Resources

• • Analytical Methods for Proteomic Modifications and Key Techniques for Protein Modification States Detection

  Protein modification research is one of the important research directions in the field of biopharmaceuticals. Understanding the analytical methods of protein modifications is of great significance for revealing protein function and disease mechanisms.

• • Mechanism of Peptide Analysis

  Peptide analysis has become a pivotal tool in biochemical and molecular biology research, serving as a cornerstone in identifying, quantifying, and characterizing peptide molecules from complex biological samples. This process leverages advanced technologies such as mass spectrometry (MS), high-performance liquid chromatography (HPLC), and bioinformatics tools.

• • Application of Peptide Analysis

  Peptide analysis is a technique used to identify and quantify peptides in biological samples, with widespread applications in various research fields. With the continuous advancements in mass spectrometry and liquid chromatography technologies, the accuracy and sensitivity of peptide analysis have significantly improved, providing crucial technical support for basic research, clinical medicine, and biotechnology development. Below are the major application areas of peptide analysis.

• • Workflow of Peptide Analysis

  Peptide analysis involves studying peptides to reveal the function, structure, and interactions of proteins. Since proteins are vital participants in biological processes, peptide analysis has become an indispensable tool in modern biological research.

• • Principle of Peptide Analysis

  Peptides are short chains of amino acids linked by peptide bonds, typically consisting of 2 to 50 amino acid residues. As subunits of proteins, peptides play crucial roles in biological processes, such as signal transduction, immune response, and metabolic regulation. Therefore, studying peptides is of great importance for understanding protein functions, disease mechanisms, and drug development.

• • Mechanism of Peptidomics Analysis

  Peptidomics analysis is a crucial technique that investigates the composition, structure, and function of specific peptides within protein molecules, thereby revealing the functions and regulatory mechanisms of proteins. Peptides are the building blocks of proteins, and advanced techniques such as mass spectrometry can break down proteins into multiple peptides for further analysis, enabling a comprehensive understanding of the proteins.

• • Application of Peptidomics Analysis

  Peptidomics, the comprehensive analysis of peptides in biological samples using mass spectrometry, plays a crucial role in modern biological research. Peptides are degradation products of proteins and reflect the functional state of proteins within cells. Therefore, peptidomics analysis not only serves as an effective complement to proteomics research but also holds unique value in various biological research fields. Below are the main applications of peptidomics analysis in different domains:

• • Workflow of Peptidomics Analysis

  Proteomics analysis is a systematic approach to studying complex protein mixtures through mass spectrometry (MS). This technique breaks down proteins into smaller peptides for analysis, providing a comprehensive view of the proteome. The workflow of proteomics analysis typically consists of the following steps:

• • Principle of Peptidomics Analysis

  Peptidomics is a significant branch of proteomics, focusing on the study of peptides generated after protein digestion under specific conditions. By detecting and identifying peptides, peptidomics can reveal the structure, function, and post-translational modifications of proteins, thereby assisting researchers in better understanding biological processes within cells.

• • Analysis of N-Glycosylation Sites in Proteins Using PNGase F

  N-glycosylation is a common post-translational modification where sugar chains (glycans) are attached to specific amino acid residues of proteins, altering their function, stability, and structure. N-glycosylation is widespread in cell surface and secreted proteins, playing critical roles in intercellular communication, immune responses, and other biological processes.