Resources

Proteomics Databases



Metabolomics Databases

• • Sequencing Protein Amino Acid Sequences by Mass Spectrometry

  In biological research, sequencing is commonly employed to ascertain the precise sequence of molecules such as DNA or RNA, while protein sequences are typically elucidated using mass spectrometry. In protein mass spectrometry, proteins are initially digested into smaller peptide fragments. The mass of these fragments is then measured to infer the original amino acid sequence of the protein.

• • How Much Tissue Is Needed for TMT Protein Detection

  When performing Tandem Mass Tag (TMT) protein detection, the required tissue mass is primarily determined by the abundance of the target protein and the sensitivity of the detection method. Typically, an initial protein concentration between 100 and 500 µg is used. However, if the target protein is highly abundant in the sample, the required tissue mass may be significantly lower.

• • What Is the Normal Range for Protein Quantification

  The normal range of proteins in human blood is primarily composed of two components: albumin and globulin. The specific reference ranges are detailed below: 1. Total Protein (TP): Reference range, 60–80 g/L 2. Albumin (ALB): Reference range, 35–55 g/L 3. Globulin (GLO): Reference range, 20–30 g/L 4. Albumin/Globulin Ratio (A/G): Reference range, 1.2–2.5

• • iTRAQ/TMT Labeled Peptide Segments

  The application of iTRAQ or TMT labeling technologies is a widely used approach in quantitative proteomics analysis. These methods are based on stable isotope labeling, where isotope tags are chemically introduced to the amino groups at the N-terminus and lysine (K) side chains of peptides. This modification assigns each peptide a distinct mass tag, which can be identified during mass spectrometry analysis. Both iTRAQ and TMT are chemical labeling techniques that enable isotopic labeling of peptides........

• • Protein Function Validation and PRM

  Protein Function Verification (PFV) is a fundamental task in biological sciences, aimed at determining the biological roles and mechanisms of proteins through experimental approaches. Parallel Reaction Monitoring (PRM), a mass spectrometry-based technique, is frequently employed to quantify protein expression levels and validate their functional properties. Introduction to PRM Parallel Reaction Monitoring (PRM) is a highly sensitive and quantitative mass spectrometry technique that employs precise mass.....

• • Can Proteomics Specifically Detect the Content of a Particular Protein

  Proteomics allows for the precise quantification of specific proteins. It is a multidisciplinary field that examines the expression, modification, interaction, and function of proteins. Proteomics involves comprehensive methods for the identification and quantification of proteins, including all those encoded by genes and their derivatives. Additionally, it explores how proteins influence cellular functions and biological processes. Mass spectrometry is typically employed in proteomics to assess protein....

• • Can the P-Value for Protein Differential Analysis Be Set to 0.2

  In the context of differential protein analysis, the p-value is a widely utilized metric for assessing statistical significance, indicating whether the observed discrepancies from the null hypothesis may have occurred by chance. While researchers have the autonomy to select any p-value as the significance threshold, a value of 0.05 or stricter is typically chosen in most scientific investigations. Selecting a higher p-value, such as 0.2, can elevate the risk of committing a Type I error, which involves.....

• • How to Create a Quantitative Proteomics Heatmap

  A quantitative proteomics heatmap is a visualization tool used to illustrate changes in protein abundance across different samples. Creating such a heatmap involves several steps, including data preparation, normalization, clustering analysis, and plotting. This tutorial provides a step-by-step guide for constructing such a heatmap. 1. Data Preparation To begin, a quantitative proteomics dataset is required. Typically, this dataset is organized in a tabular format, with columns representing samples, rows...

• • 10x RNA Sequencing

  10x RNA sequencing represents an advanced method of droplet-based single-cell RNA sequencing that captures gene expression profiles at single-cell resolution. By isolating individual cells and encapsulating them in oil droplets, this technology leverages unique barcode labeling alongside high-throughput sequencing to conduct transcriptome analysis at the single-cell level. The capabilities of this method allow researchers to deeply investigate cellular heterogeneity and interactions, thus facilitating......

• • Droplet-Based Single-Cell Sequencing

  Droplet-based single-cell sequencing is an innovative biotechnology that facilitates high-throughput analysis of complex biological samples at the single-cell level. This technique involves the encapsulation of individual cells into microdroplets, where nucleic acid amplification and sequencing reactions occur, allowing for efficient cell isolation and independent genetic analysis. In contrast to traditional bulk sequencing methods, droplet-based single-cell sequencing provides detailed insights into ......