular weight, analyzing structures, and identifying samples. MALDI protein analysis is instrumental in biomedicine, drug develo......

• • Label-Free Quantitative Mass Spectrometry

  Label-free quantitative mass spectrometry (LFQ) is a powerful tool in proteomics research, enabling the direct analysis of relative protein abundance changes in samples without requiring exogenous labeling. This method offers significant advantages, including high throughput and cost efficiency, while preserving the intrinsic properties of proteins during analysis. By eliminating labeling steps, LFQ simplifies experimental workflows and minimizes associated errors and complexities. Furthermore, LFQ fa......

• • Isobaric Labeling Mass Spectrometry

  Isobaric labeling mass spectrometry is a technique that introduces isotopic labels into specific biomolecules via chemical methods, facilitating quantitative and qualitative analysis using mass spectrometry. This approach finds broad applications in proteomics, particularly in the study of differential proteomics and dynamic protein changes. As biomedical research advances, the need for precise quantification and identification of proteins in complex biological systems is growing. By introducing known......

• • Label-Free Quantification Using MaxQuant

  Label-Free Quantification using MaxQuant is a mass spectrometry-based proteomics analysis method primarily utilized for the relative quantification of protein abundance. As the field of proteomics rapidly evolves, there is an increasing demand for precise and efficient methods to analyze complex protein mixtures. Traditional labeling techniques such as iTRAQ and SILAC, while providing accurate quantitative data, require complex chemical labeling of samples, which is both cumbersome and costly. In cont......

• • Identification of Post-Translational Modifications

  The identification of post-translational modifications (PTMs) represents a pivotal area in contemporary biological and biochemical research. PTMs refer to a series of chemical changes that proteins undergo following translation, including phosphorylation, acetylation, methylation, ubiquitination, and glycosylation. These alterations can profoundly influence protein function, stability, localization, and interactions with other molecules, thus playing a critical role in processes like cell signaling, m......

• • IPA Protein Analysis

  IPA protein analysis is a specialized software tool designed for the analysis and interpretation of complex biological data. It is extensively utilized in proteomics, genomics, and transcriptomics to assist scientists in understanding the functions and mechanisms of biological systems in greater depth. By leveraging the comprehensive data resources of the Ingenuity Knowledge Base, the software provides insights into gene-protein interactions, metabolic pathways, and signaling pathways. The primary fun......

• • GO Analysis Y-Axis

  In proteomics, GO (Gene Ontology) analysis is a method used to comprehensively describe gene attributes and the functions of gene products. In a GO analysis result graph, the x-axis typically represents GO terms, while the y-axis usually indicates the number of genes or the enrichment ratio. The interpretation of these axes is essential for understanding gene function and its role within an organism, and should be guided by the specific annotations provided in the graph. GO analysis is categorized into.....

• • ADC Structure Analysis

  Antibody-Drug Conjugates (ADCs) represent an innovative class of targeted therapeutic agents, merging the specificity of monoclonal antibodies with the toxicity of small-molecule drugs. The effective design and optimization of ADC structures are paramount for maximizing therapeutic benefits while ensuring safety. This article offers a comprehensive examination of the structural components of ADCs and their critical role in biological sciences.

• • What Aspects Does Conventional Proteomics Measure

  Proteomics is a discipline that studies the quality, structure and function of proteins. Conventional proteomics usually studies the following aspects: 1. Protein Quantification Protein quantification is a crucial aspect of proteomics research. Through quantitative protein analysis, we can assess protein abundance and gain insights into their physiological roles. 2. Protein-Protein Interaction Studying protein-protein interactions provides insights into protein functions within cells and their network......

• • Cell Sample Processing in Proteomics

  1. Cell Separation The initial step involves the separation of cells, typically achieved through mechanical or enzymatic methods. Mechanical separation primarily involves physical methods to dissociate cells from tissue, whereas enzymatic separation utilizes enzymes to degrade intercellular connections. 2. Cell Disruption Following separation, cells require disruption to facilitate the extraction of intracellular proteins. Methods for cell disruption include sonication, freeze-thaw cycles, and chemical.....