Resources

Proteomics Databases



Metabolomics Databases

• • Application of Mass Spectrometry in Protein Oxidative Modification Analysis

  Protein oxidation modifications (POMs) are prevalent molecular processes within living organisms, playing critical roles in cellular function, signal transduction, and the development of diseases. As mass spectrometry (MS) technology has advanced, it has become a pivotal tool in analyzing and identifying these modifications. The high sensitivity and resolution of MS enable the precise detection and quantification of protein oxidation modifications within complex biological samples.

• • Workflow of Protein Oxidative Modification Analysis Using Nano-LC-MS/MS

  Protein oxidation modification is a common post-translational modification associated with cellular oxidative stress. Analyzing these modifications is crucial for understanding cellular signaling pathways, metabolic regulation, and the pathophysiology of various diseases. Nano-LC-MS/MS (Nano-Liquid Chromatography coupled with Tandem Mass Spectrometry) is widely regarded as the optimal technique for analyzing protein oxidation modifications due to its superior sensitivity and resolution.

• • Principle of Protein Oxidative Modification Analysis Based on Mass Spectrometry

  Protein oxidation modification is a prevalent biochemical phenomenon that involves oxidative alterations of amino acid residues in proteins. These modifications are crucial in various biological processes, such as cellular signaling, metabolic regulation, and aging. Due to its high sensitivity and resolution, mass spectrometry (MS) has become the primary method for analyzing protein oxidation modifications.

• • Mechanism of Protein Sumoylation in Subcellular Localization

  Protein SUMOylation is a critical post-translational modification involving the covalent attachment of SUMO (Small Ubiquitin-like Modifier) to target proteins. Unlike ubiquitination, SUMOylation does not mark proteins for degradation but instead regulates their function by altering their activity, interaction partners, or subcellular localization. SUMOylated proteins are often found enriched in specific subcellular compartments, such as the nucleus, cytoplasm, or cytoskeleton.

• • Application of Protein Sumoylation Identification in Cellular Processes

  Protein SUMOylation refers to the covalent attachment of Small Ubiquitin-like Modifier (SUMO) proteins to target proteins through enzymatic reactions. This post-translational modification plays a pivotal role in regulating protein function, localization, and stability. In recent years, significant attention has been directed towards the role of SUMOylation in various cellular activities, particularly in transcriptional regulation, DNA repair, and cell cycle control.

• • Principle of Protein Sumoylation Identification

  SUMOylation, involving the covalent attachment of Small Ubiquitin-like Modifier (SUMO) to target proteins, is a critical post-translational modification that regulates diverse cellular processes, including nuclear functions, signal transduction, and transcriptional regulation. Accurate detection of SUMOylation sites and the proteins modified by SUMO is essential for understanding the functional roles of this modification.

• • Workflow of Protein Sumoylation Identification

  Protein SUMOylation (Small Ubiquitin-like Modifier, SUMO) is a critical post-translational modification (PTM) that regulates various cellular processes, including protein function, signaling pathways, and gene expression. Understanding the biological significance of SUMOylation requires precise identification and analysis of SUMOylated proteins.

• • Protein Gel Band Identification: From Cross-Linking to Interaction Analysis

  Protein gel strip identification is a commonly used experimental method that helps researchers determine the interaction relationships between proteins.

• • Circular Dichroism Analysis of Peptide Drugs

  Peptide drugs refer to a category of drugs that use peptides for the treatment of diseases. As one of the hotspots in drug development, peptide drugs have significant efficacy, strong specificity, and low toxicity. They are widely used in the treatment of various diseases, including metabolic diseases, neurological diseases, immune diseases, and tumors.

• • Purity Analysis of Antibody Drugs (CE-SDS, SDS-PAGE, SEC, RP, etc.)

  Antibody drugs are a class of drugs that treat diseases through artificially synthesized antibodies. They achieve therapeutic purposes by specifically binding to target molecules. Common types of antibody drugs include monoclonal antibodies, artificially synthesized antibody fragments, immunotoxins, antibody-drug conjugates, etc.