Resources

• • Glycoprotein Identification Unveils Structure-Function Relationship of Glycosylated Proteins

  Glycoproteins are a class of proteins that undergo glycosylation modification, playing important structural and functional roles in organisms. Studying the relationship between the structure and function of glycoproteins has significant implications for understanding cellular signal transduction, the immune system, and disease development.

• • Proteomics Analysis Revealing Protein Composition and Function in Biological Systems

  Proteins are one of the most important molecules in biological systems, playing various crucial roles within cells. Understanding the composition and functions of proteins is essential for studying biological processes, developing drugs, and diagnosing diseases. However, the complexity and diversity of proteins make their study challenging. In order to address these issues, proteomic analysis has emerged.

• • Detection of Membrane Proteins with High-Resolution Mass Spectrometry

  Membrane proteins are vital components of the cellular membrane, playing essential roles in substance transport, signal transduction, and intercellular communication. Given their biological significance, the study of membrane proteins has become a focus in life sciences. However, due to their low solubility and complex structural features, the isolation and analysis of membrane proteins present substantial challenges.

• • Functional Analysis of Membrane Proteins Using Orbitrap LC-MS

  Membrane proteins perform various critical biological functions, such as signal transduction, substance transport, and intercellular communication. However, due to their hydrophobic nature and complex structures, functional analysis of membrane proteins has long been a challenge in biological research.

• • Proteomics Workflow in Paraffin-Embedded Tissue Samples

  Formalin-Fixed Paraffin-Embedded (FFPE) tissue samples are frequently used in clinical pathology for long-term preservation of biological specimens. The formalin fixation process, while preserving tissue structure, induces protein cross-linking, presenting challenges for downstream proteomics analysis. However, with carefully optimized workflows, researchers can successfully perform proteomic studies on FFPE tissues.

• • Proteomics Analysis of Exosome Samples Based on LC-MS/MS

  Exosomes are nanoscale vesicles secreted by cells, containing proteins, lipids, nucleic acids, and other biological molecules. They play a crucial role in intercellular communication, immune regulation, tumor metastasis, and various physiological and pathological processes, making them a hotspot in biomedical research.

• • Quantitative Analysis of Proteomics in Subcellular Fractions

  Quantitative analysis of proteomics in subcellular fractions is a critical technique for elucidating the distribution and functional activities of proteins in different regions of a cell. Cells are complex structures, with each subcellular component, such as the nucleus, mitochondria, endoplasmic reticulum, and plasma membrane, exhibiting distinct forms and functions.

• • Structural Characterization of N-Glycan Chains Using Permethylation and Mass Spectrometry

  N-glycan chains, as part of protein post-translational modifications (PTMs), play crucial roles in cell recognition, signal transduction, and immune responses. Understanding the structure of N-glycans is vital for uncovering their biological functions. However, due to the complexity of N-glycans, structural characterization has been a significant challenge in glycomics research.

• • Mechanism of Peptide Structure Determination

  Peptide structure determination is fundamental to understanding protein functions and biological processes. An accurate determination of peptide structures is essential for studying protein functions, molecular interactions, and their role in diseases. The process involves determining the peptide’s primary structure, resolving spatial conformation, identifying molecular interactions, and characterizing post-translational modifications (PTMs).

• • Workflow of Peptide Structure Determination

  Determining peptide structure is essential for elucidating the amino acid sequence and spatial conformation, which is critical for biological research and drug development. This process includes several steps, from sample preparation to final data analysis, with each step impacting the experiment's accuracy and success.