Resources

• • Detection of N- and O-Glycosylation Sites Based on LC-MS/MS

  Glycosylation is an essential modification of biomolecules, particularly proteins, significantly impacting their structure and function. N-glycosylation and O-glycosylation are the two primary forms of glycosylation, involving nitrogen and oxygen atoms of amino acid residues, respectively.

• • Quantitative and Qualitative Glycoprotein Analysis via Mass Spectrometry

  Glycoproteins are important biomolecules widely found in living organisms, participating in various biological processes such as cell recognition, signal transduction, and immune response. The complexity of glycoprotein structure and function makes it a significant area of biological research. Recent advancements in mass spectrometry (MS) technology have provided a powerful tool for the quantitative and qualitative analysis of glycoproteins.

• • Steps of N-glycan Profiling Using Enzyme Digestion and MALDI-TOF-MS

  N-glycomics research is a crucial field for exploring the role of N-glycosylation in the functions and interactions of molecules within biological systems. N-glycosylation plays a key role in various biological processes, and its abnormalities are often associated with diseases. The use of enzymatic digestion and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF-MS) allows for efficient analysis of the structure and composition of N-glycans.

• • N-glycan Profiling Based on MALDI-TOF-MS Detection

  N-glycosylation is an important post-translational modification process in biological systems, significantly affecting protein function and stability. N-glycomics analysis is a vital tool for studying N-glycosylation, and the method based on MALDI-TOF-MS (Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry) has gained widespread attention for its high throughput and sensitivity.

• • Quantitative Glycomic Analysis Based on HILIC-UHPLC

  Glycomics is the study of carbohydrate biomolecules, particularly their synthesis, metabolism, and functions in living organisms. With the growing understanding of the role of carbohydrates in cell function and diseases, quantitative analysis in glycomics has become increasingly important. HILIC-UHPLC (Hydrophilic Interaction Liquid Chromatography-Ultra High Performance Liquid Chromatography) is widely used in quantitative glycomics analysis due to its high separation efficiency and sensitivity.

• • Detection of S-Nitrosylation Using Antibody Enrichment and MS

  S-nitrosylation is an important post-translational modification involving the addition of a nitric oxide (NO) moiety to the thiol group of cysteine residues. This modification plays a crucial role in cellular signaling, redox reactions, and various physiological processes.

• • Analysis of Multi-Pathway Phosphoproteomics Using Orbitrap Fusion Lumos

  Phosphorylation, an important post-translational modification, is widely involved in various biological processes such as cell signaling, metabolic regulation, and cell cycle control. It introduces phosphate groups to tyrosine, serine, and threonine residues through enzymatic reactions, regulating protein activity, stability, and interactions.

• • Analysis of Quantitative Proteomics Using MRM

  Quantitative proteomics is a vital tool for understanding biological processes, especially in biomarker discovery, disease mechanism research, and drug development. Multiple Reaction Monitoring (MRM) is a highly sensitive mass spectrometry technique widely applied in protein quantification analysis. The advantage of MRM lies in its ability to accurately measure the abundance of target proteins, providing essential information about their biological functions.

• • Quantitative Proteomics Detection Using SWATH

  Quantitative proteomics is a powerful tool for analyzing protein expression levels in cells or biological samples. With technological advancements, the emergence of SWATH (Sequential Windowed Acquisition of All Theoretical Mass Spectra) has provided new insights into quantitative proteomics. SWATH combines the advantages of high-resolution mass spectrometry and high-throughput detection, allowing researchers to obtain comprehensive and accurate protein quantification information in complex samples.

• • Quantitative Proteomics Workflow Based on Label-Free Methods

  Quantitative proteomics serves as a vital tool for investigating protein abundance and variability in cells or tissues. Recently, label-free methods have emerged as a mainstream technology in quantitative proteomics due to their ease of use and high throughput.