Resources

Proteomics Databases



Metabolomics Databases

• • Workflow of Protein Characterization Analysis

  Protein characterization analysis is a crucial step in biological research, providing detailed insights into the structure, function, and interactions of proteins. The workflow of protein characterization analysis includes sample preparation, separation and purification, mass spectrometry analysis, data processing, and functional validation.

• • Principle of Protein Characterization Analysis

  Protein characterization analysis plays a crucial role in biological research. By analyzing protein structure, function, dynamics, and interactions, we can uncover fundamental principles of biological processes and provide scientific evidence for disease treatment and drug development. This article systematically introduces the principles of protein characterization analysis in three main aspects: protein separation, identification, and functional analysis.

• • MS-Based Accurate Mass Determination Procedure

  Mass spectrometry (MS) is a highly sensitive and high-resolution analytical technique widely used in biological research. The Mass Spectrometry-based Accurate Mass Measurement (MS-AMM) technique determines the precise mass of molecular ions to infer their chemical composition and molecular structure, establishing itself as a core tool in modern biochemistry and molecular biology research.

• • Principle of Unknown Protein Identification

  Proteins play fundamental roles in life activities, with their diverse functions and structures underpinning various biological processes. Despite advances in proteomics, many proteins remain poorly understood. To address this, scientists employ a range of techniques to analyze these unknown proteins.

• • Application of Protein Molecular Weight Determination

  Proteins are the workhorses of biological systems, playing critical roles in virtually every cellular process. Understanding the molecular weight of proteins is fundamental to biochemistry and molecular biology, providing insights into their structure, function, and interactions. This article explores the importance, methodologies, and applications of protein molecular weight determination, blending scientific rigor with accessible explanations.

• • Mechanism of MS Based Protein Identification

  Mass spectrometry (MS) is a powerful analytical tool widely used in biological research. MS technology is essential for protein identification and quantification due to its high sensitivity, accuracy, and resolution. This article explores the mechanisms of mass spectrometry-based protein identification and introduces the latest advancements in this field.

• • Workflow of Protein Molecular Weight Determination by MS

  Proteins, the workhorses of the cell, play crucial roles in virtually all biological processes. Understanding their molecular weight is fundamental in characterizing their structure and function. Mass spectrometry (MS) has emerged as a pivotal tool in this regard, offering unmatched sensitivity and accuracy. This article outlines the standard workflow for determining protein molecular weight using MS, bridging the gap between complex scientific methodology and comprehensible exposition.

• • Application of MS Based Protein Identification

  Mass spectrometry (MS) is a powerful and highly sensitive analytical tool widely used in proteomics research. Mass spectrometry-based protein identification (MSPI), a critical branch of MS technology, has become one of the core techniques in modern biological research. This article will detail the basic principles of MSPI and its applications in various fields.

• • Advantages and Disadvantages of Protein Molecular Weight Determi

  The molecular weight of a protein is the sum of the relative atomic masses of all the atoms in its chemical formula. Accurate determination of protein molecular weight is essential for structural analysis, functional studies, and biopharmaceutical development. Commonly used methods include viscosity measurements, gel permeation chromatography (GPC), SDS-PAGE, light scattering, and mass spectrometry (MS).

• • Workflow of MS Based Protein Identification

  Mass spectrometry-based protein identification is a sophisticated and precise method used to identify and quantify proteins in a sample. By leveraging mass spectrometry technology, researchers can gain deep insights into the structural and functional properties of proteins, thus elucidating the dynamic changes in biological systems.