Resources

Proteomics Databases



Metabolomics Databases

• • Principle of Quantitative Tyrosine Phosphoproteomics Analysis

  Tyrosine phosphorylation is a pivotal molecular event in intracellular signaling, regulating critical cellular processes such as proliferation, differentiation, metabolism, and survival. Controlled by tyrosine kinases and phosphatases, tyrosine phosphorylation plays a central role in understanding signal transduction pathways.

• • Detection of Antibodies Using PCR Based Sequencing

  Antibodies are essential protein molecules in the immune system that specifically recognize and neutralize foreign pathogens. Traditional antibody detection methods, such as Enzyme-Linked Immunosorbent Assay (ELISA) and Immunofluorescence Assay (IFA), are widely used but have limitations in sensitivity, specificity, and result interpretation.

• • Steps of De Novo Protein Sequencing Based on Mass Spectrometry

  De Novo protein sequencing involves deducing the amino acid sequence of a protein directly from mass spectrometry data without any reference sequence. This process is intricate and meticulous. 

• • Detection of Protein Mutations Using High-Resolution MS

  Protein mutations play a significant role in biological research. Mutations can lead to changes in protein function, affecting physiological and pathological processes in organisms. Traditional methods for detecting protein mutations include Sanger sequencing and next-generation sequencing (NGS), which, despite their high accuracy and sensitivity, have limitations in complex protein samples.

• • High-Resolution Mass Spectrometry-Based De Novo Peptide Sequencing

  As proteomics research progresses, understanding protein structures and functions becomes crucial for elucidating biological processes. In this context, peptide sequencing is a vital method for analyzing protein structures. Traditional peptide sequencing methods rely on database matching, which is limited when dealing with unknown protein sequences.

• • Nano-LC-Based Analysis of De Novo Peptide Sequencing

  Proteins play diverse roles in biological organisms, from enzymes catalyzing chemical reactions to structural components of cells. To fully understand the functions and structures of proteins, scientists have developed various techniques, among which De Novo peptide sequencing is crucial. De Novo peptide sequencing is a method that directly deduces peptide sequences from mass spectrometry data without the need for a reference database.

• • MS/MS-Based Detection Procedure of De Novo Peptide Sequencing

  Mass spectrometry (MS) technology is pivotal in proteomics research, particularly for peptide sequencing. MS/MS (Tandem Mass Spectrometry) serves as the primary tool for De Novo peptide sequencing due to its high sensitivity and resolution. De Novo sequencing derives peptide sequences directly from mass spectrometry data without relying on reference databases, making it an essential method for protein identification.

• • Sequence Verification in De Novo Antibody Sequencing

  Antibodies play a crucial role in biomedical research and therapy due to their specificity and affinity, making them ideal tools for diagnosing and treating various diseases. Accurate determination of their amino acid sequences is essential for further understanding and utilization. De Novo antibody sequencing, a technique independent of reference sequences, is gaining importance in antibody research.

• • Procedure of De Novo Antibody Sequencing Based on Orbitrap Fusion Lumos

  Antibodies are crucial components of the immune system and play vital roles in modern biomedical research and applications. Antibody sequencing is essential for revealing the amino acid sequence of antibodies, providing critical information for vaccine development, disease diagnosis, and treatment. Recently, the de novo antibody sequencing method using the Orbitrap Fusion Lumos mass spectrometer has demonstrated significant advantages in antibody sequence analysis.

• • Analysis of Accurate Mass Determination Using MS

  Mass spectrometry (MS) is a highly sensitive and specific analytical technique widely utilized in biological research. It enables precise mass measurement of compounds in complex samples, facilitating researchers in identifying, quantifying, and analyzing biomolecules.