Resources

Proteomics Databases



Metabolomics Databases

• • Ion Exchange Chromatography Protein

  Ion Exchange Chromatography Protein Analysis is a commonly employed technique in protein separation and purification. This method capitalizes on differences in the surface charge of protein molecules to separate them using ion exchange chromatographic media. The fundamental principle involves utilizing the electrostatic attraction between charged stationary phases and protein molecules with opposite charges. Renowned for its efficiency and selectivity, this method finds widespread application in bioch......

• • Exosome Proteomics Sample Preparation

  1. Sample Collection: First, samples containing exosomes must be collected. This typically involves gathering from sources such as cell culture media, blood, urine, and cerebrospinal fluid, depending on the research objectives. For instance, tumor cell culture media is often collected for cancer studies. 2. Centrifugation and Ultracentrifugation: Once the samples are collected, exosomes are isolated through a series of centrifugation and ultracentrifugation steps. Initially, low-speed centrifugation........

• • How Many Proteins Can Be Detected by TMT in Proteomics

  Tandem Mass Tag (TMT) is a widely used high-throughput protein quantification technique in proteomics, designed to investigate protein expression differences across various samples. TMT enables the quantitative comparison of corresponding proteins between samples by labeling peptides with specific tags following protein hydrolysis.

• • What Is Used to Identify the Modification of a Specific Protein

  Specific protein modifications can be identified using a variety of methodologies, including: 1. Mass Spectrometry Fingerprinting This is a robust technique employed to identify proteins and their modifications. By examining the mass and distribution of proteins or peptide fragments, specific post-translational modifications such as phosphorylation, glycosylation, and ubiquitination can be accurately identified.

• • Which Type of Mass Spectrometry Is Best for Proteomics Identification

  Identification of protein modifications: LC-MS/MS can identify various chemical modifications of proteins, such as phosphorylation, acetylation, and ubiquitination, providing crucial information for studying protein function and regulatory mechanisms.

• • Principle of Mass Spectrometry for Detecting Ubiquitination

  The ubiquitin-proteasome pathway constitutes a major mechanism of protein degradation and plays a critical role in maintaining intracellular protein homeostasis. Ubiquitination, a post-translational modification, involves the covalent attachment of the small protein ubiquitin to target substrates. Mass spectrometry has emerged as a powerful and indispensable tool for investigating ubiquitination. In the following sections, we provide a detailed examination of the fundamental principles underlying the.......

• • How to Perform Protein Sequence Analysis?

  Protein sequence analysis involves examining the amino acid sequences of proteins to elucidate their structures and functions. This analytical approach transcends basic structural characterization, providing critical insights into protein functionality, molecular interactions, disease mechanisms, and therapeutic targets. Proteins exhibit remarkable diversity, with each protein's unique three-dimensional structure directly linked to its specific function, determined by its amino acid sequence. Understa......

• • Global Histone Post-Translational Modifications

  Histone post-translational modifications (PTMs) play a crucial role in the regulation of gene expression. By adding or removing chemical groups such as acetyl, phosphate, and methyl, these modifications can alter the charge distribution and conformation of histones. This in turn affects chromatin compaction and gene accessibility, facilitating precise control over gene expression.

• • Sugar Alcohol LC-MS Detection

  Sugar alcohols, also known as polyols, are a class of biologically significant natural products with diverse functions. They participate in numerous biochemical reactions within organisms and contribute to various physiological processes, including signal transduction, stabilization of cell membranes, antioxidative mechanisms, and stress responses. Consequently, sugar alcohols and their metabolites hold considerable importance in biochemical research, cell biology, and medical studies. Liquid chromatography

• • Total Redox-Modified Proteome

  Redox modification is an important form of protein modification within organisms, regulating the redox state of proteins and thereby controlling their structure, function, and activity. In general, redox modifications can be divided into oxidation modifications and reduction modifications.