Resources

Proteomics Databases



Metabolomics Databases

• • Hydrogen Deuterium Exchange Mass Spectrometry Results Analysis

  Hydrogen Deuterium Exchange Mass Spectrometry (HDX-MS) is a valuable technique for identifying key dynamic regions within biomolecules. By measuring the rate of hydrogen-deuterium exchange, HDX-MS provides insights into whether specific regions are exposed or interact with other molecular domains. Principle: The underlying principle of HDX-MS is the exchange reaction between hydrogen and deuterium, where a hydrogen atom in a biomolecule is substituted by a deuterium atom. This substitution is detectable....

• • How to Master Protein Identification for Precise Biomarker Discovery

  Protein identification technologies are essential for the discovery, validation, and application of biomarkers. Biomarkers are biomolecules that indicate biological process states, disease progression, or drug responses. Among them, proteins, due to their essential roles in biological processes, represent a biomarker class with significant potential for clinical translation. Precise biomarker analysis relies on efficient and accurate protein identification techniques to ensure that identified proteins......

• • Protein Identification: 12 Common Mistakes to Avoid

  Protein identification is a crucial step in proteomics research. By precisely analyzing and identifying proteins within a sample, scientists can elucidate the fundamental roles of proteins in cellular function, disease mechanisms, and drug development, among other areas of biological research. However, due to the technical complexity and sample variability, protein identification often presents significant challenges. In this article, we summarize 12 common mistakes encountered during protein .......

• • How to Accurately Identify Proteins? Key Methods and Experimental Strategies

  Accurate protein identification is an integral part of life science research, involving the precise recognition and analysis of proteins in biological systems. The core of accurately identify proteins lies in utilizing high-precision technologies to achieve efficient and accurate protein identification while optimizing experimental strategies to ensure data reliability and reproducibility. With the advancement of life science research and proteomics technologies, researchers continuously explore more ......

• • Comprehensive Guide to Protein Identification: Techniques, Applications, and Data Analysis

  Protein identification is an integral part of life sciences research, with broad applications in areas such as disease research, new drug development, biomarker screening, and synthetic biology. Accurate protein identification depends on the use of advanced technologies, systematic experimental design, and robust data analysis strategies. Researchers currently employ a variety of techniques, including mass spectrometry, immunoassays, amino acid sequencing, and bioinformatics tools, to address the dive......

• • Sample Preparation Before Protein Mass Spectrometry

  Prior to conducting proteomics mass spectrometry, several preparatory steps must be taken to ensure both the successful execution of the experiment and the accuracy of the results. These steps include the selection of an appropriate mass spectrometer, meticulous sample preparation, protein extraction and purification, enzymatic digestion and labeling, sample preparation for mass spectrometry, and the precise calibration of the mass spectrometer.

• • Capillary Electrophoresis Chromatography Data Analysis

  Capillary Electrophoresis (CE) is a rapid, efficient, and high-resolution technique widely used for the separation and quantification of diverse biomolecules. To effectively interpret electropherogram data, it is essential to understand certain foundational methodologies and strategies. Data Preparation and Preprocessing: Initially, raw electropherogram data is exported from the experimental apparatus, comprising time and signal intensity at each detection point. The preprocessing phase involves noise......

• • How to Analyze the Results of Phosphorylated Protein WB

  Western blot (WB) analysis of phosphorylated proteins involves the following key steps: 1. Verification of Band Presence and Clarity: The first step is to examine the electrophoresis membrane for the presence of distinct protein bands and assess their clarity. For phosphorylated proteins, particular attention should be given to the presence of multiple bands, as phosphorylation can alter electrophoretic mobility.

• • Gene Knockout and Western Blot Validation Service

  Gene knockout represents a critical technique in biological research for elucidating gene functions. By eliminating specific genes, researchers can observe phenotypic changes in organisms, thus uncovering gene roles. Nevertheless, gene knockout remains a sophisticated and labor-intensive procedure necessitating advanced tools and methodologies. We provide specialized gene knockout services designed to facilitate the execution of these experiments with precision and efficiency.

• • Detection of the Immunogenicity of Type III Collagen

  Collagen is a crucial structural component of tissues, predominantly found in the skin, bones, tendons, blood vessels, and other connective tissues. Type III collagen belongs to the collagen family, and assessing its immunogenicity is essential for understanding its role in disease onset and progression.