Resources
Proteomics Databases
Metabolomics Databases
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Isotope labeling-based relative and absolute quantification (iTRAQ) is a technique that enables the relative and absolute quantification analysis of multiple samples through isotope labeling. The core of iTRAQ technology lies in using chemical labeling reagents to label proteins. These reagents react with the N-terminus of peptide chains and the side chain of lysine, creating a unique labeling pattern that allows the same peptide to be distinguished and quantified by mass spectrometry across different......
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• Dynamic Light Scattering Service
Dynamic light scattering service is a specialized analytical technique used for particle analysis, relying on dynamic light scattering (DLS) technology. This service is widely applied in the determination of particle size distribution and molecular dynamics studies, particularly for nanomaterials, biomolecules, and other particulate systems. The dynamic light scattering service has become an indispensable tool due to its simplicity, speed, and high sensitivity, especially when investigating nanopartic......
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Chemoproteomics is an interdisciplinary field that integrates chemical tools with proteomics techniques, aiming to provide a comprehensive analysis of protein structure, function, and their interactions with small molecules. The core principle of chemoproteomics involves using chemical probes or reactive molecules to specifically label proteins or their modification sites, which can then be detected using high-throughput techniques such as mass spectrometry. These labels enable the analysis of related......
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• Protein-Protein Interaction in Biological Processes
Protein-protein interaction (PPI) refers to the process by which two or more protein molecules form complexes through non-covalent binding. This interaction is fundamental to nearly all biological processes within living organisms. Proteins are the primary functional molecules in cells, performing critical roles such as enzyme catalysis, signal transduction, structural support, and material transport. Within each cell, a complex network of interactions coordinates diverse biological activities. For in......
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Amino acid test aims to accurately measure the content and composition of amino acids in biological samples. As the core constituents of proteins, amino acids play an essential role in biological processes. Consequently, their precise quantification is vital for both life science research and clinical diagnostics. Amino acid testing helps researchers gain a deeper understanding of protein structure, function, and metabolic pathways, and it has broad applications across various fields. Through accurate......
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Metabolomics and proteomics provide complementary insights into biological activities at the molecular level, with metabolomics focusing on small molecule metabolites and proteomics on proteins. These two fields are fundamental to modern biological research. Metabolomics studies metabolites, which are intermediate or final products of biochemical reactions. The dynamic fluctuations in metabolite levels reflect cellular metabolic states and responses to environmental changes. By analyzing the types, co......
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SWATH mass spectrometry is an advanced data-independent acquisition technique that has attracted considerable attention in proteomics research in recent years. This technology addresses the challenge of inadequate coverage of low-abundance proteins in traditional data-dependent acquisition approaches by fragmenting all ions within a predefined mass range. The introduction of this technique has significantly enhanced the coverage and reproducibility of quantitative proteomics data, as well as improved ......
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• Molecular and Cellular Proteomics
Molecular and Cellular Proteomics is a scientific discipline that investigates the composition, structure, function, and dynamic changes of proteins within living systems. Its goal is to uncover the mechanisms through which proteins function at the molecular and cellular levels. As a cutting-edge field in life sciences, it integrates mass spectrometry, advanced separation techniques, and bioinformatics, providing powerful tools for probing complex biological systems. By analyzing the expression profil......
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Bottom up proteomics is a widely used strategy for qualitative and quantitative analysis of proteins in proteomics research. Unlike top-down proteomics, the core principle of bottom up proteomics involves digesting the entire protein sample into smaller peptides prior to analysis. These peptides are then analyzed in detail by mass spectrometry (MS). This process allows for the precise investigation of protein structure, function, and dynamic changes, facilitating the efficient analysis of thousands of......
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Single-cell proteomics (SCP) is a cutting-edge analytical technique that deciphers protein composition and function at the resolution of individual cells. By uncovering cellular-level details, single-cell proteomics reveals intercellular heterogeneity and addresses limitations of traditional bulk proteomics, which provide only average or aggregate protein expression data. In plant research, single-cell proteomics circumvents challenges posed by rigid cell walls that hinder traditional methods from acc......
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