Resources
Proteomics Databases
Metabolomics Databases
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• Proteomics Mass Spectrometry
Proteomics mass spectrometry is a fundamental technique in proteomics research, instrumental in identifying and quantifying proteins within biological samples. This approach provides insights into the intricate protein networks within organisms. With the advancement of genomics, genetic sequence data alone are insufficient to capture the organism's complexity and dynamic characteristics. Mass spectrometry in proteomics allows for comprehensive analysis of proteins, facilitating the identification, qua......
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Quantifying western blots aims to reveal biological processes by quantitatively analyzing protein expression levels. This technique builds on traditional western blotting but incorporates quantitative analysis, allowing researchers to measure target protein expression with greater accuracy. Quantifying western blots is essentially a quantitative analysis method within western blotting. Similar to Southern blot and Northern blot hybridization techniques, western blotting uses polyacrylamide gel electro......
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Protein profile refers to the analysis and measurement of the types, quantities, and variations of proteins within a biological system to comprehensively understand their functions and dynamic changes in different biological processes. The application of protein profiling is extensive. During disease onset and progression, the protein profile of biological samples undergoes characteristic changes. For example, in certain cancer patients, the expression levels of specific proteins in serum may increase......
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• Can LC-MS Detect Enzymatic Glycosylation Modifications
Liquid chromatography-mass spectrometry (LC-MS) is a powerful tool for detecting enzymatic glycosylation modifications. Glycosylation, a prevalent post-translational modification (PTM) of proteins, is crucial in various biological processes such as cellular signaling, protein folding, and immune recognition. LC-MS is especially advantageous in glycosylation analysis because it provides comprehensive data on glycosylation sites, including the glycosylation type and glycan chain structure.
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• Peptide Sequencing for Protein Analysis
Peptide sequencing is a biotechnological method used to elucidate the structural and functional relationships of proteins through analyzing the amino acid sequences in peptide chains. As peptides are fundamental constituents of proteins, understanding their sequences allows researchers to investigate their roles in biological functions more thoroughly. The peptide sequencing process typically involves several critical steps: sample preparation, enzymatic digestion, separation and detection, and data a......
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• Glycan Mass Spectrometry Analysis
In biological sciences, mass spectrometry is widely employed for the qualitative and quantitative analysis of complex samples, such as proteins and nucleic acids. Glycan mass spectrometry refers to the application of mass spectrometry for the characterization of glycans. Glycans are biological macromolecules that are abundantly present in living organisms and play crucial roles in various biological processes, including cell recognition, signal transduction, and immune response.
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• Number of Disulfide Bonds in a Protein
Disulfide bonds play a crucial role in protein structure by stabilizing the three-dimensional conformation, which is essential for proper protein function. These bonds are covalent linkages formed between the sulfur atoms of two cysteine residues. Therefore, to estimate the number of disulfide bonds in a protein, it is necessary to identify all cysteine residues and assess their potential pairings.
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• Overview of Core Technologies for Protein Identification
Advancements in technology have transformed protein identification from basic qualitative analysis into a comprehensive system integrating multidisciplinary approaches. The primary objective is to characterize protein species, structures, and post-translational modifications (PTMs), facilitating research in life sciences, disease diagnostics, and drug development. The current technological framework for protein identification encompasses mass spectrometry, immunodetection, structural analysis techniqu......
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ICAT mass spectrometry is a powerful proteomics technique for quantifying relative protein abundance. In biomedical research, ICAT enables precise protein quantification, offering valuable data for early disease diagnosis and treatment. In drug development, this method facilitates the rapid screening and identification of drug targets, enhancing the efficiency of novel therapeutics. Additionally, in environmental monitoring, ICAT mass spectrometry allows for the precise detection of trace compounds in......
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Amino acid quantitation is a widely employed technique for analyzing and measuring amino acid content in various samples. As the fundamental building blocks of proteins, amino acids play essential roles in numerous metabolic pathways. Beyond their function as protein constituents, amino acids contribute to biomolecule synthesis, regulate biochemical reactions, and maintain osmotic balance in biological systems. Thus, precise quantitation of amino acid composition is critical for understanding physiolo......
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