Resources
Proteomics Databases
Metabolomics Databases
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• Immunoprecipitation and Coimmunoprecipitation
Immunoprecipitation and coimmunoprecipitation are fundamental techniques in proteomics, widely applied to investigate protein structures, functions, and dynamic changes in cellular environments. These methods utilize the specific binding between antibodies and target proteins. Immunoprecipitation (IP) isolates a target protein from complex biological samples via antibodies, enabling analysis of protein purity and interaction with other proteins through coimmunoprecipitation. The Co-IP technique extend......
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• Co Immunoprecipitation Assay
The co immunoprecipitation assay serves as a cornerstone technique in protein interaction studies, employing specific antibodies to isolate protein complexes from complex biological samples. This method capitalizes on the selective binding of antibodies to target proteins, facilitating the precipitation of associated protein complexes and elucidating intracellular protein interaction networks. Its versatility extends to identifying protein partners, unraveling biological mechanisms, understanding dise......
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• ABPP Activity Based Protein Profiling
ABPP activity based protein profiling is a function-oriented proteomics approach that prioritizes the activity and functional states of proteins over traditional metrics such as expression levels or sequence characteristics. This technique has emerged as a pivotal method for understanding protein functionality, identifying active proteins, and elucidating protein-small molecule interactions. By employing specific reactive probes, ABPP selectively labels active proteins, enabling their capture, enrichm......
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• HPLC Tandem Mass Spectrometry
High-performance liquid chromatography tandem mass spectrometry (HPLC tandem mass spectrometry, HPLC-MS/MS) is a critical analytical technique widely applied in modern analytical chemistry, particularly for analyzing components in complex biological samples. By combining the separation power of high-performance liquid chromatography (HPLC) with the analytical capabilities of mass spectrometry (MS), HPLC-MS/MS enables the efficient separation, identification, and quantification of samples within a sing......
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Protein quantification analysis is one of the core tasks in proteomics research, aiming to precisely quantify protein levels by measuring the relative or absolute abundance of proteins in a sample. This process not only unveils the dynamic changes in cellular protein levels but also provides valuable insights into various biological conditions, such as organism health, disease states, and drug responses. Proteins are fundamental functional molecules in living organisms and participate in almost every ......
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Tandem mass spectrometers enable precise qualitative and quantitative analysis of complex mixtures by connecting two or more mass analyzers in series, leveraging their ability to separate, detect, and analyze ions. These instruments are especially effective in analyzing complex, low-abundance biomolecules such as proteins, metabolites, and drug molecules. Their application in proteomics is particularly significant. By conducting precise mass spectrometry analysis of peptides, scientists can decode the......
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Tandem mass spectrometry is a highly sensitive analytical technique for investigating the components of complex mixtures, with significant applications in proteomics research. This method divides the mass spectrometry process into sequential steps, enabling the identification and quantification of specific molecules in complex samples. The basic principle involves selecting a target ion in the first mass spectrometer and fragmenting it using collision-induced dissociation (CID) or other activation met......
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The peptide mapping workflow encompasses enzymatic digestion, chromatographic separation, and mass spectrometry analysis of target proteins or antibodies to construct detailed peptide maps. These maps provide insights into molecular structures, post-translational modifications, and sequence variants. As a cornerstone for protein structure characterization and quality control, this workflow plays an integral role in drug development, biomarker discovery, and process optimization. Basic Workflow The p......
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Peptide Mass Mapping is a core data form in proteomics and bioanalytical research. It is generated by detecting the mass-to-charge ratio (m/z) of peptides using a mass spectrometer, producing specific spectral signals to deduce peptide sequence information and post-translational modifications. It serves as a crucial tool for understanding protein molecular structure, function, and interactions, holding irreplaceable value in biopharmaceutical development, disease mechanism research, and fundamental li......
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• Unlocking Hidden Secrets: Comprehensive Strategies for Unknown Protein Analysis
The field of unknown protein analysis relies on systematic approaches to uncover the structure, function, and biological roles of these enigmatic molecules. Advancements in Genomics and Transcriptomics The foundation of unknown protein analysis lies in leveraging high-throughput sequencing to extract genomic and transcriptomic data. These datasets enable the identification of novel genes and transcripts encoding unknown proteins. For instance, whole-genome sequencing may reveal genes with no known f......
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