Resources

Proteomics Databases



Metabolomics Databases

• • Protein Sulfonic Acid Modification Detection

  Protein sulfonic acid modification is a less common but very important kind of protein post-translational modifications. It involves the addition of a sulfate group to specific amino acid residues of a protein, usually tyrosine residues. Unlike phosphorylation, sulfonation is a covalent, permanent modification.   Common methods for detecting protein sulfonation include: 1. Mass Spectrometry (MS) LC-MS/MS can be used to identify sulfonation sites due to the mass increase caused by sulfonation.

• • Post-Translational Modification Proteomics

  Post-Translational Modification Proteomics (abbreviated as PTM Proteomics) is a subfield of proteomics that specifically studies the types, locations and dynamic changes of protein post-translational modifications. Since post-translational modifications have a key impact on protein function, activity, location and stability, in-depth research on it is of great significance for understanding cellular signal transduction, protein network interactions and disease onset mechanisms.

• • Mechanisms of iTRAQ, TMT, and SILAC in Protein Quantification

  With the rapid advancement of proteomics, quantitative proteomics techniques have been widely applied in biological research. iTRAQ (Isobaric Tags for Relative and Absolute Quantitation), TMT (Tandem Mass Tags), and SILAC (Stable Isotope Labeling by Amino Acids in Cell Culture) are three commonly used methods for protein quantification, each with its unique advantages and applications.

• • Applications of iTRAQ, TMT, and SILAC in Protein Quantification

  In proteomics research, the precise quantification of proteins in complex biological samples is a critical objective. To achieve this, several labeling technologies have been developed, including iTRAQ (Isobaric Tags for Relative and Absolute Quantification), TMT (Tandem Mass Tags), and SILAC (Stable Isotope Labeling with Amino Acids in Cell Culture).

• • Workflows of iTRAQ, TMT, and SILAC in Protein Quantification

  Protein quantification is crucial for understanding cellular functions, disease mechanisms, and biological system complexities in proteomics research. iTRAQ (Isobaric Tags for Relative and Absolute Quantification), TMT (Tandem Mass Tag), and SILAC (Stable Isotope Labeling by Amino acids in Cell culture) are three widely used quantitative proteomics methods. Each method has unique advantages and is extensively applied in relative or absolute protein quantification.

• • Advantages and Disadvantages of iTRAQ, TMT, and SILAC in Protein Quantification

  In proteomics research, quantitative analysis is a crucial tool for uncovering protein expression levels, molecular regulatory mechanisms, and cellular signaling pathways. iTRAQ, TMT, and SILAC are three commonly used protein quantification techniques.

• • Principles of iTRAQ, TMT, and SILAC in Protein Quantification

  Protein quantification plays a crucial role in modern life sciences research. By quantifying the relative or absolute abundance of proteins, researchers can gain deep insights into changes in protein expression profiles under different conditions, and how these changes contribute to diseases, development, or environmental responses.

• • Analysis of Antibody Sequences Using Mass Spectrometry

  Antibodies play a crucial role in biomedical research and clinical applications. Accurate antibody sequence analysis is essential for antibody engineering, drug development, and immunotherapy. In recent years, mass spectrometry (MS) has become a powerful tool for antibody sequence analysis due to its high sensitivity, high resolution, and high throughput.

• • Procedure of Top-Down Protein Sequencing for Modified Terminals

  Top-down protein sequencing is a powerful mass spectrometry-based approach used to analyze intact proteins without the need for enzymatic digestion. This method provides a comprehensive view of protein modifications, including those at the N- and C-terminal ends, which are critical for understanding protein function and regulation.

• • Analysis of Monoclonal Antibodies Using De Novo Sequencing

  Monoclonal antibodies (mAbs) are antibodies produced by a single B cell clone, known for their high specificity and consistency. They have become crucial in therapeutic, diagnostic, and research applications.