Resources

Proteomics Databases



Metabolomics Databases

• • Phosphoproteome Sequencing of Plants

  Phosphoproteomics sequencing in plants is an important method to study the molecular mechanisms of plant responses to environmental changes by analyzing the phosphorylation modifications of plant proteins. Through phosphoproteomics sequencing, the degree of protein phosphorylation under specific conditions can be quantitatively analyzed, thereby investigating its function and regulatory mechanisms.

• • Can Mass Spectrometry Detect Types of Protein Modifications

  Mass spectrometry (MS) is a powerful technique for detecting and quantifying proteins and their post-translational modifications in biological samples. In mass spectra, modified peptides exhibit distinct mass-to-charge ratios compared to their unmodified counterparts. Analyzing these differences allows for the identification of modification sites and enables the inference of both the type and extent of modifications.

• • Identification of Protein Oxidative Modifications

  Oxidative modification represents a prevalent form of post-translational protein modification, significantly contributing to key physiological and pathological processes, including cell signaling, protein degradation, aging, and disease progression. Comprehensive identification and quantitative analysis of these modifications are pivotal for elucidating their roles in these mechanisms. However, the identification process is inherently complex, necessitating the integration of advanced techniques such as....

• • Proteomics Mass Spectrometry Identification of Malonylated Proteins

  Malonylation is a significant post-translational modification of proteins, playing regulatory roles in various biological processes such as gene expression, cell cycle, and apoptosis. Mass spectrometry-based proteome-wide analysis of protein malonylation is an effective approach for detecting and analyzing malonylated proteins, facilitating a deeper understanding of malonylation's biological functions.

• • Types and Applications of Modified Proteomics

  Protein modification proteomics, an emerging field derived from proteomics, focuses on the changes proteins undergo post-translation through the addition or removal of chemical groups. This field encompasses various types of modifications, including phosphorylation, acetylation, ubiquitination, methylation, and glycosylation. These modifications have significant implications in fields such as life sciences, medical research, and drug development.

• • What Antibody Is Used to Detect Acetylation

  Acetylation is a widely occurring protein modification in organisms, playing an important regulatory role in protein function and localization. In organisms, acetylation mainly manifests as N-terminal acetylation and lysine acetylation. Detection of acetylation is primarily achieved through antibodies, and the selection of acetylation antibodies mainly includes site-specific antibodies corresponding to acetylation sites and pan-acetylation antibodies.

• • Phosphorylation Mass Spectrometry of Cell Samples

  Mass spectrometry is a technique for identifying chemical compounds by determining the mass-to-charge ratio of ions. In biological research, it is frequently employed to investigate biochemical processes, including protein phosphorylation. The workflow for phosphorylation mass spectrometry of cell samples comprises four key steps: preparation of cell samples, protein separation and detection, phosphorylation-specific mass spectrometry analysis, and subsequent data interpretation.

• • Which Antibody Is Used for Immunoprecipitation in Protein Ubiquitination

  Anti-ubiquitin antibodies serve as vital tools for investigating protein ubiquitination. They are particularly valuable in immunoprecipitation assays for the enrichment and detection of ubiquitinated proteins. This paper presents a detailed immunoprecipitation protocol using anti-ubiquitin antibodies aimed at exploring protein ubiquitination processes, essential for understanding mechanisms such as protein degradation and signal transduction.

• • Antibody Sequencing and Immunome Sequencing

  Antibody sequencing is a technique that analyzes the amino acid sequences of antibodies (immunoglobulins), which helps researchers understand the specificity of antibody-antigen interactions. Immunosequencing, on the other hand, examines DNA sequences associated with the immune system, aiding researchers in understanding immune responses to various diseases and infections. These insights enable the design and development of more effective vaccines and antibody therapies.

• • iTRAQ and TMT Label-Based Quantitative Proteomics Utilize Mass Spectrometry Information for Quantitative Analysis

  In proteomics research, iTRAQ (Isobaric Tag for Relative and Absolute Quantitation) and TMT (Tandem Mass Tags) are widely used for quantitative analysis. These methods involve labeling proteins or peptides with tags of identical mass, allowing quantification through the intensity of reporter ions during mass spectrometry.