Resources

• • Workflow of Peptide Biomarker Discovery and Validation

  Peptide biomarkers are small molecular fragments derived from the breakdown of proteins and play a crucial role in disease diagnosis, therapeutic monitoring, and drug development. With the rapid advancements in proteomics, the discovery and validation of peptide biomarkers have become a critical field in life sciences research.

• • Application of Mass Spectrometry-Based Peptide Identification

  Mass spectrometry (MS), a high-sensitivity, high-specificity, and high-throughput analytical technique, has become a powerful tool in proteomics. In particular, MS is widely used for peptide identification, providing qualitative and quantitative analysis of peptides generated from protein digestion.

• • Workflow of Mass Spectrometry-Based Peptide Identification

  Mass spectrometry (MS) is a widely used analytical tool in proteomics research, especially for peptide identification. Through MS, protein samples can be deeply analyzed to reveal expression levels, modification states, and protein-protein interactions. The workflow for peptide identification using mass spectrometry involves a series of steps, ranging from sample preparation to data analysis.

• • Advantages and Disadvantages of Mass Spectrometry-Based Peptide Identification

  Mass spectrometry (MS) is a highly sensitive and specific analytical technique widely used in proteomics research, especially in peptide identification. Peptide identification involves analyzing the enzymatic digests of protein samples to determine their amino acid sequences and structures. MS has become one of the dominant methods for peptide identification, offering numerous unique advantages while also facing certain limitations.

• • Principle of Mass Spectrometry-Based Peptide Identification

  Mass spectrometry (MS) is a powerful analytical tool widely used in proteomics research. Through MS, researchers can conduct in-depth analysis of complex protein mixtures, achieving significant advancements in peptide identification. MS offers high sensitivity and throughput, making it a robust method for studying complex biological systems.

• • Testing of Raw Materials for Recombinant Protein Vaccines

  Recombinant protein vaccines are a type of vaccine that does not contain the complete pathogen but is formulated with specific protein antigens produced in heterologous expression systems. Common heterologous expression systems include bacteria, mammalian cells, plant cells, and insect cells, and the appropriate system is usually selected based on the antigen being produced.

• • Quantification of Free Radicals in Antibody Drugs

  Antibody drugs are a class of protein drugs that target specific targets (cell surface proteins) within the body. They are essentially antibodies with a single determinate specificity produced by a single B lymphocyte clone or its derivative. Antibody drugs are mainly produced through artificial synthesis or biotechnology, and these drugs can specifically recognize and bind to specific targets (usually proteins) within the body to achieve therapeutic effects.

• • Recombinant Protein Drug and Receptor Affinity Detection

  Recombinant protein drugs are protein-based therapeutic drugs produced using DNA recombinant technology or other biotechnological methods. They include cytokines, peptide hormones, recombinant enzymes, monoclonal antibodies, fusion proteins, and so on. Compared to traditional small molecule synthetic drugs, recombinant protein drugs have advantages such as high specificity, low toxicity, and significant therapeutic effects.

• • Regulatory Mechanisms of Protein Glycosylation Modifications

  Glycosylation modification of proteins plays a critical role in cellular functions and signal transduction, serving as an important biochemical modification. Glycosylation modification can affect the stability, activity, localization, and interactions of proteins, thereby regulating various biological processes.

• • Glycosylation Analysis Reveals Diversity and Function of Protein Modifications

  Protein glycosylation modification, as an important biochemical process, plays a crucial role in cellular function and signal transduction. Glycosylation modification can not only alter the biological activity and stability of proteins but also participate in regulating important biological processes such as protein-protein interactions, recognition, and mediation of cell-cell interactions.