Post-Translational Modifications Proteomics Service
Post-translational modifications (PTMs) are covalent and usually enzymatic modifications on proteins. Common PTMs include phosphorylation, acetylation, ubiquitination, glycosylation, and so on. These PTMs can cause huge influences on protein structure, distribution, and function, hence, increasing the complexity of proteome to a greater extent. Protein PTMs is generally analyzed by measuring the mass increased on the modified peptides. Since PTMs are generally present in very low abundance, specific enrichment procedures are required before PTMs identification. MtoZ Biolabs has established a powerful and professional Post-Translational Modifications Proteomics Service, which includes Thermo Fisher Q ExactiveHF and Obitrap Fusion Lumos mass analyzer system, coupled with Nano-LC system. Our aim is to provide the most professional support for our clients’ research.
Analysis Workflow
PTMs Analysis Service Categories
MtoZ Biolabs provides protein phosphorylation analysis service with highly efficient phospho-peptides enrichment processing and Nano LC-MS/MS analysis. Coupled with SILAC/iTRAQ labeling, this service can be applied to large-scale phospho-proteomics analysis.
MtoZ Biolabs provides precise acetyl-proteomics analysis service using Nano LC-MS/MS. We also use CST acetylation-specific antibodies for acetyl-peptide enrichment, and 2-3 different enzymes for protein digestion to ensure full scan of acetyl-peptides.
MtoZ Biolabs offers protein ubiquitination identification and quantification service using high-resolution mass spectrometry analysis. We also use highly specific ubiquitin antibody for enrichment of low abundance ubiquitin-peptide.
MtoZ Biolabs utilizes the HCD/ETD mode of Orbitrap Fusion mass spectrometry for glycoprotein analysis. Coupled with Byonic software, we can accurately analyze N- and O-linked glycosylation sites and the corresponding glycogens.
MtoZ Biolabs provides disulfide bond analysis service at both single protein level and proteome level. We have optimized our sample preparation method to reduce the chance of in vitro disulfide bond exchange and maintain the native protein structure to the greatest extent.
MtoZ Biolabs has optimized our sample preparation protocols to obtain highly purified histones with the least effect to the modification, and ensures precise histone modification analysis using Nano LC-MS/MS.
*Note: We also provide custom analytical service to identify many other types of PTMs. For special requirement, please contact us for project discussion.
Sample Submission Requirements
*Customers are welcome to contact us for detailed sample requirements before sending your samples.
Case Study
In this study, the ubiquitination modification sites of a Co-IP protein sample are analyzed. Part of the final analytical results is listed as below, showing the peptide sequences and identified ubiquitination sites respectively.
Deliverables
1. Experiment Procedures
2. Parameters of Liquid Chromatography and Mass Spectrometer
3. MS Raw Data Files
4. Identification of Peptides with PTMs
Related Services
Protein Analysis
Protein Identification
Protein Mass Measurement
LC-MS Analysis of Pull-Down Proteins
Native MS Analysis
Protein Sequencing
Protein De Novo Seq
N-Terminal Sequencing
C-Terminal Sequencing
Edman Degradation
Protein Full-Length Sequencing
PTM Analysis
Phospho Proteomics
Acetyl Proteomics
Ubiquitin Proteomics
Glyco Proteomics
Disulfide Bond
Histone Modifications
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• Protein Deamidation Modification Analysis Service
Protein deamidation involves the conversion of amide groups (-CONH2) in amino acids like glutamic acid and aspartic acid into their corresponding acids, α-ketoglutarate and glutamic acid, by losing an ammonia molecule (NH3). This non-enzymatic post-translational modification is crucial for understanding proteins' charge distributions, functions, stability, and interactions with other molecules, as well as their roles in diseases.
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• Top-Down PTMs Analysis Service
Top-down mass spectrometry (TDMS) is highly effective for comprehensive analysis of protein post-translational modifications (PTMs) because it evaluates intact proteins or peptides without requiring hydrolysis. This method is particularly valuable in the analysis of biopharmaceutical proteins, such as monoclonal antibodies and recombinant proteins, where it preserves many unstable modifications that are often lost during collision-induced dissociation (CID) cleavage in shotgun approaches.
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Glycomics refers to the collection, analysis, and utilization of glycosidic biological data at the omics level. The profound biological effects of lipid and protein glycosylation make glycomics an important field in life sciences. N-glycome analysis (a comprehensive study of all glycans) is a complement to genomics and proteomics.
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• Quantitative Phosphoproteomics Service
MtoZ Biolabs provides an accurate all-inclusive phosphoproteomics service package, including protein labeling, phosphoprotein enrichment, MS analysis, and so on.
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• Quantitative Acetylproteomics Service
MtoZ Biolabs provides an accurate all-inclusive Acetyl-proteomics service package, including protein labeling, phosphoprotein enrichment, MS analysis, and so on.
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• Quantitative Ubiquitinomics Service
MtoZ Biolabs provides an accurate all-inclusive Ubiquitin-Proteomics service package, including protein labeling, phosphoprotein enrichment, MS analysis, and so on.
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• Quantitative Glycoproteomics Service
MtoZ Biolabs provides an accurate all-inclusive Glyco-Proteomics service package, including protein labeling, phosphoprotein enrichment, MS analysis, and so on.
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• Protein Disulfide Bonds Identification and Quantitative Analysis
MtoZ Biolabs has developed a specialized platform, equipped with Orbitrap Fusion Lumos mass spectrometers, and Nano-LC for disulfide bond analysis service.
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• Histone Post-Translational Modification Analysis Service
MtoZ Biolabs has developed a specialized platform, equipped with Orbitrap Fusion Lumos mass spectrometers, and Nano-LC for Histone modifications analysis service.
How to order?
