Resources

• • Application of KEGG Pathway Analysis in Differential Protein Expression

  In proteomics, differential protein expression analysis has become a vital tool for understanding how biological systems respond and adapt to various environmental changes. KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway analysis, a widely used bioinformatics method, helps researchers gain insights into complex protein and gene interaction networks by providing comprehensive functional annotations and pathway information.

• • Mechanism of KEGG Pathway Enrichment Analysis

  Pathway enrichment analysis is widely utilized in biological research to understand the functional roles of genes or proteins in various biological processes. KEGG (Kyoto Encyclopedia of Genes and Genomes) is one of the most commonly used bioinformatics resources for pathway enrichment analysis. KEGG pathway enrichment analysis reveals the enrichment of specific biological pathways in a gene set by comparing gene expression differences under different experimental conditions.

• • Advantages and Limitations of KEGG Pathway Analysis

  The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis is one of the widely used tools in bioinformatics and genomics research. It provides a systematic approach for exploring the relationships between genes and proteins within biological pathways and for investigating their roles in various biological processes. Through KEGG pathway analysis, researchers can identify and interpret the functional roles of genes or proteins within complex biological networks.

• • Workflow of KEGG Pathway Annotation and Enrichment Analysis

  The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis is a widely-used tool in modern biological research, particularly in fields such as genomics, transcriptomics, and metabolomics. The KEGG database aggregates extensive information on genes, compounds, reactions, and their interactions, facilitating the biological annotation of omics data and providing insight into gene function relationships within networks.

• • Principle of KEGG Pathway Annotation and Enrichment Analysis

  In bioinformatics and systems biology, the Kyoto Encyclopedia of Genes and Genomes (KEGG) database is a major resource for the systematic study of biomolecular functions. The KEGG database contains extensive information on metabolic and signaling pathways, making it an essential tool for understanding molecular networks and gene functions.

• • The Role of Primary Structure in the Immunogenicity of Biopharmaceuticals

  Biological products are drugs or other biological preparations manufactured using biotechnology, widely used in disease treatment and health improvement. The primary structure plays a crucial role in the immunogenicity research of biological products. The primary structure refers to the amino acid sequence of proteins or polypeptides in biological products, which determines the structure and function of the biological products.

• • iTRAQ Quantitative Proteomics

  iTRAQ (Isobaric Tags for Relative and Absolute Quantitation) is an isotope tagging technology for relative and absolute quantitation, extensively used in proteomics research. iTRAQ technology marks proteins or peptides to achieve protein quantification analysis, thereby revealing the dynamic changes of proteins in biological processes.   The core of iTRAQ technology is to use isotope labeling reagents to label protein samples. These labeling reagents consist of two parts: the balance region and the re......

• • Optimizing Mass Spectrometry Quantification Techniques for Protein

  The technology of mass spectrometry detection of proteins has penetrated into the fields of biomedical, food detection, pharmaceutical analysis, etc., such as the analysis of protein content in drugs, or the discovery of disease biomarkers. However, the accuracy and efficiency of this method are still limited by many factors, such as sample processing, matrix effects, etc. Next, let's break down each step and delve into how to optimize the quantitative techniques for mass spectrometry detection of pro......

• • LC-MS Proteomics Technical Points From Sample Preparation to Data Analysis

  In the field of biological sciences, the study of proteomics has become an important means to explore the complexity of biological systems. Liquid Chromatography-Mass Spectrometry (LC-MS) is one of the core technologies of proteomics.   Sample Preparation Sample preparation is a critically important step in LC-MS proteomics, as it directly relates to the accuracy and reliability of subsequent analyses. High-quality sample preparation requires meticulous design and operation.

• • Optimizing Immunoprecipitation Experiments to Improve the Purity and Specificity of Peptide Samples

  Immunoprecipitation is an important experimental technique used in the field of biopharmaceuticals to study protein interactions. In this process, antibodies bind specifically to target proteins, and the complexes are then separated by precipitation techniques for further analysis. However, due to the complexity and specificity of the samples, immunoprecipitation experiments may face challenges such as low purity and non-specificity.