Resources

• • Quantitative Analysis of N-Glycosylation Sites Based on Isotopic Labeling Mass Spectrometry

  N-glycosylation is a widely occurring form of post-translational modification, playing a vital role in protein folding, quality control, stability, trafficking, and immune responses, thereby impacting various cellular processes. The quantitative analysis of N-glycosylation sites provides insights into the modification levels of proteins, contributing to the understanding of their specific functions in biological mechanisms.

• • Analysis of O-Glycans via Chemical Hydrolysis

  O-glycans are an essential form of glycosylation in glycoproteins, playing crucial roles in regulating cellular communication and immune recognition. Studying the structure and function of O-glycans provides insights into these biological processes. Chemical hydrolysis is a commonly used technique to release and analyze O-glycans from glycoproteins.

• • Analysis of N-Glycan Profiles Using MALDI-TOF-MS

  N-glycans are a crucial modification of glycoproteins on the cell surface, involved in various biological processes such as protein folding, cell-cell communication, and immune response. Accurate analysis of N-glycan structures and compositions is essential for understanding their biological and medical roles. In recent years, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) has become a powerful tool for studying N-glycan profiles

• • Detection of O-Glycans by MALDI-TOF-MS

  O-glycans, a common type of glycosylation modification, are widely present on the surface of proteins and play critical roles in various biological processes such as cell recognition, signal transduction, and immune responses. Due to the structural complexity and high heterogeneity of O-glycans, mass spectrometry-based methods have become indispensable tools for analyzing glycoproteins and studying glycosylation patterns.

• • Detection of Glycosylation Sites and Glycoforms Based on LC-MS/MS

  Glycosylation is a crucial post-translational modification that influences protein folding, stability, and function. Accurate detection of glycosylation sites and glycoforms is essential for studying biological processes, disease mechanisms, and drug development. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) has become the primary method for detecting glycosylation sites and glycoforms due to its high sensitivity and resolution.

• • Mechanism of Proximity Extension Assay in Olink Analysis

  Olink analysis is a highly sensitive protein detection platform with broad applications in biomarker discovery, clinical research, and drug development. One of its core technologies is the Proximity Extension Assay (PEA), which utilizes antibody recognition combined with DNA extension to detect hundreds of proteins in complex biological samples simultaneously.

• • Application of Olink Proteomics in Biomarker Discovery

  Olink proteomics technology, known for its highly sensitive and precise protein quantification capabilities, has become a pivotal tool in the field of biomarker discovery. By combining multiplex immunoassays with high-throughput liquid chip technology, Olink allows the quantification of a large number of proteins in a single experiment. This not only shortens research timelines but also improves the accuracy and reproducibility of results.

• • Advantages and Disadvantages of Olink Analysis

  Olink analysis is a cutting-edge proteomics tool based on Proximity Extension Assay (PEA) technology, increasingly applied in biomedical research. Its combination of high sensitivity, specificity, and high-throughput detection allows the measurement of hundreds of proteins from minimal sample volumes. Below, we explore in detail the advantages and disadvantages of this technology.

• • Workflow of Olink Proteomics

  Olink proteomics is a high-throughput technique leveraging protein-specific antibodies, widely used in life sciences for the simultaneous detection and quantification of numerous proteins. This method uses a unique immuno-amplification mechanism, enabling the analysis of hundreds to thousands of target proteins in a single assay, which is especially useful in clinical and basic research.

• • Principle of Olink Proteomics

  Olink proteomics is an advanced technology based on Proximity Extension Assay (PEA), allowing the simultaneous measurement of multiple target proteins with exceptional sensitivity and specificity. The core concept of PEA involves the use of antibodies that specifically bind to target proteins, coupled with precise protein quantification using DNA sequence tags attached to the antibodies.