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Proteomics Databases
Metabolomics Databases
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HPLC protein quantification is a precise analytical method used to determine the concentration of proteins in samples utilizing the high resolution and sensitivity of HPLC systems. This technique is widely applicable across various fields, including biopharmaceuticals, food science, and environmental science. In biopharmaceuticals, it assists in drug development and quality control by ensuring protein content in pharmaceuticals complies with standards. In food science, it facilitates the detection of ......
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• C-Terminal Sequencing: 8 Critical Mistakes to Avoid in LC-MS/MS Analysis
LC-MS/MS analysis is extensively applied in proteomics for protein identification, post-translational modification profiling, and quantitative studies. However, during C-terminal sequencing, researchers often make avoidable mistakes in experimental design, data acquisition, and data analysis, which can compromise data quality and the reliability of conclusions. This article highlights eight critical mistakes commonly encountered in LC-MS/MS-based C-terminal sequencing, and provides practical strategie......
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• LC-MS/MS Based C-Terminal Sequencing: Technical Advances and Challenges
One of the central objectives of proteomics is to elucidate the complete amino acid sequences of proteins. In this context, C-terminal sequencing has attracted increasing attention in recent years due to its critical role in understanding protein modifications, degradation pathways, and biological functions. The rapid advancement of liquid chromatography–tandem mass spectrometry (LC-MS/MS) has offered powerful analytical capabilities for C-terminal sequencing. However, challenges remain due to the lim......
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• How to Improve C-Terminal Sequencing Sensitivity and Accuracy with LC-MS/MS?
In C-terminal sequencing with LC-MS/MS, improving sensitivity and accuracy requires a systematic and integrated approach involving sample preparation, optimization of instrumental settings, and data analysis strategies. The following are major strategies for enhancement: Selection of Appropriate Proteases and Sample Preparation Strategies The key to successful C-terminal sequencing lies in achieving high specificity and efficiency in generating C-terminal peptides. While conventional proteases such ......
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BiFC analysis is a technique used to investigate protein-protein interactions. This method leverages the reconstitution of fluorescent protein fragments, where a fluorescent protein (e.g., yellow fluorescent protein (YFP) or enhanced green fluorescent protein (EGFP)) is divided into two non-fluorescent fragments, each fused to a different protein of interest. When these proteins interact within cells or in vitro, the protein fragments come into proximity and reassemble, restoring the fluorescence sign......
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• Antibody Screening and Identification
Antibody screening and identification are critical steps in life sciences research and the development of biopharmaceuticals. Antibodies play a vital role in the immune system's ability to recognize and neutralize pathogens, and therefore have broad applications in disease diagnosis, treatment, and research. This process involves selecting antibodies from a pool of candidates that possess specific target-binding capabilities and the required characteristics. Typically, this process encompasses the con......
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The human protein interactome is a vital area of contemporary life sciences research, representing the complex network formed by protein interactions both inside and outside of cells. As essential molecular participants in nearly all biological processes-such as signal transduction, metabolic regulation, cell cycle control, and immune response-proteins typically do not function in isolation. Instead, they form intricate networks through interactions, enabling specific biological functions. Therefore, ......
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• End-Group Analysis of Peptides
End-group analysis of peptides is a technique in proteomics research used to study and identify the start and end positions of amino acid sequences in proteins or peptide chains. This analysis not only aids scientists in understanding protein structure and function but also reveals the metabolic pathways and mechanisms of action within organisms. The peptide end groups-typically referring to the N-terminus (amino terminus) and the C-terminus (carboxyl terminus)-are critical for protein bioactivity and......
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• End-Group Analysis of Protein
End-group analysis of protein is a technique in proteomics research primarily used to determine the amino acid sequences at both termini of protein molecules. Typically, protein ends include the N-terminus (amino terminus) and the C-terminus (carboxyl terminus). This analysis not only provides structural information but also elucidates various biological functions. For example, the N-terminus is closely linked to signal peptide recognition, protein localization, and cellular half-life, while the C-ter......
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• Detecting Phosphorylated Proteins
Detecting phosphorylated proteins refers to the process of identifying and analyzing the presence and localization of phosphate groups on protein molecules using specific experimental techniques and methods. Phosphorylation, the most common type of post-translational modification (PTM) in proteins, regulates protein activity, localization, and interactions by adding phosphate groups to serine, threonine, or tyrosine residues. It plays a critical role in biological processes such as cell signaling, met......
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