Post-Translational Modifications Proteomics Service
Post-translational modifications (PTMs) are covalent and usually enzymatic modifications on proteins. Common PTMs include phosphorylation, acetylation, ubiquitination, glycosylation, and so on. These PTMs can cause huge influences on protein structure, distribution, and function, hence, increasing the complexity of proteome to a greater extent. Protein PTMs is generally analyzed by measuring the mass increased on the modified peptides. Since PTMs are generally present in very low abundance, specific enrichment procedures are required before PTMs identification. MtoZ Biolabs has established a powerful and professional Post-Translational Modifications Proteomics Service, which includes Thermo Fisher Q ExactiveHF and Obitrap Fusion Lumos mass analyzer system, coupled with Nano-LC system. Our aim is to provide the most professional support for our clients’ research.
Analysis Workflow
PTMs Analysis Service Categories
MtoZ Biolabs provides protein phosphorylation analysis service with highly efficient phospho-peptides enrichment processing and Nano LC-MS/MS analysis. Coupled with SILAC/iTRAQ labeling, this service can be applied to large-scale phospho-proteomics analysis.
MtoZ Biolabs provides precise acetyl-proteomics analysis service using Nano LC-MS/MS. We also use CST acetylation-specific antibodies for acetyl-peptide enrichment, and 2-3 different enzymes for protein digestion to ensure full scan of acetyl-peptides.
MtoZ Biolabs offers protein ubiquitination identification and quantification service using high-resolution mass spectrometry analysis. We also use highly specific ubiquitin antibody for enrichment of low abundance ubiquitin-peptide.
MtoZ Biolabs utilizes the HCD/ETD mode of Orbitrap Fusion mass spectrometry for glycoprotein analysis. Coupled with Byonic software, we can accurately analyze N- and O-linked glycosylation sites and the corresponding glycogens.
MtoZ Biolabs provides disulfide bond analysis service at both single protein level and proteome level. We have optimized our sample preparation method to reduce the chance of in vitro disulfide bond exchange and maintain the native protein structure to the greatest extent.
MtoZ Biolabs has optimized our sample preparation protocols to obtain highly purified histones with the least effect to the modification, and ensures precise histone modification analysis using Nano LC-MS/MS.
*Note: We also provide custom analytical service to identify many other types of PTMs. For special requirement, please contact us for project discussion.
Sample Submission Requirements
*Customers are welcome to contact us for detailed sample requirements before sending your samples.
Case Study
In this study, the ubiquitination modification sites of a Co-IP protein sample are analyzed. Part of the final analytical results is listed as below, showing the peptide sequences and identified ubiquitination sites respectively.
Deliverables
1. Experiment Procedures
2. Parameters of Liquid Chromatography and Mass Spectrometer
3. MS Raw Data Files
4. Identification of Peptides with PTMs
Related Services
Protein Analysis
Protein Identification
Protein Mass Measurement
LC-MS Analysis of Pull-Down Proteins
Native MS Analysis
Protein Sequencing
Protein De Novo Seq
N-Terminal Sequencing
C-Terminal Sequencing
Edman Degradation
Protein Full-Length Sequencing
PTM Analysis
Phospho Proteomics
Acetyl Proteomics
Ubiquitin Proteomics
Glyco Proteomics
Disulfide Bond
Histone Modifications
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• Protein Trimethylation Analysis
Post-translational modifications (PTMs) refer to the chemical alterations that occur to proteins either during or after translation. PTMs are essential in regulating numerous cellular processes, including cell differentiation, protein degradation, signal transduction, gene expression regulation, and protein-protein interactions. Protein trimethylation, a key post-translational modification, involves the covalent attachment of three methyl groups (-CH3) to specific residues, typically lysine or arginine. Tri
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• Protein Butyrylation Analysis
Post-translational modifications (PTMs) refer to chemical changes made to proteins during or after their synthesis, playing critical roles in various cellular processes, including cell differentiation, protein degradation, signal transduction, gene expression regulation, and protein-protein interactions. Lysine butyrylation (Kbu), an emerging PTM, occurs through the covalent attachment of a butyryl group to lysine residues in proteins. This modification is crucial for regulating gene expression, metabolism,
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• Protein Methacrylation Analysis
At MtoZ Biolabs, we provide high-sensitivity Protein Methacrylation Analysis services using cutting-edge liquid chromatography-mass spectrometry (LC-MS/MS) technologies. Our advanced methods enable precise differentiation between these isomeric modifications, allowing researchers to uncover the unique roles of methacrylation across various biological processes.
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• Protein Glutarylation Analysis
MtoZ Biolabs, as an expert provider in proteomics and PTM analysis, offers high-sensitivity and high-specificity glutarylation analysis services utilizing advanced mass spectrometry (LC-MS/MS) platforms. Our meticulously designed workflows enable researchers to deeply explore the functional and regulatory roles of glutarylation in diverse biological contexts, thereby advancing studies in disease mechanisms and drug development.
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• Protein Crotonylation Analysis
MtoZ Biolabs offers specialized Protein Crotonylation Analysis services at both the targeted protein and proteomics levels. This allows researchers to either focus on specific targets or gain a broad understanding of crotonylation dynamics across the proteome, supporting detailed mechanistic studies and wide-ranging biological research.
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• Protein Lysine Methylation Analysis
MtoZ Biolabs utilizes advanced liquid chromatography-mass spectrometry (LC-MS/MS) platforms to offer highly sensitive and precise lysine methylation analysis. Our skilled team and state-of-the-art technology empower researchers to explore the biological significance and underlying mechanisms of lysine methylation in various cellular processes.
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• Protein Arginine Methylation Analysis
As an excellent provider of mass spectrometry analysis services, MtoZ Biolabs leverages advanced high-performance liquid chromatography-mass spectrometry (HPLC-MS/MS) platforms to provide precise and sensitive protein arginine methylation analysis. Our services not only identify methylation sites with high accuracy but also offer quantitative insights, enabling researchers to deeply explore the biological relevance of these modifications.
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• Protein Asymmetric Dimethylation Analysis
As a leading service provider in proteomics and PTMs, MtoZ Biolabs offers comprehensive Protein Asymmetric Dimethylation Analysis services. Our state-of-the-art platform integrates high-performance liquid chromatography (HPLC) with high-resolution mass spectrometry to deliver highly accurate and high-throughput data, supplemented by advanced bioinformatics solutions to help researchers decipher complex PTM networks.
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• Protein Symmetric Dimethylation Analysis
MtoZ Biolabs, utilizing its advanced mass spectrometry platform, provides comprehensive and accurate Protein Symmetric Dimethylation Analysis services. Through high-resolution mass spectrometry and targeted enrichment strategies, we support researchers in uncovering the roles of symmetric dimethylation in gene regulation, signaling pathways, and disease mechanisms.
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• Protein Dimethylation Analysis
MtoZ Biolabs, with its advanced mass spectrometry platform and extensive experience in PTM analysis, offers comprehensive Protein Dimethylation Analysis services. Utilizing high-resolution mass spectrometry and enrichment techniques, we accurately identify and quantify dimethylation sites, enabling researchers to explore the regulatory roles of dimethylation in cellular metabolism, gene expression, and disease pathways.
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